{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T10:26:38Z","timestamp":1789986398728,"version":"4.0.1"},"reference-count":40,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2023,4,27]],"date-time":"2023-04-27T00:00:00Z","timestamp":1682553600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011789","name":"Department of Science and Technology of Jilin Province","doi-asserted-by":"publisher","award":["20200201419"],"award-info":[{"award-number":["20200201419"]}],"id":[{"id":"10.13039\/501100011789","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011789","name":"Department of Science and Technology of Jilin Province","doi-asserted-by":"publisher","award":["YDZJ202301ZYTS138"],"award-info":[{"award-number":["YDZJ202301ZYTS138"]}],"id":[{"id":"10.13039\/501100011789","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2023,10]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n Erectile dysfunction (ED) and weakness of the penis are processes related to hemodynamic alteration. Low\u2010intensity pulsed ultrasound (LIPUS), as a new mechanical modality for the treatment of ED, deserves to be explored in depth for the biomechanical mechanisms it exerts.<\/jats:p>\n <\/jats:sec>\n \n Objective<\/jats:title>\n The aim of this study was to explore the role of YAP\/TAZ\u2010mediated mechanotransduction in mechanical therapy for the treatment of neurogenic erectile dysfunction (NED).<\/jats:p>\n <\/jats:sec>\n \n Materials and methods<\/jats:title>\n \n Forty\u2010two male SD rats (12 w old) were randomly divided into sham\u2010operated (\n n<\/jats:italic>\n \u00a0=\u00a014), bilateral cavernous nerve injury (BCNI,\n n<\/jats:italic>\n \u00a0=\u00a014), and LIPUS\u2010treated (\n n<\/jats:italic>\n \u00a0=\u00a014) groups. Intracavernosal pressure\/mean arterial pressure (ICP\/MAP) was measured 14 and 28 days after treatment. Penile tissue specimens were collected for pathological examination, and the changes in YAP, TAZ, connective tissue growth factor (CTGF), CYR61, LATS1, and p38 mitogen\u2010activated protein kinase expression levels were assessed by Western blot, real\u2010time quantitative polymerase chain reaction (RT\u2013qPCR) and immunological staining.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Compared with BCNI, LIPUS significantly improved ICP\/MAP levels and enhanced histopathological changes. The penile expression levels of YAP, TAZ, CTGF, and CYR61 were significantly downregulated in the BCNI group (\n p<\/jats:italic>\n \u00a0<\u00a00.01), and LIPUS upregulated the expression levels of these proteins (\n p<\/jats:italic>\n \u00a0<\u00a00.05). The expression levels of p\u2010LATS1 and LATS1 were not significantly different among the groups (\n p<\/jats:italic>\n \u00a0>\u00a00.05). Interestingly, the expression level of p\u2010p38\/p38 significantly increased in BCNI rats (\n p<\/jats:italic>\n \u00a0<\u00a00.05), which was reversed by LIPUS treatment (\n p<\/jats:italic>\n \u00a0<\u00a00.05). However, the p38 inhibitor SB203580 did not change the expression of YAP\/TAZ in rat primary smooth muscle cells or mouse MOVAS cells (\n p<\/jats:italic>\n \u00a0>\u00a00.05).\n <\/jats:p>\n <\/jats:sec>\n \n Discussion and conclusion<\/jats:title>\n LIPUS can effectively improve penile erectile function in NED rats. The underlying mechanism may be related to the regulation of YAP\/TAZ\u2010mediated mechanotransduction. However, the upstream regulatory signal may differ from the classical Hippo pathway.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/andr.13438","type":"journal-article","created":{"date-parts":[[2023,4,12]],"date-time":"2023-04-12T03:55:15Z","timestamp":1681271715000},"page":"1514-1527","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Role of mechanotransduction mediated by YAP\/TAZ in the treatment of neurogenic erectile dysfunction with low\u2010intensity pulsed ultrasound"],"prefix":"10.1111","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4702-5809","authenticated-orcid":false,"given":"Yang","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Urology The Affiliated Hospital of Changchun University of Chinese Medicine Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010ying","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Urology The Affiliated Hospital of Changchun University of Chinese Medicine Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang\u2010xiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Urology The Affiliated Hospital of Changchun University of Chinese Medicine Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji\u2010lei","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Urology The Affiliated Hospital of Changchun University of Chinese Medicine Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yin\u2010hui","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Urology The Affiliated Hospital of Changchun University of Chinese Medicine Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2907-3030","authenticated-orcid":false,"given":"Yong","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Urology The Affiliated Hospital of Changchun University of Chinese Medicine Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1285-9328","authenticated-orcid":false,"given":"Zhi\u2010tao","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Urology The Affiliated Hospital of Changchun University of Chinese Medicine Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,4,27]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0140\u20106736(12)60520\u20100"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ijir.3901199"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sxmr.2018.10.005"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.REV119.007963"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580\u2010018\u20100086\u2010y"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41556\u2010022\u201000848\u20105"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00005.2014"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2017.87"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2021.05.033"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/1878\u20100261.13061"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells8050407"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsif.2007.0221"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2015.02.029"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/6202842"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.21037\/tau.2016.02.04"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.4103\/aja.aja_95_19"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2015.07.026"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.3760\/cma.j.cn112137\u201020191207\u201002679"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/CM9.0000000000001431"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2020.102656"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.21037\/tau\u201022\u2010376"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2020.08.008"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2022.113109"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/jsm.12457"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2019.00049"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2015.08.005"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17071057"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mehy.2018.10.014"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0104863"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.274027.115"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0101963"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.01.029"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2014.0380"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.12309"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11255\u2010018\u20101989\u20104"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.esxm.2019.08.005"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.CAN\u201016\u20100155"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.112364"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.07.020"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2019.7763"}],"container-title":["Andrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/andr.13438","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,8]],"date-time":"2023-10-08T14:46:49Z","timestamp":1696776409000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/andr.13438"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,27]]},"references-count":40,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2023,10]]}},"alternative-id":["10.1111\/andr.13438"],"URL":"https:\/\/doi.org\/10.1111\/andr.13438","archive":["Portico"],"relation":{},"ISSN":["2047-2919","2047-2927"],"issn-type":[{"value":"2047-2919","type":"print"},{"value":"2047-2927","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,27]]},"assertion":[{"value":"2022-11-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}