{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T16:46:55Z","timestamp":1790095615850,"version":"4.0.1"},"reference-count":70,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,11,25]],"date-time":"2022-11-25T00:00:00Z","timestamp":1669334400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81871158"],"award-info":[{"award-number":["81871158"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The mechanism of high\u2010fat diet (HFD)\u2010induced decrease in erectile function has not been elucidated, and in previous studies, spectrin alpha, erythrocytic 1 (SPTA1) is a cytoskeletal protein that regulates cellular function, which belongs to a family of proteins that can affect cell and tissue growth and development by regulating YAP, an effector on the Hippo signaling pathway, but its particular role has not been elucidated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      To explore the role of\n                      <jats:italic>SPTA1<\/jats:italic>\n                      in the abnormality of erectile function induced by HFD.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We analyzed the penile tissues of mice on normal diet and HFD by transcriptomics and screened for differentially expressed genes, further identified closely related target genes in rat penile tissues, and verified target gene expression in in vitro construction of high\u2010glucose (HG)\u2010treated corpus cavernosum endothelial cells (CCECs) and corpus cavernosum smooth muscle cells (CCSMCs) models. The distribution of target genes in various cell populations in penile tissues was retrieved by single\u2010cell sequencing Male Health Atlas database. Moreover, interfering with target genes was further applied to explore the mechanisms involved in erectile function decline.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Transcriptomic analysis screened out down\u2010regulated differential gene\n                      <jats:italic>SPTA1<\/jats:italic>\n                      ; Western blot and immunohistochemistry results showed that SPTA1 expression significantly decreased in the penile tissues of Sprague\u2013Dawley (SD) rats in the HFD group. Immunofluorescence staining showed a positive expression of CD31 and VWF in CCECs and a positive expression of \u03b1\u2010SMA in CCSMCs. The expression level of SPTA1 protein significantly decreased in the HG group of CCECs and CCSMCs. The expression of\n                      <jats:italic>SPTA1<\/jats:italic>\n                      mRNA significantly decreased in CCSMCs while significantly increased in CCECs.\n                      <jats:italic>SPTA1<\/jats:italic>\n                      may have various expression patterns and biological functions in different cell populations. Real\u2010time quantitative PCR results showed that the siSPTA1 transfected in CCSMCs had a significant interference effect compared with the control siNC. Transfection of siSPTA1 into CCSMCs resulted in the significant down\u2010regulation of mRNA and protein expression of eNOS, and significant up\u2010regulation of YAP, Caspase\u20101, GSDMD, GSDMD\u2010N IL\u201018, and IL\u20101\u03b2 protein expression levels. The expression level of CCSMCs contractile\u2010type protein \u03b1\u2010SMA was significantly down\u2010regulated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The down\u2010regulation of SPTA1 in SD rats fed with HFD may induce cell pyroptosis and lead to the decrease of erectile function by activating the Hippo pathway; these findings may provide new therapeutic targets for improving erectile function.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/andr.13338","type":"journal-article","created":{"date-parts":[[2022,11,14]],"date-time":"2022-11-14T12:26:30Z","timestamp":1668428790000},"page":"591-610","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Cytoskeletal protein SPTA1 mediating the decrease in erectile function induced by high\u2010fat diet via Hippo signaling pathway"],"prefix":"10.1111","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4605-3717","authenticated-orcid":false,"given":"Ying","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Infertility and Sexual Medicine The Third Affiliated Hospital of Sun Yat\u2010sen University  Guangzhou China"},{"name":"Graduate School Guangxi University of Chinese Medicine  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Wang","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory for Crop Germplasm Resources Preservation and Utilization Agro\u2010biological Gene Research Center Guangdong Academy of Agricultural Sciences  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhan\u2010Sen","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Infertility and Sexual Medicine The Third Affiliated Hospital of Sun Yat\u2010sen 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