{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:30:41Z","timestamp":1791419441723,"version":"4.3.3"},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T00:00:00Z","timestamp":1646697600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Glucose\u2010dependent insulinotropic polypeptide receptor (<jats:italic>Gipr<\/jats:italic>) gene expression has been reported in mouse spermatids and <jats:italic>Gipr<\/jats:italic> knockout male mice have previously been reported to have decreased in vitro fertilization, although the role of <jats:italic>Gipr<\/jats:italic> signaling in male mouse fertility is not well understood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The purposes of these studies were to determine the role of glucose\u2010dependent insulinotropic polypeptide receptor in male fertility using <jats:italic>Gipr<\/jats:italic> knockout mice and anti\u2010glucose\u2010dependent insulinotropic polypeptide receptor antibody\u2010treated wild\u2010type mice and to determine if the expression of <jats:italic>Gipr<\/jats:italic> in mouse testes is similar in non\u2010human and human primates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and materials<\/jats:title><jats:p>Adiponectin promoter\u2010driven <jats:italic>Gipr<\/jats:italic> knockout male mice (<jats:italic>Gipr<\/jats:italic><jats:sup>Adipo\u2013\/\u2013<\/jats:sup>) were assessed for in vitro and in vivo fertility, sperm parameters, and testicular histology. CD1 male mice were administered an anti\u2010glucose\u2010dependent insulinotropic polypeptide receptor antibody (muGIPR\u2010Ab) prior to and during mating for assessment of in vivo fertility and sperm parameters. Expression of <jats:italic>Gipr\/GIPR<\/jats:italic> mRNA in the mouse, cynomolgus monkey, and human testes was assessed by in situ hybridization methods using species\u2010specific probes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>Gipr<\/jats:italic><jats:sup>Adipo\u2013\/\u2013<\/jats:sup> male mice are infertile in vitro and in vivo, despite normal testis morphology, sperm counts, and sperm motility. In contrast, administration of muGIPR\u2010Ab to CD1 male mice did not impact fertility. While <jats:italic>Gipr<\/jats:italic> mRNA expression is detectable in the mouse testes, <jats:italic>GIPR<\/jats:italic> mRNA expression is not detectable in monkey or human testes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>The infertility of <jats:italic>Gipr<\/jats:italic><jats:sup>Adipo\u2013\/\u2013<\/jats:sup> male mice correlated with the lack of <jats:italic>Gipr<\/jats:italic> expression in the testis and\/or adipocyte tissue. However, as administration of muGIPR\u2010Ab did not impact the fertility of adult male mice, it is possible that the observations in genetically deficient male mice are related to <jats:italic>Gipr<\/jats:italic> deficiency during development.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our data support a role for <jats:italic>Gipr<\/jats:italic> expression in the mouse testis during the development of sperm fertilization potential, but based on gene expression data, a similar role for glucose\u2010dependent insulinotropic polypeptide receptor in non\u2010human primate or human male fertility is unlikely.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.13166","type":"journal-article","created":{"date-parts":[[2022,2,28]],"date-time":"2022-02-28T07:09:45Z","timestamp":1646032185000},"page":"789-799","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<i>GIPR<\/i> gene expression in testis is mouse specific and can impact male mouse fertility"],"prefix":"10.1111","volume":"10","author":[{"given":"Elizabeth A.","family":"Killion","sequence":"first","affiliation":[{"name":"Amgen Research Department of Cardiometabolic Disorders, Amgen Inc.  Thousand Oaks California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajaa","family":"Hussien","sequence":"additional","affiliation":[{"name":"Amgen Research, Department of Translational Safety and Bioanalytical Sciences Amgen Inc.  South San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Artem","family":"Shkumatov","sequence":"additional","affiliation":[{"name":"Amgen Research, Department of Translational Safety and Bioanalytical Sciences Amgen Inc.  South San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rhian","family":"Davies","sequence":"additional","affiliation":[{"name":"Amgen Research, Department of Translational Safety and Bioanalytical Sciences Amgen Inc.  South San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"Lloyd","sequence":"additional","affiliation":[{"name":"Amgen Research Department of Cardiometabolic Disorders, Amgen Inc.  Thousand Oaks California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Murielle M.","family":"V\u00e9niant","sequence":"additional","affiliation":[{"name":"Amgen Research Department of Cardiometabolic Disorders, Amgen Inc.  Thousand Oaks California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herve","family":"Lebrec","sequence":"additional","affiliation":[{"name":"Amgen Research, Department of Translational Safety and Bioanalytical Sciences Amgen Inc.  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