{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T03:12:05Z","timestamp":1776395525374,"version":"3.51.2"},"reference-count":44,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,6,24]],"date-time":"2024-06-24T00:00:00Z","timestamp":1719187200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2021\/02341\u20106"],"award-info":[{"award-number":["2021\/02341\u20106"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Peyronie's disease is characterized by the formation of fibrotic plaques in the penile tunica albuginea. Effective treatments are limited, warranting the investigation of new promising therapies, such as the application of microRNAs that regulate fibrosis\u2010related genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>We aimed to investigate the therapeutic potential of mimicking microRNA\u201029b in a fibrin\u2010induced rat model of Peyronie's disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material\/methods<\/jats:title><jats:p>The study was designed in two phases. To establish an optimal Peyronie's disease model, rats received either human fibrin and thrombin or saline solutions into the tunica albuginea on days 0 and 5. The animal model validation was done through expression and histopathological analyses, the latest by an experienced uropathologist. After validation, we performed microRNA\u201029b treatment on days 14, 21, and 28 of the study. This phase had control (normal saline) and scramble (microRNA scramble) groups. The mid\u2010penile shaft was removed on day 30 for histological examination and molecular analyses in both study stages.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The control group displayed typical tunica albuginea histologic architecture in the animal model validation. In Peyronie's disease group, the Hematoxylin and eosin and Masson Trichrome staining methods demonstrated an interstitial inflammatory process with concomitant dense fibrotic plaques as well as disarrangement of collagen fibers. Additionally, we found out that reduced microRNA\u201029b (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.05) was associated with significantly increased COL1A1 and transforming growth factor \u03b21 genes and proteins (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a00.05) in the Peyronie's disease group. After treatment with mimic microRNA\u201029b stimulation, the Hematoxylin &amp; eosin and Masson Trichrome staining revealed a discrete and less dense fibrotic plaque. This result was associated with significantly decreasing expression of COL1A1, COL3A1, and transforming growth factor \u03b21 genes and proteins (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>The fibrin\u2010induced animal model showed significant histopathological and molecular changes compared to the Control group, suggesting that our model was appropriate. Previous findings have shown that increased expression of microRNA\u201029b was associated with decreased pathological fibrosis. In the present study, treatment with microRNA\u201029b decreased the gene and protein expression of collagens and transforming growth factor \u03b21. This study reveals the therapeutic potential for Peyronie's disease involving molecular targets.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>MicroRNA\u201029b application on the rat's tunica albuginea attenuated fibrosis, arising as a novel potential strategy for Peyronie's disease management.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.13672","type":"journal-article","created":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T04:44:56Z","timestamp":1719290696000},"page":"396-406","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["MicroRNA\u201029b attenuates fibrosis in a rat model of Peyronie's disease"],"prefix":"10.1111","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5887-5617","authenticated-orcid":false,"given":"Patr\u00edcia","family":"Candido","sequence":"first","affiliation":[{"name":"Urology Department Laboratory of Medical Investigation (LIM55) Faculdade de Medicina da Universidade de S\u00e3o Paulo (FMUSP)  Sao Paulo Brazil"},{"name":"Moriah Institute of Science and Education (MISE) Hospital Moriah  Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruan","family":"Pimenta","sequence":"additional","affiliation":[{"name":"Urology Department Laboratory of Medical Investigation (LIM55) Faculdade de Medicina da Universidade de S\u00e3o Paulo (FMUSP)  Sao Paulo Brazil"},{"name":"Department of Immunology and Immunotherapy and Tisch Cancer Institute Precision Immunology Institute Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feres Camargo","family":"Maluf","sequence":"additional","affiliation":[{"name":"Urology Department Laboratory of Medical Investigation (LIM55) Faculdade de Medicina da Universidade de S\u00e3o Paulo (FMUSP)  Sao Paulo Brazil"},{"name":"Department of Urology University of California San Francisco  San Francisco 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dos","family":"Santos","sequence":"additional","affiliation":[{"name":"Urology Department Laboratory of Medical Investigation (LIM55) Faculdade de Medicina da Universidade de S\u00e3o Paulo (FMUSP)  Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iran A.","family":"Silva","sequence":"additional","affiliation":[{"name":"Urology Department Laboratory of Medical Investigation (LIM55) Faculdade de Medicina da Universidade de S\u00e3o Paulo (FMUSP)  Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Division of Urology Hospital das Cl\u00ednicas da Faculdade de Medicina da Universidade de S\u00e3o Paulo (HCFMUSP)  Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge","family":"Hallak","sequence":"additional","affiliation":[{"name":"Division of Urology Hospital das Cl\u00ednicas da Faculdade de Medicina da 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