{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:44:06Z","timestamp":1787013846677,"version":"build-2736575974"},"reference-count":48,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2023,3,21]],"date-time":"2023-03-21T00:00:00Z","timestamp":1679356800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["R01ES030942"],"award-info":[{"award-number":["R01ES030942"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011495","name":"Massachusetts Life Sciences Center","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011495","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Phthalates have been linked to adverse male reproductive health, including poor sperm quality and embryo quality as well as a longer time to pregnancy (months of unprotected intercourse before conception occurs). The present study aimed to evaluate the effect of preconception exposure to two ubiquitous phthalate chemicals, di(2\u2010ethylhexyl) phthalate (DEHP), di\u2010n\u2010butyl phthalate (DBP), and their mixture on sperm function, fertilization, and embryo development in mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and methods<\/jats:title>\n                    <jats:p>Adult male C57BL\/6J\u00a0mice aged 8\u20139\u00a0weeks were exposed to di(2\u2010ethylhexyl) phthalate, di\u2010n\u2010butyl phthalate, or their mixture (di\u2010n\u2010butyl phthalate\u00a0+\u00a0di(2\u2010ethylhexyl) phthalate) at 2.5\u00a0mg\/kg\/day or vehicle for 40\u00a0days (equivalent to one spermatogenic cycle) via surgically implanted osmotic pumps. Caudal epididymal spermatozoa were extracted and analyzed for motility using computer\u2010assisted sperm analyses. Sperm phosphorylation of protein kinase A substrates and tyrosine phosphorylation, markers of early and late capacitation events, respectively, were analyzed by Western blots. In vitro fertilization was used to evaluate the sperm fertilizing capacity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>While the study did not reveal any significant differences in sperm motility and fertilization potential, abnormal sperm morphology was observed in all phthalate exposures, particularly in the phthalate mixture group. In addition, the study revealed significant differences in sperm concentration between control and exposed groups. Moreover, protein phosphorylation of protein kinase A substrates was decreased in the di(2\u2010ethylhexyl) phthalate and mixture exposure groups, while no significant changes in protein tyrosine phosphorylation were observed in any of the groups. Assessment of the reproductive functionality did not reveal significant effects on in vitro fertilization and early embryo development rates but showed wide variability in the phthalate mixture group.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our findings suggest that preconception phthalate exposure affects sperm numbers and phosphorylation of protein kinase A substrates involved in capacitation. Future research is warranted to examine the associations between phthalate exposure and capacitation in human spermatozoa.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/andr.13423","type":"journal-article","created":{"date-parts":[[2023,3,9]],"date-time":"2023-03-09T03:23:50Z","timestamp":1678332230000},"page":"1484-1494","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Effects of preconception exposure to phthalates on mouse sperm capacitation parameters"],"prefix":"10.1111","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0857-5509","authenticated-orcid":false,"given":"Gayatri","family":"Mohanty","sequence":"first","affiliation":[{"name":"Department of Veterinary and Animal Sciences University of Massachusetts  Amherst Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darya A.","family":"Tourzani","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences University of Massachusetts  Amherst Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda G.","family":"Gervasi","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences University of Massachusetts  Amherst Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Houle","sequence":"additional","affiliation":[{"name":"C.S. Mott Center for Human Growth and Development Department of Obstetrics and Gynecology School of Medicine Wayne State University  Detroit Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6428-766X","authenticated-orcid":false,"given":"Oladele","family":"Oluwayiose","sequence":"additional","affiliation":[{"name":"C.S. Mott Center for Human Growth and Development Department of Obstetrics and Gynecology School of Medicine Wayne State University  Detroit Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Suvorov","sequence":"additional","affiliation":[{"name":"Department of Environmental Health Sciences School of Public Health and Health Sciences University of Massachusetts Massachusetts  Amherst USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4541-6267","authenticated-orcid":false,"given":"J. Richard","family":"Pilsner","sequence":"additional","affiliation":[{"name":"C.S. Mott Center for Human Growth and Development Department of Obstetrics and Gynecology School of Medicine Wayne State University  Detroit Michigan USA"},{"name":"Institute of Environmental Health Sciences Wayne State University  Detroit Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9320-7518","authenticated-orcid":false,"given":"Pablo E.","family":"Visconti","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences University of Massachusetts  Amherst Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,3,21]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.4103\/0974-1208.170370"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmac035"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-39913-9"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/1557988316643383"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmx022"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1472-6483(10)60530-5"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep31281"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dex283"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph17020489"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2018.07.029"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1100\/2012\/615068"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envres.2018.01.023"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12124"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/del428"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-48441-5"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-80857-2"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfq147"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2021.106693"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2018.00060"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.114.127266"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioy132"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.085845"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aaz4796"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-006-9022-8"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2018.06.024"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2018.06.023"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2017.01.034"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2019.04.014"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.06.029"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3889"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2003.09.063"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2006.05.057"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deq103"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deh127"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2021.152746"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2010.11.028"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2011.09.011"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11934-012-0261-1"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.4103\/0976-0105.177703"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.2903\/j.efsa.2019.5838"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.119834"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.105.049254"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.22663"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2005.06.007"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0405580101"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.02.056"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201438869"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envres.2022.114115"}],"container-title":["Andrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/andr.13423","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/andr.13423","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/andr.13423","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,8]],"date-time":"2023-10-08T14:45:33Z","timestamp":1696776333000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/andr.13423"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,21]]},"references-count":48,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2023,10]]}},"alternative-id":["10.1111\/andr.13423"],"URL":"https:\/\/doi.org\/10.1111\/andr.13423","archive":["Portico"],"relation":{},"ISSN":["2047-2919","2047-2927"],"issn-type":[{"value":"2047-2919","type":"print"},{"value":"2047-2927","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,21]]},"assertion":[{"value":"2022-09-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}