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Improvement was defined as an increase in the total motile progressive sperm count (TMC) of the post\u2010operative SA when compared to the baseline, and pregnancy was assessed for 24 months after MV.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Using linear discriminant analysis (LDA), we created a model that discriminated the men who improved SA from those who did not with accuracy of 93.1%. Another model segregated men who achieved natural pregnancy from men who did not. We identified seven metabolites that were important for group segregation: caprylate, isoleucine, N\u2010acetyltyrosine, carnitine, N\u2010acetylcarnitine, creatine, and threonine.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>We described the use of metabonomics model to predict with high accuracy the outcomes of MV in infertile men with varicocoele. The most important metabolites for group segregation are involved in energy metabolism and oxidative stress response, highlighting the pivotal role of these mechanisms in the pathophysiology of varicocoele.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:sup>1<\/jats:sup>H NMR spectroscopy of seminal plasma can be used in conjunction with multivariate statistical tools to create metabonomics models useful to segregate men with varicocoele based on the reproductive outcomes of MV. These models may help counseling infertile men with varicocoele regarding their prognosis after surgery.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.13281","type":"journal-article","created":{"date-parts":[[2022,8,26]],"date-time":"2022-08-26T17:47:46Z","timestamp":1661536066000},"page":"1581-1592","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Prediction of semen analysis parameter improvement after varicocoelectomy using 1 H NMR\u2010based metabonomics assays"],"prefix":"10.1111","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6783-307X","authenticated-orcid":false,"given":"Filipe Tenorio Lira","family":"Neto","sequence":"first","affiliation":[{"name":"Andros Recife, Recife, Brazil. Department of Urology, Instituto de Medicina Integral Prof. Fernando Figueira, Recife, Brazil. 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