{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T15:03:52Z","timestamp":1791644632512,"version":"4.3.4"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,7,17]],"date-time":"2023-07-17T00:00:00Z","timestamp":1689552000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>A growing number of centers worldwide are preserving testicular tissue (TT) of young boys at risk of fertility loss to preserve their fertility. Data in this regard are scarce and experience sharing is essential to the optimization of the process.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>This report of our 10\u2010year activity of pediatric fertility preservation (FP) has the objective to (1) improve knowledge regarding the feasibility, acceptability, safety, and potential usefulness of the procedure; (2) analyze the impact of chemotherapy on spermatogonia in the cryopreserved TT.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>For this retrospective study of data prospectively recorded, we included all boys under 18 years of age referred to the FP consultation of our academic network between October 2009 and December 2019. Characteristics of patients and cryopreservation of testicular tissue (CTT) were extracted from the clinical database. Univariate and multivariate analyses were used to assess factors associated with the risk of absence of spermatogonia in the TT.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Three hundred and sixty\u2010nine patients (7.2 years; 0.5\u201317.0) were referred to the FP consultation for malignant (70%) or non\u2010malignant (30%) disease, of whom 88% were candidates for CTT, after a previous chemotherapy exposure (78%). The rate of recorded immediate adverse events was 3.5%, with painful episodes dominating. Spermatogonia were detected in the majority of TTs: 91.1% of those exposed to chemotherapy and 92.3% of those not exposed (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.962). In multivariate analysis, the risk of absence of spermatogonia was almost three\u2010fold higher in boys\u00a0&gt;\u00a010 years of age ([OR] 2.74, 95% CI 1.09\u20137.26, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.035) and four\u2010fold higher in boys exposed to alkylating agents prior to CTT ([OR] 4.09, 95% CI 1.32\u201317.94, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.028).<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion\/conclusion<\/jats:title><jats:p>This large series of pediatric FP shows that this procedure is well accepted, feasible, and safe in the short term, strengthening its place in the clinical care pathway of young patients requiring a highly gonadotoxic treatment. Our results demonstrate that CTT post\u2010chemotherapy does not impair the chance to preserve spermatogonia in the TT except when the treatment includes alkylating agents. More data on post\u2010CTT follow\u2010up are still required to ensure the long\u2010term safety and usefulness of the procedure.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.13493","type":"journal-article","created":{"date-parts":[[2023,7,7]],"date-time":"2023-07-07T15:41:42Z","timestamp":1688744502000},"page":"385-395","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["A 10\u2010year experience in testicular tissue cryopreservation for boys under 18 years of age: What can be learned from 350 cases?"],"prefix":"10.1111","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8507-7303","authenticated-orcid":false,"given":"Virginie","family":"Barraud\u2010Lange","sequence":"first","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 Paris France"},{"name":"Department of Reproductive Biology CECOS AP\u2010HP. Center\u2010Universit\u00e9 Paris Cite. Cochin Hospital Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Boissel","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 Paris France"},{"name":"Department of Hematology Adolescents and Young Adults Unit AP\u2010HP. North\u2010Universit\u00e9 Paris Cit\u00e9. Saint\u2010Louis Hospital Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne\u2010Sophie","family":"Gille","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 Paris France"},{"name":"Department of Reproductive Biology CECOS AP\u2010HP. Center\u2010Universit\u00e9 Paris Cite. Cochin Hospital Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camille","family":"Jean","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 Paris France"},{"name":"Department of Reproductive Biology CECOS AP\u2010HP. Center\u2010Universit\u00e9 Paris Cite. Cochin Hospital Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leslie","family":"Sitbon","sequence":"additional","affiliation":[{"name":"Biomega\u2010Bioclinic Department Intercommunal Hospital of Cr\u00e9teil Assisted Reproductive Biology Cr\u00e9teil France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9003-0937","authenticated-orcid":false,"given":"Benoit","family":"Schubert","sequence":"additional","affiliation":[{"name":"Eurofins Biomnis Laboratory Institut Rhonalpin IVF Center Clinique du Val d'Ouest Ecully France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karima","family":"Yakouben","sequence":"additional","affiliation":[{"name":"Department of Pediatric Immunology and Hematology APHP. North\u2010Universit\u00e9 Paris Cit\u00e9. Robert Debr\u00e9 Hospital Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mony","family":"Fahd","sequence":"additional","affiliation":[{"name":"Department of Pediatric Immunology and Hematology APHP. North\u2010Universit\u00e9 Paris Cit\u00e9. Robert Debr\u00e9 Hospital Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthieu","family":"Peycelon","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 Paris France"},{"name":"Department of Pediatric Surgery and Urology Centre de R\u00e9f\u00e9rence des Malformations Rares des Voies Urinaires (MARVU) Inserm UMR 1141 NeuroDev APHP. North\u2010Universit\u00e9 Paris Cit\u00e9. Robert\u2010Debr\u00e9 Hospital Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annabel","family":"Paye\u2010Jaouen","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery and Urology Centre de R\u00e9f\u00e9rence des Malformations Rares des Voies Urinaires (MARVU) Inserm UMR 1141 NeuroDev APHP. North\u2010Universit\u00e9 Paris Cit\u00e9. Robert\u2010Debr\u00e9 Hospital Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3703-2519","authenticated-orcid":false,"given":"C\u00e9line","family":"Chalas","sequence":"additional","affiliation":[{"name":"Department of Reproductive Biology CECOS AP\u2010HP. Center\u2010Universit\u00e9 Paris Cite. Cochin Hospital Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexis","family":"Vanhaesebrouck","sequence":"additional","affiliation":[{"name":"Interdisciplinary Research Institute on Social issues (IRIS) UMR 8156\u2013997 Sorbonne Paris North University Aubervilliers France"},{"name":"Department of Legal and Social Medicine AP\u2010HP Jean\u2010Verdier Hospital Bondy France"},{"name":"Department of Social Epidemiology Sorbonne University INSERM Pierre Louis Institute of Epidemiology and Public Health Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Doz","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 Paris France"},{"name":"Curie Institute, SIREDO Center (Care, Innovation, Research in Pediatric, Adolescent and Young Adult Oncology Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aurore","family":"Surun","sequence":"additional","affiliation":[{"name":"Curie Institute, SIREDO Center (Care, Innovation, Research in Pediatric, Adolescent and Young Adult Oncology Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauriane","family":"Lemelle","sequence":"additional","affiliation":[{"name":"Curie Institute, SIREDO Center (Care, Innovation, Research in Pediatric, Adolescent and Young Adult Oncology Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Sarnacki","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 Paris France"},{"name":"Department of Visceral and Urological Pediatric Surgery AP\u2010HP. 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