{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T13:26:46Z","timestamp":1791725206770,"version":"4.3.4"},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,7,11]],"date-time":"2022-07-11T00:00:00Z","timestamp":1657497600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Erectile function is usually impaired after radiation therapy in prostate cancer patients. eNOS is a key enzyme in the process of erection. Mitochondria\u2010associated membranes (MAMs) are closely contacted with the production and bioactivity of eNOS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To study the mechanism of icariin improves the erectile function of rats treated with prostate radiation by controling the expression of MAMs in penile corpus cavernosum.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twenty 8\u2010week\u2010old healthy male SD rats were randomized to four groups: control group, radiation therapy (RT) group, icariin (10\u00a0mg\/kg\/d gavage) group, and RT + icariin (10\u00a0mg\/kg\/d gavage) group (<jats:italic>n<\/jats:italic>\u00a0=\u00a05). In RT group and RT + icariin group, rats were irradiated with X\u2010rays to the prostate region (total dose 37.5 gray; 7.5 gray\/day for 5 days). The maximum intracavernous pressure\/mean arterial pressure (ICPmax\/MAP), NO concentration and the level of IP<jats:sub>3<\/jats:sub>R1, PACS2, FACL4, nNOS, p\u2010eNOS, and eNOS in rats\u2019 penile cavernous tissue was determined 9\u00a0weeks after radiation therapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared with the control group and the RT + icariin group, the ICPmax\/MAP of the RT group was remarkably reduced (<jats:italic>p\u00a0<\/jats:italic>&lt;\u00a00.05). The levels of p\u2010eNOS\/eNOS, nNOS and the concentration of NO in the penile cavernous tissue of the penis in the RT group were remarkably decreased compared to the control group and the RT + icariin group (<jats:italic>p\u00a0<\/jats:italic>&lt;\u00a00.05). The levels of IP<jats:sub>3<\/jats:sub>R1, PACS2, and FACL4 in penile cavernous tissue of the RT group were significantly higher than those in the control group and the RT + icariin group (<jats:italic>p\u00a0<\/jats:italic>&lt;\u00a00.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>After prostate X\u2010ray radiotherapy in rats, the formation of MAMs may be increased by increased expression of IP<jats:sub>3<\/jats:sub>R1, PACS2, and FACL4 in penile cavernous tissue, resulting in impaired erectile function. Icariin might increase p\u2010eNOS\/eNOS and improve erectile function in rats after prostate radiotherapy by inhibiting the expression of IP<jats:sub>3<\/jats:sub>R1, PACS2, and FACL4.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.13218","type":"journal-article","created":{"date-parts":[[2022,6,29]],"date-time":"2022-06-29T05:08:40Z","timestamp":1656479320000},"page":"1208-1216","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Icariin inhibits the formation of mitochondria\u2010associated membranes (MAMs) and improves erectile function in rats treated with prostate radiation"],"prefix":"10.1111","volume":"10","author":[{"given":"Chang\u2010Jian","family":"Deng","sequence":"first","affiliation":[{"name":"Department of Urology The Affiliated Hospital of Southwest Medical University  Luzhou 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