{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T02:48:35Z","timestamp":1789181315053,"version":"build-2803163510"},"reference-count":58,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,2,24]],"date-time":"2023-02-24T00:00:00Z","timestamp":1677196800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000071","name":"National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["HD36239"],"award-info":[{"award-number":["HD36239"]}],"id":[{"id":"10.13039\/100000071","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000071","name":"National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["HD094546"],"award-info":[{"award-number":["HD094546"]}],"id":[{"id":"10.13039\/100000071","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Precise spatiotemporal expression of cohorts of differentiation markers unique to spermatogonia, spermatocytes, and round spermatids punctuates spermatogenesis and ensures its completion. For example, genes coding for the synaptonemal complex or the acrosome or flagellum are\u00a0expressed sequentially in a developmental stage\u2010 and germ cell\u2010specific manner. But the transcriptional mechanisms governing the spatiotemporal order of gene expression within the seminiferous epithelium are poorly understood. Using the round spermatid\u2010specific <jats:italic>Acrv1<\/jats:italic> gene, which codes for the acrosomal protein SP\u201010 as a model, we learned that (1) the proximal promoter itself contains all the necessary cis\u2010regulatory sequences, (2) an insulator prevents somatic cell expression of the testis\u2010specific gene, (3) RNA II polymerase is loaded on the <jats:italic>Acrv1<\/jats:italic> promoter but paused in spermatocytes, thus ensuring precise transcriptional elongation in round spermatids, and that (4) a transcriptional repressor binding protein of 43 kilodaltons (TDP\u201043) plays a role in\u00a0maintaining the paused state in spermatocytes. Although the Acrv1\u00a0enhancer element has been narrowed down to 50\u00a0bp and its binding to a 47\u00a0kDa testis\u2010abundant nuclear protein shown, the identity of the putative transcription factor responsible for activation of round spermatid\u2010specific transcription remains elusive. Human male infertility is idiopathic with limited treatment options. Understanding transcriptional regulation of spermatogenesis has the potential to lead to future therapies for male infertility.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.13410","type":"journal-article","created":{"date-parts":[[2023,2,16]],"date-time":"2023-02-16T07:04:02Z","timestamp":1676531042000},"page":"904-910","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Transcriptional regulation of spatiotemporal gene expression within the seminiferous epithelium: Mouse <i>Acrv1<\/i> gene as a model"],"prefix":"10.1111","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4891-8964","authenticated-orcid":false,"given":"Prabhakara P.","family":"Reddi","sequence":"first","affiliation":[{"name":"Department of Comparative Biosciences University of Illinois Urbana Champaign  Urbana Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,2,24]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1000990303"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/scdb.1998.0201"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.103.026880"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2018.07.025"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.01.045"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.10.026"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1952.tb26576.x"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12722"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-038-0_27"},{"key":"e_1_2_4_11_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod61.5.1256"},{"key":"e_1_2_4_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0378(01)00104-8"},{"key":"e_1_2_4_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.1020150603"},{"key":"e_1_2_4_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/47412"},{"key":"e_1_2_4_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/3.6.1463"},{"key":"e_1_2_4_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(99)01516-9"},{"key":"e_1_2_4_17_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.176826.111"},{"key":"e_1_2_4_18_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-genet-030220-015007"},{"key":"e_1_2_4_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.23.15.5354-5365.2003"},{"key":"e_1_2_4_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2012.233"},{"key":"e_1_2_4_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.08.069"},{"key":"e_1_2_4_22_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.14-267328"},{"key":"e_1_2_4_23_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.110.088955"},{"key":"e_1_2_4_24_1","doi-asserted-by":"publisher","DOI":"10.1210\/endocr\/bqaa210"},{"key":"e_1_2_4_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0070-2153(05)71005-X"},{"key":"e_1_2_4_26_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1336.025"},{"key":"e_1_2_4_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/380162a0"},{"key":"e_1_2_4_28_1","doi-asserted-by":"publisher","DOI":"10.2741\/A131"},{"key":"e_1_2_4_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/386713a0"},{"key":"e_1_2_4_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0408718102"},{"key":"e_1_2_4_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scr.2017.07.026"},{"key":"e_1_2_4_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep28355"},{"key":"e_1_2_4_33_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod42.2.377"},{"key":"e_1_2_4_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.22358"},{"key":"e_1_2_4_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2007.06.001"},{"key":"e_1_2_4_36_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod53.4.873"},{"key":"e_1_2_4_37_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod51.6.1222"},{"key":"e_1_2_4_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(99)02026-1"},{"key":"e_1_2_4_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioz115"},{"key":"e_1_2_4_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2006.04.443"},{"key":"e_1_2_4_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.69.6.3584-3596.1995"},{"key":"e_1_2_4_42_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.23.14990"},{"key":"e_1_2_4_43_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M104236200"},{"key":"e_1_2_4_44_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA118.007222"},{"key":"e_1_2_4_45_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.166587"},{"key":"e_1_2_4_46_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-15-0239"},{"key":"e_1_2_4_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.embor.embor738"},{"key":"e_1_2_4_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(88)91087-2"},{"key":"e_1_2_4_49_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.325142.119"},{"key":"e_1_2_4_50_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.14.7.792"},{"key":"e_1_2_4_51_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M104967200"},{"key":"e_1_2_4_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2021.101231"},{"key":"e_1_2_4_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.22389"},{"key":"e_1_2_4_54_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.291.5503.447"},{"key":"e_1_2_4_55_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.954702"},{"key":"e_1_2_4_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0012-1606(03)00349-X"},{"key":"e_1_2_4_57_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M705811200"},{"key":"e_1_2_4_58_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddq137"},{"key":"e_1_2_4_59_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD14090"}],"container-title":["Andrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/andr.13410","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/andr.13410","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/andr.13410","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T06:01:20Z","timestamp":1692424880000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/andr.13410"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,24]]},"references-count":58,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023,7]]}},"alternative-id":["10.1111\/andr.13410"],"URL":"https:\/\/doi.org\/10.1111\/andr.13410","archive":["Portico"],"relation":{},"ISSN":["2047-2919","2047-2927"],"issn-type":[{"value":"2047-2919","type":"print"},{"value":"2047-2927","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,24]]},"assertion":[{"value":"2022-10-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}