{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T22:41:06Z","timestamp":1778539266929,"version":"3.51.4"},"reference-count":67,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,7,5]],"date-time":"2023-07-05T00:00:00Z","timestamp":1688515200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The concerning trend on male infertility global prevalence, together with the unexplainable causes in half of those cases, highlights that there are still aspects of this disease to be understood and solved. To address this issue, one should not only be aware of the limitations of the implemented diagnostic tools, but also understand the sperm cell in depth, structurally, biochemically, molecularly in order to develop reliable and ready\u2010to\u2010be new\/improved diagnostic tools. In this sense, the sperm cells metabolism, highly related to its functionality, seems to be a promising aspect to explore. Though there is much information on the human sperm metabolism, there is still a lack of a quick integrated and comprehensive analysis that may be introduced with the potential to reveal innovative clinically relevant information.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Find metabolic details on human sperm that can be accessed easily, in real time and using few cells, relying on the bivalent potential of the Seahorse flux analyzer (SFA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We have obtained standard records on human sperm cells\u2019 oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), that together with the metabolic metrics provided information on sperm cells\u2019 oxidative and glycolytic metabolism. Furthermore, a metabolic interindividual variation was observed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion and conclusion<\/jats:title><jats:p>Although the comparison with other species or cell types is not linear and warrant further studies, the metabolic profile of human sperm cells seems to be similar to that of other species. Altogether our results corroborate the value of SFA for metabolic human sperm cell analysis, warranting new studies, and anticipating several applications in the male infertility field.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.13486","type":"journal-article","created":{"date-parts":[[2023,6,26]],"date-time":"2023-06-26T06:24:39Z","timestamp":1687760679000},"page":"410-421","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Metabolic characterization of human sperm cells using the Seahorse metabolic flux analyzer"],"prefix":"10.1111","volume":"12","author":[{"given":"Artur","family":"Freitas\u2010Martins","sequence":"first","affiliation":[{"name":"Department of Life Sciences University of Coimbra Coimbra Portugal"},{"name":"CNC\u2014Centre for Neuroscience and Cell Biology University of Coimbra Coimbra Portugal"},{"name":"IIIUC\u2014Institute for Interdisciplinary Research University of Coimbra Coimbra Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria In\u00eas","family":"Sousa","sequence":"additional","affiliation":[{"name":"Department of Life Sciences University of Coimbra Coimbra Portugal"},{"name":"CNC\u2014Centre for Neuroscience and Cell Biology University of Coimbra Coimbra Portugal"},{"name":"IIIUC\u2014Institute for Interdisciplinary Research University of Coimbra Coimbra Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria In\u00eas","family":"Cristo","sequence":"additional","affiliation":[{"name":"CNC\u2014Centre for Neuroscience and Cell Biology University of Coimbra Coimbra Portugal"},{"name":"IIIUC\u2014Institute for Interdisciplinary Research University of Coimbra Coimbra Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o","family":"Ramalho\u2010Santos","sequence":"additional","affiliation":[{"name":"Department of Life Sciences University of Coimbra Coimbra Portugal"},{"name":"CNC\u2014Centre for Neuroscience and Cell Biology University of Coimbra Coimbra Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5542-7763","authenticated-orcid":false,"given":"Sandra","family":"Amaral","sequence":"additional","affiliation":[{"name":"Department of Life Sciences University of Coimbra Coimbra Portugal"},{"name":"CNC\u2014Centre for Neuroscience and Cell Biology University of Coimbra Coimbra Portugal"},{"name":"IIIUC\u2014Institute for Interdisciplinary Research University of Coimbra Coimbra Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,7,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2018.03.012"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.20471\/acc.2019.58.03.15"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(20)32667\u20102"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmv016"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12958\u2010015\u20100032\u20101"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.XHA.0000397686.82729.09"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmx021"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.5603\/GP.2016.0095"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2021.4788"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2018.01.029.Major"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.12968\/bjon.2016.25.18.S35"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.13158"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2008.11.012"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.13284"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2019.107498"},{"key":"e_1_2_10_17_1","unstructured":"World Health Organization.Examination and processing of human semen. 2010;Edition V(10). 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