{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:29:28Z","timestamp":1791419368525,"version":"4.3.3"},"reference-count":37,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,4,11]],"date-time":"2023-04-11T00:00:00Z","timestamp":1681171200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Sperm chromatin dispersion test is a common and inexpensive technique to assess sperm DNA fragmentation, but its subjectivity in assessing a small number of spermatozoa is a disadvantage.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To study the efficacy of a new sperm chromatin dispersion test kit (R10) combined with an artificial intelligence\u2010aided halo\u2010evaluation platform (X12) and compare the results to those of existing sperm DNA fragmentation testing methods.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Semen samples from normozoospermic donors (<jats:italic>n<\/jats:italic>\u00a0=\u00a010) and infertile men with abnormal semen parameters (<jats:italic>n<\/jats:italic>\u00a0=\u00a010) were enrolled. DNA fragmentation indices were examined by multiple assays, including R10, Halosperm G2 (G2), sperm chromatin structure assay, and terminal deoxynucleotidyl transferase deoxynucleotidyl transferase nick end labeling. In R10 assay, the DNA fragmentation indices were obtained both manually (manual R10) and by X12 (AI\u2010R10). The obtained DNA fragmentation indices were analyzed by agreement analyses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The DNA fragmentation indices obtained by manual R10 and those obtained by AI\u2010R10 showed a strong significant correlation (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.97, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001) and agreement. The number of spermatozoa evaluated by AI\u2010R10 was 2078 (680\u20135831). The DNA fragmentation indices obtained by manual R10 and AI\u2010R10 both correlated with those of G2 (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.90, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001; <jats:italic>r<\/jats:italic>\u00a0=\u00a00.88, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001). Between the AI\u2010R10 and G2 results, Passing\u2013Bablok regression showed no systematic or proportional difference, and Bland\u2013Altman plots revealed overall agreement and a mean bias of 6.3% with an SD of 6.9% (95% limit of agreement: \u22127.2% to 19.9%). AI\u2010R10 and sperm chromatin structure assays showed systematic differences with a mean bias of \u22121.9%, while AI\u2010R10 and terminal deoxynucleotidyl transferase deoxynucleotidyl transferase nick end labeling revealed proportional differences with a mean bias of \u221210.7%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The novel sperm chromatin dispersion kit and artificial intelligence\u2010aided platform demonstrated significant correlation and agreement with existing sperm chromatin dispersion methods by assessing greater number of spermatozoa. This technique has the potential to provide a rapid and accurate assessment of sperm DNA fragmentation without technical expertise or flow cytometry.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.13436","type":"journal-article","created":{"date-parts":[[2023,4,1]],"date-time":"2023-04-01T06:54:56Z","timestamp":1680332096000},"page":"1581-1592","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Novel sperm chromatin dispersion test with artificial intelligence\u2010aided halo evaluation: A comparison study with existing modalities"],"prefix":"10.1111","volume":"11","author":[{"given":"Shinnosuke","family":"Kuroda","sequence":"first","affiliation":[{"name":"Department of Urology Glickman Urological &amp; Kidney Institute, Cleveland Clinic Foundation  Cleveland Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keshab Kumar","family":"Karna","sequence":"additional","affiliation":[{"name":"Department of Molecular Cell and Cancer Biology, University of Massachusetts Chan Medical School  Worcester Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raneen Sawaid","family":"Kaiyal","sequence":"additional","affiliation":[{"name":"Department of Urology Glickman Urological &amp; Kidney Institute, Cleveland Clinic Foundation  Cleveland Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rossella","family":"Cannarella","sequence":"additional","affiliation":[{"name":"Department of Urology Glickman Urological &amp; Kidney Institute, Cleveland Clinic Foundation  Cleveland Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott D.","family":"Lundy","sequence":"additional","affiliation":[{"name":"Department of Urology Glickman Urological &amp; Kidney Institute, Cleveland Clinic Foundation  Cleveland Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah C.","family":"Vij","sequence":"additional","affiliation":[{"name":"Department of Urology Glickman Urological &amp; Kidney Institute, Cleveland Clinic Foundation  Cleveland Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashok","family":"Agarwal","sequence":"additional","affiliation":[{"name":"Global Andrology Forum American Center for Reproductive Medicine  Moreland Hills Ohio USA"},{"name":"Cleveland Clinic Foundation  Cleveland Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,4,11]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2018.03.012"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(20)32667\u20102"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2014.10.021"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2022.05.036"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11845-017-1708-7"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2047\u20102927.2012.00041.x"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2011.05.026"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815\u2010006\u20109066\u20109"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2014.06.033"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/des261"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.12910"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/aja.2013.65"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.14275"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/hropen\/hoy004"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2021.08.014"},{"key":"e_1_2_10_17_1","volume-title":"WHO Laboratory Manual for the Examination and Processing of Human Semen","author":"World Health Organization","year":"2021"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.21037\/tau\u201022\u2010149"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u201060327\u2010409\u20108_21"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2004.11.089"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.13738"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/aja.2010.73"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.4103\/CJP.CJP_12_22"},{"key":"e_1_2_10_24_1","volume-title":"WHO Laboratory Manual for the Examination and Processing of Human Semen","author":"World Health Organization","year":"2010"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2016.05.013"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2018.06.023"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815\u2010015\u20100635\u20107"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939\u20104640.2002.tb02599.x"},{"key":"e_1_2_10_29_1","first-page":"147","article-title":"Sperm chromatin structure assay (SCSA\u00ae)","volume":"927","author":"Evenson DP","year":"2013","journal-title":"Methods Cell Biol"},{"issue":"11","key":"e_1_2_10_30_1","first-page":"709","article-title":"A new biometrical procedure for testing the equality of measurements from two different analytical methods. Application of linear regression procedures for method comparison studies in clinical chemistry, part I","volume":"21","author":"Passing H","year":"1983","journal-title":"J Clin Chem Clin Biochem"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00483\u201018"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aju.2017.11.002"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2018.12.029"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0165125"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.13874"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.21037\/tau.2017.08.06"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/S11934\u2010019\u20100914\u20104"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11081563"}],"container-title":["Andrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/andr.13436","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T05:49:34Z","timestamp":1698385774000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/andr.13436"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,11]]},"references-count":37,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2023,11]]}},"alternative-id":["10.1111\/andr.13436"],"URL":"https:\/\/doi.org\/10.1111\/andr.13436","archive":["Portico"],"relation":{},"ISSN":["2047-2919","2047-2927"],"issn-type":[{"value":"2047-2919","type":"print"},{"value":"2047-2927","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,11]]},"assertion":[{"value":"2023-01-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}