{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T21:33:58Z","timestamp":1768253638650,"version":"3.49.0"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,7,17]],"date-time":"2024-07-17T00:00:00Z","timestamp":1721174400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Commercial products currently available for sperm selection utilizing hyaluronic acid (HA) binding prior to intracytoplasmic sperm injection (ICSI) are widely used but have some disadvantages. To potentially circumvent these limitations, we compared ICSI using a self\u2010made hyaluronic acid (smHA) reagent with ICSI using SpermSlow.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The binding of the reagents to spermatozoa on plastic\u2010 or glass\u2010bottom dishes was quantitated using spermatozoa that were isolated by density\u2010gradient centrifugation and swim\u2010up procedures (<jats:italic>N\u00a0<\/jats:italic>=\u00a010\/group). Additionally, we investigated the relationship between the HA reagent used prior to ICSI and clinical outcomes after assisted reproduction with HA\u2010ICSI (<jats:italic>N\u00a0<\/jats:italic>=\u00a081).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The smHA reagent exhibited extremely stable binding to human spermatozoa. The binding time of spermatozoa was significantly longer in the smHA reagent than in SpermSlow on both plastic and glass dishes (plastic: 60.0\u00a0\u00b1\u00a00.0 min vs. 2.7\u00a0\u00b1\u00a05.9 min, <jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.001; glass: 60.0\u00a0\u00b1\u00a00.0 min vs. 2.5\u00a0\u00b1\u00a01.8 min, <jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.001). There were no significant differences in the normal fertilization rate between HA\u2010ICSI with the smHA reagent (128\/160, 80.0%) and HA\u2010ICSI with SpermSlow (171\/231, 74.0%, <jats:italic>P\u00a0<\/jats:italic>=\u00a00.184). The frequency of the blastocyst development from the HA\u2010ICSI\u2010derived zygote was significantly higher with the smHA reagent (74\/101, 73.3%) than with SpermSlow (76\/131, 58.0%, <jats:italic>P\u00a0<\/jats:italic>=\u00a00.019). The rates of biochemical pregnancy, clinical pregnancy, fetal heart movement, live birth, and miscarriage were not significantly different between HA\u2010ICSI with the smHA reagent and HA\u2010ICSI with SpermSlow.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The blastulation rate was higher for HA\u2010ICSI with the smHA reagent as compared with SpermSlow. Clinical outcomes, excluding blastulation, after HA\u2010ICSI were the same using smHA reagent and using SpermSlow. Spermatozoa binding to the smHA reagent was not attenuated over a 60\u2010min time course. In conclusion, this reagent may shorten and simplify HA\u2010ICSI procedures because smHA can be used with any dish material, making it easier to observe the spindle or assess intracytoplasmic morphology.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.13683","type":"journal-article","created":{"date-parts":[[2024,7,17]],"date-time":"2024-07-17T11:39:17Z","timestamp":1721216357000},"page":"555-563","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A hyaluronic acid\u2010containing reagent compatible with glass\u2010bottom dishes and capable of sustained binding of human spermatozoa"],"prefix":"10.1111","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9605-2379","authenticated-orcid":false,"given":"Taketo","family":"Inoue","sequence":"first","affiliation":[{"name":"Umeda Fertility Clinic, 3\u201017\u20106, Toyosaki, Kita\u2010ku  Osaka Osaka Japan"},{"name":"Department of Emergency Disaster and Critical 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