{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T23:45:28Z","timestamp":1787874328870,"version":"build-2784847793"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T00:00:00Z","timestamp":1693353600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007194","name":"Wenzhou Municipal Science and Technology Bureau","doi-asserted-by":"publisher","award":["Y20180154"],"award-info":[{"award-number":["Y20180154"]}],"id":[{"id":"10.13039\/501100007194","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Backgrounds<\/jats:title><jats:p>Hepatitis B virus infection could result in male infertility with sperm defects and dysfunction. Sertoli cells are essential for testis function and play a crucial role in spermatogenesis. Sertoli cell death contributes to spermatogenesis impairment, leading to poor sperm quality. Ferroptosis has been implicated as a mechanism of Sertoli cell death. The issue in studying the relationship between hepatitis B virus and Sertoli cell ferroptosis has not yet been addressed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To explore the mechanisms underlying ferroptosis in hepatitis B virus\u2010exposed Sertoli cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Human Sertoli cells were treated in vitro with levels of 25, 50, and 100\u00a0\u03bcg\/mL of hepatitis B virus surface protein (HBs). Cell viability and levels of glutathione, malondialdehyde, cellular ferrous ion (Fe<jats:sup>2+<\/jats:sup>), lipid peroxidation, and <jats:italic>N<\/jats:italic>6\u2010methyladenosine in Sertoli cells were detected. The level of glutathione peroxidase 4, transferrin receptor 1, ferritin heavy chain, tripartite motif (TRIM) 37, methyltransferase like 3, and insulin\u2010like growth factor 2 mRNA binding protein 2 was examined. Cell transfection was carried out to alter expression of ferroptosis\u2010related proteins. qPCR and immunoblotting were performed to measure protein expression level. Immunoprecipitation was applied to determine the protein and protein\u2010RNA interaction. Luminescence analysis was performed to identify the target of methyltransferase like 3.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>HBs exposure triggered ferroptosis featured with increased intracellular Fe<jats:sup>2+<\/jats:sup> ion, reduced cell viability and expression of glutathione peroxidase 4 in Sertoli cells. HBs treatment significantly increased TRIM37 expression, which suppressed glutathione peroxidase 4 expression through ubiquitination. TRIM37 silencing attenuated the effect of HBs exposure\u2010regulated cell viability and ferroptosis. HBs upregulated <jats:italic>N<\/jats:italic>6\u2010methyladenosine modification in TRIM37 3\u2032\u2010UTR by increasing methyltransferase like 3 expression. The binding of <jats:italic>N<\/jats:italic>6\u2010methyladenosine reader insulin\u2010like growth factor 2 mRNA binding protein 2 and TRIM37 3\u2032\u2010UTR enhanced the stability of TRIM37 mRNA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>HBs can decrease human Sertoli cell viability by promoting ferroptosis induced by the loss of glutathione peroxidase 4 activity through TRIM37\u2010mediated ubiquitination of glutathione peroxidase 4. The findings highlight the role of TRIM37\/glutathione peroxidase 4 signaling responsible for ferroptosis regulation in hepatitis B virus\u2010infected Sertoli cells.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.13520","type":"journal-article","created":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T07:35:35Z","timestamp":1693380935000},"page":"643-654","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The role of hepatitis B virus surface protein in inducing Sertoli cell ferroptosis"],"prefix":"10.1111","volume":"12","author":[{"given":"Chengshuang","family":"Pan","sequence":"first","affiliation":[{"name":"Reproductive Medicine Center The First Affiliated Hospital of Wenzhou Medical University  Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangbin","family":"Kong","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center The First Affiliated Hospital of 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