{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T20:04:51Z","timestamp":1767902691506,"version":"3.49.0"},"reference-count":55,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,10,2]],"date-time":"2022-10-02T00:00:00Z","timestamp":1664668800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["JRP:\u00a0R01\u2010ES030942"],"award-info":[{"award-number":["JRP:\u00a0R01\u2010ES030942"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["R01\u2010ES028298"],"award-info":[{"award-number":["R01\u2010ES028298"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Currently, the precise mechanisms that underline male infertility are still unclear. Accumulating data implicate non\u2010coding RNA cargo of seminal plasma extracellular vesicles due to their association with poor semen quality and higher expression levels relative to vesicle\u2010free seminal plasma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>We assessed sperm\u2010free seminal plasma extracellular vesicle non\u2010coding RNA profiles from 91 semen samples collected from male participants of couples seeking infertility treatment. Men were classified into two groups (poor, <jats:italic>n<\/jats:italic>\u00a0=\u00a032; normal, <jats:italic>n<\/jats:italic>\u00a0=\u00a059) based on World Health Organization semen cutoffs. Small RNA sequencing reads were mapped to standard biotype\u2010specific transcriptomes in the order micro RNA\u00a0&gt;\u00a0transfer RNA\u00a0&gt;\u00a0piwi\u2010interacting RNA\u00a0&gt;\u00a0ribosomal RNA \u00a0&gt;\u00a0ribosomal RNA\u00a0&gt;\u00a0circular RNA\u00a0&gt;\u00a0long non\u2010coding RNA using STAR. Differential expression of normalized non\u2010coding RNA read counts between the two groups was conducted by EdgeR (Fold change \u22651.5 and (false discovery rate [FDR]\u00a0&lt;\u00a00.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Result<\/jats:title><jats:p>Small RNA sequencing identified a wide variety of seminal plasma extracellular vesicle non\u2010coding RNA biotypes including micro RNA, ribosomal RNAs, piwi\u2010interacting RNAs, transfer RNA, long non\u2010coding RNAs as well as circular RNAs, and fragments associated with pseudogenes, and nonsense\u2010mediated decay. The expression levels of 57 seminal plasma extracellular vesicle non\u2010coding RNAs (micro RNA: 6, piwi\u2010interacting RNA: 4, ribosomal RNA: 6, circular RNA: 34, and long non\u2010coding RNA: 7) were altered in men with poor semen quality relative to normal semen parameters, many (60%) of which were circular RNA species. Ontology analysis of differentially expressed micro RNAs and circular RNAs showed enrichment in functional terms related to cellular communication and early development.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This is the first study to generate comprehensive seminal plasma extracellular vesicle non\u2010coding RNA profiles in a clinical setting and to determine the differences between men with normal and abnormal semen parameters. Thus, our study suggests that seminal plasma extracellular vesicle non\u2010coding RNAs may represent novel biomarkers of male reproductive phenotypes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.13295","type":"journal-article","created":{"date-parts":[[2022,9,16]],"date-time":"2022-09-16T13:21:33Z","timestamp":1663334493000},"page":"677-686","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Altered non\u2010coding RNA profiles of seminal plasma extracellular vesicles of men with poor semen quality undergoing in vitro fertilization treatment"],"prefix":"10.1111","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6428-766X","authenticated-orcid":false,"given":"Oladele A.","family":"Oluwayiose","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology C.S. Mott Center for Human Growth and Development School of Medicine Wayne State University  Detroit Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emily","family":"Houle","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology C.S. Mott Center for Human Growth and Development School of Medicine Wayne State University  Detroit Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian W.","family":"Whitcomb","sequence":"additional","affiliation":[{"name":"Department of Biostatistics and Epidemiology School of Public Health and Health Sciences University of Massachusetts Amherst  Amherst Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Suvorov","sequence":"additional","affiliation":[{"name":"Department of Environmental Health Sciences School of Public Health and Health Sciences University of Massachusetts Amherst  Amherst Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tayyab","family":"Rahil","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Division of Reproductive Endocrinology and Infertility UMass Chan\u2013Baystate  Springfield Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cynthia K.","family":"Sites","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Division of Reproductive Endocrinology and Infertility UMass Chan\u2013Baystate  Springfield Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen A.","family":"Krawetz","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology C.S. Mott Center for Human Growth and Development School of Medicine Wayne State University  Detroit Michigan USA"},{"name":"Center for Molecular Medicine and Genetics Wayne State School of Medicine  Detroit Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pablo","family":"Visconti","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences University of Massachusetts Amherst  Amherst Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. Richard","family":"Pilsner","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology C.S. 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