{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T16:26:12Z","timestamp":1791044772972,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,1,16]],"date-time":"2023-01-16T00:00:00Z","timestamp":1673827200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007449","name":"Takeda Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007449","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009633","name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["P01HD087157"],"award-info":[{"award-number":["P01HD087157"]}],"id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009633","name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["R01HD088412"],"award-info":[{"award-number":["R01HD088412"]}],"id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>TSN (translin), also called testis brain RNA\u2010binding protein, binds to TSNAX (translin\u2010associated factor X) and is suggested to play diverse roles, such as RNA metabolism and DNA damage response. TSNAXIP1 (Translin\u2010associated factor X\u2010interacting protein 1) was identified as a TSNAX\u2010interacting protein using a yeast two\u2010hybrid system, but its function in vivo was unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To reveal the function of TSNAXIP1 in vivo in mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>We generated <jats:italic>Tsnaxip1<\/jats:italic> knockout mice using the CRISPR\/Cas9 system and analyzed their fertility and sperm motility. Further, we generated <jats:italic>1700010I14Rik<\/jats:italic> knockout mice, because 1700010I14RIK is also predominantly expressed in testes and contains the same Pfam (protein families) domain as TSNAXIP1.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Reduced male fertility and impaired sperm motility with asymmetric flagellar waveforms were observed in not only <jats:italic>Tsnaxip1<\/jats:italic> but also <jats:italic>1700010I14Rik<\/jats:italic> knockout mice. Unlike <jats:italic>Tsn<\/jats:italic> knockout mice, no abnormalities were found in testicular sections of either <jats:italic>Tsnaxip1<\/jats:italic> or <jats:italic>1700010I14Rik<\/jats:italic> knockout mice. Furthermore, TSNAXIP1 was detected in the sperm tail and fractionated with axonemal proteins.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion and conclusion<\/jats:title><jats:p>Unlike the TSN\u2013TSNAX complex, whose disruption causes abnormal vacuoles in mouse testes, TSNAXIP1 and 1700010I14RIK may play roles in regulating sperm flagellar beating patterns.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.13378","type":"journal-article","created":{"date-parts":[[2023,1,4]],"date-time":"2023-01-04T12:59:43Z","timestamp":1672837183000},"page":"799-807","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Testis\u2010specific proteins, TSNAXIP1 and 1700010I14RIK, are important for sperm motility and male fertility in mice"],"prefix":"10.1111","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6300-4234","authenticated-orcid":false,"given":"Yuki","family":"Kaneda","sequence":"first","affiliation":[{"name":"Research Institute for Microbial Diseases Osaka University  Suita Osaka Japan"},{"name":"Graduate School of Pharmaceutical Sciences Osaka University  Suita Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4758-5803","authenticated-orcid":false,"given":"Haruhiko","family":"Miyata","sequence":"additional","affiliation":[{"name":"Research Institute for Microbial Diseases Osaka University  Suita Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3739-7163","authenticated-orcid":false,"given":"Keisuke","family":"Shimada","sequence":"additional","affiliation":[{"name":"Research Institute for Microbial Diseases Osaka University  Suita Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seiya","family":"Oura","sequence":"additional","affiliation":[{"name":"Research Institute for Microbial Diseases Osaka University  Suita Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9859-6217","authenticated-orcid":false,"given":"Masahito","family":"Ikawa","sequence":"additional","affiliation":[{"name":"Research Institute for Microbial Diseases Osaka University  Suita Osaka Japan"},{"name":"Graduate School of Pharmaceutical Sciences Osaka University  Suita Osaka Japan"},{"name":"The Institute of Medical Science The University of Tokyo  Minato\u2010ku Tokyo Japan"},{"name":"Center for Infectious Disease Education and Research Osaka University  Suita Osaka 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