{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T02:31:54Z","timestamp":1772591514330,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,5,27]],"date-time":"2023-05-27T00:00:00Z","timestamp":1685145600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HD043945"],"award-info":[{"award-number":["HD043945"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AG029531"],"award-info":[{"award-number":["AG029531"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HD103384"],"award-info":[{"award-number":["HD103384"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>In the mouse testis, Sertoli cells rapidly divide during a narrow window of time pre\u2010pubertally and differentiate thereafter. The number of Sertoli cells determines the testis size and germ cell\u2010carrying capacity. Follicle\u2010stimulating hormone (FSH) binds its cognate FSH\u2010receptors expressed on Sertoli cells and acts as a mitogen to regulate their proliferation. <jats:italic>Fshb<jats:sup>\u2212\/\u2212<\/jats:sup><\/jats:italic> mutant adult male mice have reduced Sertoli cell number and testis size and reduced sperm number and motility. However, FSH\u2010responsive genes in early postnatal mouse Sertoli cells are unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To identify FSH\u2010responsive genes in early postnatal mouse Sertoli cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>A fluorescence\u2010activated cell sorting method was developed to rapidly purify Sertoli cells from control and <jats:italic>Fshb<jats:sup>\u2212\/\u2212<\/jats:sup><\/jats:italic> mice carrying a <jats:italic>Sox9 <jats:sup>GfpKI<\/jats:sup><\/jats:italic> allele. These pure Sertoli cells were used for large\u2010scale gene expression analyses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We show that mouse Sertoli cells rarely divide beyond postnatal day 7. Our in vivo BrdU labeling studies indicate loss of FSH results in a 30% reduction in Sertoli cell proliferation in mice at 5 days of age. Flowsorted GFP<jats:sup>+<\/jats:sup> Sertoli cells with maximal <jats:italic>Fshr<\/jats:italic> expression were 97%\u201098% pure and mostly devoid of Leydig and germ cells as assessed by Taqman qPCR quantification of gene expression and immunolabeling of the corresponding cell\u2010specific markers. Large\u2010scale gene expression analysis identified several differentially regulated genes in flow\u2010sorted GFP<jats:sup>+<\/jats:sup> Sertoli cells obtained from testis of control and <jats:italic>Fshb<jats:sup>\u2212\/\u2212<\/jats:sup><\/jats:italic> mice at 5 days of age. The top 25 networks identified by pathway analysis include those related to the cell cycle, cell survival and most importantly, carbohydrate and lipid metabolism and molecular transport.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>Several of the FSH\u2010responsive genes identified in this study could serve as useful markers for Sertoli cell proliferation in normal physiology, toxicant\u2010induced Sertoli cell\/testis injury, and other pathological conditions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our studies reveal that FSH\u2010regulates macromolecular metabolism and molecular transport networks of genes in early postnatal Sertoli cells most likely in preparation for establishment of functional associations with germ cells to successfully coordinate spermatogenesis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.13459","type":"journal-article","created":{"date-parts":[[2023,5,20]],"date-time":"2023-05-20T04:43:57Z","timestamp":1684557837000},"page":"860-871","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Identification of follicle\u2010stimulating hormone\u2010responsive genes in Sertoli cells during early postnatal mouse testis development"],"prefix":"10.1111","volume":"11","author":[{"given":"Huizhen","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Cell Biology and Physiology University of Kansas Medical Center  Kansas City Kansas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenghui","family":"Liu","sequence":"additional","affiliation":[{"name":"Division of Reproductive Sciences Department of Obstetrics and Gynecology University of Colorado Anschutz Medical Campus  Aurora Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Larsen","sequence":"additional","affiliation":[{"name":"Division of Reproductive Sciences Department of Obstetrics and Gynecology University of Colorado Anschutz Medical Campus  Aurora Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Hastings","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Physiology University of Kansas Medical Center  Kansas City Kansas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sumedha","family":"Gunewardena","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Physiology University of Kansas Medical Center  Kansas City Kansas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7666-5493","authenticated-orcid":false,"given":"T. Rajendra","family":"Kumar","sequence":"additional","affiliation":[{"name":"Division of Reproductive Sciences Department of Obstetrics and Gynecology University of Colorado Anschutz Medical Campus  Aurora Colorado USA"},{"name":"Division of Reproductive Endocrinology and Infertility Department of Obstetrics and Gynecology University of Colorado Anschutz Medical Campus  Aurora Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,5,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1998.9068"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.149203"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12165"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/scdb.1998.0203"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioy027"},{"key":"e_1_2_9_7_1","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/B978-0-323-47912-7.00002-0","volume-title":"Yen & Jaffe's Reproductive Endocrinology","author":"Narayan P","year":"2019"},{"key":"e_1_2_9_8_1","first-page":"1","volume-title":"Endocrinology of the Testis and Male Reproduction","author":"Ulloa\u2010Aguirre A","year":"2017"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.105.050450"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.111.095091"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/15384101.2016.1207835"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diff.2021.06.002"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0297-201"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.142.8.8336"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.142.7.8230"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2002\u20100059"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2014\u20101356"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0216586"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2003\u20100203"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2003\u20100188"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.110.086900"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.21954"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2016.08.031"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2022.111641"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.621565"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2015.10.006"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2003-1055"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.23406"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010genet\u2010080320\u2010040045"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2020.05.010"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0191201"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1321404111"}],"container-title":["Andrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/andr.13459","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T06:00:38Z","timestamp":1692424838000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/andr.13459"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,27]]},"references-count":31,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023,7]]}},"alternative-id":["10.1111\/andr.13459"],"URL":"https:\/\/doi.org\/10.1111\/andr.13459","archive":["Portico"],"relation":{},"ISSN":["2047-2919","2047-2927"],"issn-type":[{"value":"2047-2919","type":"print"},{"value":"2047-2927","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,27]]},"assertion":[{"value":"2022-12-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}