{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T16:50:41Z","timestamp":1774457441105,"version":"3.50.1"},"reference-count":58,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T00:00:00Z","timestamp":1725926400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Testicular torsion\/detorsion can cause testis loss and infertility. Aloperine is a major active alkaloid extracted from <jats:italic>Sophora alopecuroides<\/jats:italic> Linn. It has been shown to have organ\u2010protective effects. However, the effects of aloperine on the testis and its underlying mechanisms remain unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>This study investigated the effect of aloperine on testicular torsion\/detorsion injury in rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Male Sprague\u2010Dawley rats were randomized to the sham\u2010operated (sham), testicular I\/R (TI\/R), or aloperine preconditioning (ALOPre) or postconditioning (ALOPost) groups. All rats except for the sham\u2010operated rats were subjected to 3\u00a0h of right spermatic cord torsion (720\u00b0, clockwise), followed by 3\u00a0h of detorsion. Aloperine (10\u00a0mg\/kg) was intravenously administered before testicular torsion (ALOPre) or at the onset of testicular detorsion (ALOPost). The therapeutic efficacy of aloperine was evaluated by histological analysis, oxidative stress evaluation, inflammatory response examination, apoptosis analysis, protein analysis, and immunohistological assessment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared with TI\/R, aloperine protected both the ipsilateral and contralateral testes against unilateral testicular I\/R, as evidenced by a reduced testicular weight to body weight (TW\/BW) ratio (ALOPre: <jats:italic>p\u00a0<\/jats:italic>=\u00a00.0037; ALOPost: <jats:italic>p\u00a0<\/jats:italic>=\u00a00.0021) and volume (ALOPre: <jats:italic>p\u00a0<\/jats:italic>=\u00a00.0020; ALOPost: <jats:italic>p\u00a0<\/jats:italic>=\u00a00.0009), less structural damage with better Johnsen (ALOPre: <jats:italic>p\u00a0<\/jats:italic>=\u00a00.0013; ALOPost: <jats:italic>p\u00a0<\/jats:italic>=\u00a00.0021), and Cosentino scores (ALOPre: <jats:italic>p\u00a0<\/jats:italic>&lt;\u00a00.0001; ALOPost: <jats:italic>p\u00a0<\/jats:italic>&lt;\u00a00.0001), increased mean seminiferous tubule diameter and mean seminiferous tubule epithelial height, decreased testicular apoptosis, and less oxidative stress and inflammatory response. In addition, aloperine significantly stimulated the phosphorylation of signal transducer and activator of transcription (STAT)\u20103 in the ipsilateral testes following detorsion. Administration of Ag490 suppressed STAT\u20103 phosphorylation, thereby abrogating the protective effects exerted by aloperine on the ipsilateral testis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion and Conclusion<\/jats:title><jats:p>Aloperine has a strong testicular protective effect on the ipsilateral and contralateral testes after testicular torsion\/detorsion. This aloperine\u2010induced ipsilateral testicular protection is mediated via the STAT\u20103 signaling pathway.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.13750","type":"journal-article","created":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T08:43:08Z","timestamp":1725957788000},"page":"934-954","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Aloperine protects the testis against testicular ischemia\/reperfusion injury in rats"],"prefix":"10.1111","volume":"13","author":[{"given":"Shichao","family":"Wei","sequence":"first","affiliation":[{"name":"Department of Anesthesiology, West China Hospital Sichuan University  Chengdu Sichuan China"},{"name":"Laboratory of Anesthesia and Critical Care Medicine, National\u2010Local Joint Engineering Research Centre of Translational Medicine of Anesthesiology, West China Hospital Sichuan University  Chengdu Sichuan 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