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Protein kinase A (pPKA) and PKC (pPKC) substrates, pERK1\/2, G\/F\u2010actin were determined by Western blotting, activation of RAC\u20101 by pull\u2010down assay, F\u2010actin and acrosomal exocytosis by fluorescence microscopy, sperm motility by optic microscopy and computer\u2010aided sperm analysis and fertilizing ability by in vitro fertilization (IVF).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We detected GPR55 in spermatozoa at T0, after incubation in non\u2010capacitating and capacitating (presence of AEA) conditions and upon release from oviductal epithelia. AEA induced an increase in pPKA and pPKC, while CID16020046 (CID), selective GPR55 antagonist, prevented this effect. Incubation with H89, PKA inhibitor, significantly decreased pPKC, while G\u00f66983, a PKC inhibitor, partially reduced pPKA. pPKA remained elevated at 15\u2010 and 45\u2010min incubation, while pPKC decreased at 15 and increased at 45\u00a0min. CID prevented pPKC increase at 5 and 45\u00a0min and decreased pPKA at 45\u00a0min. RAC\u20101 and F\u2010actin increase induced by AEA was prevented by CID. Variations in two progressive motility kinematic parameters were observed with AEA and\/or CID. Sperm pretreatment with AEA increased the rate of cleaved embryos and CID prevented this effect.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>We demonstrated that GPR55 activation by AEA induces time\u2010dependent signaling pathways involving pPKA and pPKC during bovine sperm capacitation. AEA regulates actin polymerization through GPR55 activation, suggesting the receptor participates in cytoskeleton remodeling, and yielded higher IVF rates. Also, sperm pre\u2010incubation with molecules like AEA involved in capacitation could improve the embryo development.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>We have demonstrated GPR55 presence in bovine spermatozoa. The regulation of PKA and PKC and of molecules associated with cytoskeletal dynamics, such as RAC\u20101 and actin, by GPR55 is closely related to sperm motility and acrosomal exocytosis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/andr.13823","type":"journal-article","created":{"date-parts":[[2025,1,3]],"date-time":"2025-01-03T06:34:35Z","timestamp":1735886075000},"page":"1848-1865","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Role of GPR55 receptor in bovine sperm capacitation"],"prefix":"10.1111","volume":"13","author":[{"given":"Raquel","family":"Lottero\u2010Leconte","sequence":"first","affiliation":[{"name":"Centro de Estudios Farmacol\u00f3gicos y Bot\u00e1nicos (CEFYBO) Facultad de Medicina\u2010Universidad de Buenos Aires (UBA\/CONICET)  Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Lara","sequence":"additional","affiliation":[{"name":"Centro de Estudios Farmacol\u00f3gicos y Bot\u00e1nicos (CEFYBO) Facultad de Medicina\u2010Universidad de Buenos Aires (UBA\/CONICET)  Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Plaza","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Veterinarias, Instituto de Investigaci\u00f3n y Tecnolog\u00eda en Reproducci\u00f3n Animal (INITRA) Universidad de Buenos Aires  Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6312-396X","authenticated-orcid":false,"given":"Camila","family":"Arroyo\u2010Salvo","sequence":"additional","affiliation":[{"name":"Centro de Estudios Farmacol\u00f3gicos y Bot\u00e1nicos (CEFYBO) Facultad de Medicina\u2010Universidad de Buenos Aires (UBA\/CONICET)  Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Eugenia","family":"Bogetti","sequence":"additional","affiliation":[{"name":"Centro de Estudios Farmacol\u00f3gicos y Bot\u00e1nicos (CEFYBO) Facultad de Medicina\u2010Universidad de Buenos Aires (UBA\/CONICET)  Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amada Eugenia Ynsaurralde","family":"Rivolta","sequence":"additional","affiliation":[{"name":"Laboratorio de Biotecnolog\u00eda de la Reproducci\u00f3n. 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