{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T01:46:29Z","timestamp":1789004789341,"version":"build-2803163510"},"reference-count":60,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Most commerce of equine seminal doses is carried out using commercial extenders under refrigeration at 5\u00b0C.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To determine if 10\u00a0m<jats:sc>m<\/jats:sc> pyruvate in a 67\u00a0m<jats:sc>m<\/jats:sc> glucose extender and storage at 22\u00b0C could be the basis of an alternative storage method to cooling to 5\u00b0C.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>Stallion ejaculates were extendedin: INRA96 (67\u00a0m<jats:sc>m<\/jats:sc> glucose, non\u2010pyruvate control), modified Tyrode's (67\u00a0m<jats:sc>m<\/jats:sc> glucose\u201310\u00a0m<jats:sc>m<\/jats:sc> pyruvate), supplemented with 0, 10, 50, and 100\u00a0\u03bcM itaconate. As itaconate was vehiculated in DMSO, a control vehicle was also included. Sperm motility, viability, mitochondrial membrane potential, and production of reactive oxygen species were measured after collection and again after 48 and 96\u00a0h of storage at 22\u00b0C. To disclose molecular metabolic changes, spermatozoa were incubated up to 3\u00a0h in modified Tyrode's 67\u00a0m<jats:sc>m<\/jats:sc> glucose\u201310\u00a0m<jats:sc>m<\/jats:sc> pyruvate and modified Tyrode's 67\u00a0m<jats:sc>m<\/jats:sc> glucose, and metabolic analysis conducted.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>After 96\u00a0h of storage aliquots stored in the control, INRA96 had a very poor total motility of 5.6%\u00a0\u00b1\u00a02.3%, while in the 67\u00a0m<jats:sc>m<\/jats:sc> glucose\u201310\u00a0m<jats:sc>m<\/jats:sc> pyruvate\/10\u00a0\u03bc<jats:sc>m<\/jats:sc> itaconate extender, total motility was 34.7%\u00a0\u00b1\u00a03.8% (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.0066). After 96\u00a0h, viability was better in most pyruvate\u2010based media, and the mitochondrial membrane potential in spermatozoa extended in INRA96 was relatively lower (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.0001). Metabolomics revealed that in the spermatozoa incubated in the high pyruvate media, there was an increase in the relative amounts of <jats:italic>NAD<jats:sup>+<\/jats:sup>, pyruvate, lactate<\/jats:italic>, and <jats:italic>ATP<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion and conclusions<\/jats:title><jats:p>Aliquots stored in a 67\u00a0m<jats:sc>m<\/jats:sc> glucose\u201310\u00a0m<jats:sc>m<\/jats:sc> pyruvate\u2010based medium supplemented with 10\u00a0\u03bcM itaconate, maintained a 35% total motility after 96\u00a0h of storage at 22\u00b0C, which is considered the minimum acceptable motility for commercialization. Improvements may be related to the conversion of pyruvate to lactate and regeneration of NAD<jats:sup>+<\/jats:sup>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.13565","type":"journal-article","created":{"date-parts":[[2023,12,2]],"date-time":"2023-12-02T17:01:10Z","timestamp":1701536470000},"page":"1170-1185","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Aging of stallion spermatozoa stored in vitro is delayed at 22\u00b0C using a 67\u00a0m<scp>m<\/scp> glucose\u201310\u00a0m<scp>m<\/scp> pyruvate\u2010based media"],"prefix":"10.1111","volume":"12","author":[{"given":"Laura","family":"Becerro\u2010Rey","sequence":"first","affiliation":[{"name":"Laboratory of Equine Reproduction and Equine Spermatology Veterinary Teaching Hospital, Universidad de Extremadura C\u00e1ceres Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco Eduardo","family":"Mart\u00edn\u2010Cano","sequence":"additional","affiliation":[{"name":"Laboratory of Equine Reproduction and Equine Spermatology Veterinary Teaching Hospital, Universidad de Extremadura C\u00e1ceres Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina Ortega","family":"Ferrusola","sequence":"additional","affiliation":[{"name":"Laboratory of Equine Reproduction and Equine Spermatology Veterinary Teaching Hospital, Universidad de Extremadura C\u00e1ceres Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heriberto","family":"Rodr\u00edguez\u2010Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Clinical Sciences (BKV) Obstetrics &amp; Gynaecology (BKH) University of Link\u00f6ping  Link\u00f6ping Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gemma","family":"Gaitskell\u2010Phillips","sequence":"additional","affiliation":[{"name":"Laboratory of Equine Reproduction and Equine Spermatology Veterinary Teaching Hospital, Universidad de Extremadura C\u00e1ceres Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"da Silva\u2010\u00c1lvarez","sequence":"additional","affiliation":[{"name":"Laboratory of Equine Reproduction and Equine Spermatology Veterinary Teaching Hospital, Universidad de Extremadura C\u00e1ceres Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Silva\u2010Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Facility of Innovation and Analysis in Animal Source Foodstuffs Universidad de Extremadura C\u00e1ceres Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Cruz","family":"Gil","sequence":"additional","affiliation":[{"name":"Laboratory of Equine Reproduction and Equine Spermatology Veterinary Teaching Hospital, Universidad de Extremadura C\u00e1ceres Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1311-2947","authenticated-orcid":false,"given":"Fernando J.","family":"Pe\u00f1a","sequence":"additional","affiliation":[{"name":"Laboratory of Equine Reproduction and Equine Spermatology Veterinary Teaching Hospital, Universidad de Extremadura C\u00e1ceres Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2022.107088"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jevs.2016.06.006"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093\u2010691x(87)90288\u20103"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0378\u20104320(01)00155\u20105"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093\u2010691x(95)00290\u2010o"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jevs.2021.103801"},{"issue":"56","key":"e_1_2_9_8_1","first-page":"69","article-title":"Preservation of stallion sperm quality by native phosphocaseinate: a direct or indirect effect?","author":"Batellier F","year":"2000","journal-title":"J Reprod Fertil 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