{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T16:04:11Z","timestamp":1791389051874,"version":"4.3.3"},"reference-count":20,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,10,17]],"date-time":"2024-10-17T00:00:00Z","timestamp":1729123200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To compare clinical outcomes using short and long co\u2010incubation protocols in sibling oocytes based on embryo morphokinetic outcomes measured by time\u2010lapse incubator with stratification based on a woman's age and sperm quality.<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>Our study included 72 cycles with\u00a0&gt;6 oocytes retrieved. Sibling oocytes were distributed for two parallel protocols: short (3\u00a0h; <jats:italic>n<\/jats:italic>\u00a0=\u00a0421) or long (16\u201320\u00a0h; <jats:italic>n<\/jats:italic>\u00a0=\u00a0434) insemination, using the same amount of spermatozoa from the same prepared sample. Oocytes were then washed and incubated for 5 days. Time\u2010lapse annotations of embryos were performed by experienced embryologists and artificial intelligence\u2010based Known Implantation Data scores for day 3 and day 5 were calculated with EmbryoScope software.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Short\u2010insemination group exhibited a higher blastulation rate, better morphokinetic indicators, and higher Known Implantation Data scores on day 3 and day 5 of the utilized embryos. However, the fertilization rate and clinical pregnancy rate per embryo transfer did not differ between experimental groups. A higher rate of abnormal fertilization (&gt;2 pronuclei) after long insemination was recorded in women under 35 years old or with a total motile sperm count above 5 million and above 40% motility after preparation. A higher rate of usable embryos was observed after short insemination with a total motile sperm count above 30 million before preparation or 5 million and over 40% motility after preparation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results suggest that a short insemination protocol results in better embryo quality and should be considered as a favorable protocol, especially in young female patients or male patients with high sperm quality.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.13781","type":"journal-article","created":{"date-parts":[[2024,10,17]],"date-time":"2024-10-17T03:03:44Z","timestamp":1729134224000},"page":"1402-1407","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Short insemination during conventional in vitro fertilization increases embryo 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