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We generated knockout mouse lines lacking seven candidate proteins by the CRISPR\/Cas9 system and conducted detailed analyses on the fecundity of the knockout males, as well as their testis appearance and weight, testis and epididymis histology, and sperm motility and morphology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Through the in\u2010silico screen, we identified 4932438H23Rik, A disintegrin and metalloproteinase domain\u2010containing protein 29, SAYSvFN domain\u2010containing protein 1, Sel\u20101 suppressor of lin\u201012\u2010like 2 (<jats:italic>C. elegans<\/jats:italic>), Testis\u2010expressed protein 2, Transmembrane and immunoglobulin domain\u2010containing 3, and Zinc and ring finger 4. Phenotypic analyses unveiled that the knockout males showed normal testis gross appearance, normal testis and epididymis histology, and normal sperm morphology and motility. Fertility tests further indicated that the knockout male mice could sire pups with normal litter sizes when paired with wild\u2010type females.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion and conclusion<\/jats:title><jats:p>These findings suggest that these seven proteins are individually dispensable for male reproduction and fertilization. Future studies are warranted to devise advanced in\u2010silico screening approaches that permit effective identification of gamete fusion\u2010required sperm proteins.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.13564","type":"journal-article","created":{"date-parts":[[2023,12,12]],"date-time":"2023-12-12T22:35:39Z","timestamp":1702420539000},"page":"1251-1260","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["CRISPR\/Cas9\u2010mediated genome editing reveals seven testis\u2010enriched transmembrane glycoproteins dispensable for male fertility in mice"],"prefix":"10.1111","volume":"13","author":[{"given":"Yo","family":"Ogawa","sequence":"first","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences Osaka University  Suita Japan"},{"name":"Department of Experimental Genome Research, Research Institute for Microbial Diseases Osaka University  Suita 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Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsin\u2010Yi","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Experimental Genome Research, Research Institute for Microbial Diseases Osaka University  Suita Japan"},{"name":"Graduate School of Medicine Osaka University  Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9859-6217","authenticated-orcid":false,"given":"Masahito","family":"Ikawa","sequence":"additional","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences Osaka University  Suita Japan"},{"name":"Department of Experimental Genome Research, Research Institute for Microbial Diseases Osaka University  Suita Japan"},{"name":"Graduate School of Medicine Osaka University  Suita Japan"},{"name":"The Institute of Medical Science The University of Tokyo  Tokyo 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