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Maximizing the utility of captive research marmosets, including genetically manipulated animals, will require the use of assisted reproductive techniques (ART) including manipulation, storage, and sharing of marmoset sperm. Here, we identify characteristics of high\u2010quality semen samples and validate a simple method for selecting high\u2010quality sperm.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Computer\u2010assisted sperm analysis (CASA) was used to evaluate sperm quality in semen samples collected from 44 marmosets and assessed the use of the swim\u2010up method for the selection of high\u2010quality sperm was also tested in half the samples as a potential means to optimize in vitro fertilization or intrauterine insemination.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>For each reference parameter, samples at or below the 5th percentile were categorized as abnormal sperm, while those above the 5th percentile were considered to be normal. Among normal samples, those at or above the 50th percentile were categorized as high\u2010quality. High\u2010quality semen samples exhibited the following characteristics: semen volume \u2265 30\u00a0\u00b5L; sperm count \u2265 10<jats:sup>7<\/jats:sup>\/ejaculate; total motility \u2265 35%; and normal morphology \u2265 5%. Sperm isolated by swim\u2010up exhibited superior sperm progressive motility (19.7%\u00a0\u00b1\u00a04.5\u00a0vs. 5.6%\u00a0\u00b1\u00a02.1; <jats:italic>p<\/jats:italic>\u00a0=\u00a00.01) and normal morphology (13.1\u00a0\u00b1\u00a01.59\u00a0vs. 7.65\u00a0\u00b1\u00a01.1; <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001) compared with unselected sperm.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study defines robust, statistically supported reference values for evaluating marmoset semen samples to assist with the identification of optimal sperm donors and the selection of high\u2010quality sperm samples for assisted reproduction. Ultimately, these reference values combined with a validated selection method will contribute to consistent standards for the international sharing of genetically diverse and\/or gene\u2010edited marmoset sperm for research and reproduction.<\/jats:p><\/jats:sec>","DOI":"10.1111\/andr.13782","type":"journal-article","created":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T11:04:58Z","timestamp":1729595098000},"page":"1530-1540","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Benchmarks defining high\u2010quality sperm in the common marmoset (<i>Callithrix jacchus<\/i>)"],"prefix":"10.1111","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4388-4566","authenticated-orcid":false,"given":"Niloofar","family":"Sadeghi","sequence":"first","affiliation":[{"name":"Department of Neuroscience Developmental and Regenerative Biology The University of Texas at San Antonio  San Antonio Texas 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Neuroscience Developmental and Regenerative Biology The University of Texas at San Antonio  San Antonio Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0503-3616","authenticated-orcid":false,"given":"Brian P.","family":"Hermann","sequence":"additional","affiliation":[{"name":"Department of Neuroscience Developmental and Regenerative Biology The University of Texas at San Antonio  San Antonio Texas 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