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They were subsequently divided into three groups: sham, IR, and IR + RLN groups. Porcine RLN (500\u00a0ng\/h) or saline was infused using an implanted osmotic mini\u2010pump 90\u00a0min after inducing ischemia. The RLN dose used herein was that which resulted in serum RLN levels comparable to those in mid\u2010pregnant rats based on previous studies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Testicular IR increased germ cell apoptosis and histological damage as well as promoted disorganized and arrested spermatogenesis, accompanied by a significant increase in oxidative stress and inflammation. However, RLN administration ameliorated the adverse consequences associated with IR injury by attenuating oxidative stress and mitigating apoptosis and inflammation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion and conclusion<\/jats:title><jats:p>The study findings clearly demonstrated that RLN exerts a protective effect against IR\u2010induced testicular injury by attenuating oxidative stress, apoptosis, and inflammation, suggesting that RLN together with surgical treatment is a potentially efficacious approach toward ameliorating testicular dysfunction following testicular torsion.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.13096","type":"journal-article","created":{"date-parts":[[2021,8,26]],"date-time":"2021-08-26T06:42:04Z","timestamp":1629960124000},"page":"179-189","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Relaxin exerts a protective effect during ischemia\u2010reperfusion in the rat 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