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Defects in chromosome alignment result in chromosome instability, birth defects, and infertility. Kinesin\u20107 CENP\u2010E mediates kinetochore\u2013microtubule capture, chromosome alignment, and spindle assembly checkpoint in somatic cells, however, mechanisms of CENP\u2010E in germ cells remain poorly understood.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study aimed to explore the functions of CENP\u2010E in the proliferation and self\u2010renewal of spermatogonia.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and methods<\/jats:title>\n                    <jats:p>\n                      A\n                      <jats:italic>CENP\u2010E<\/jats:italic>\n                      heterozygous knockout strain was established in C57BL\/6J mice using the CRISPR\/Cas9 and Cre\/LoxP system. Hematoxylin\u2013eosin staining was performed to study the histology. The inhibition of CENP\u2010E in the GC\u20101 spg cells was performed using the specific inhibitor GSK923295. The expression and localization of spermatogonial marker proteins were determined by immunofluorescence using confocal microscopy in the control and\n                      <jats:italic>\n                        CENP\u2010E\n                        <jats:sup>+\/\u2212<\/jats:sup>\n                      <\/jats:italic>\n                      heterozygous mouse testes. The protein expression level was analyzed using Western blot. The cell\u2010cycle and apoptosis assay were measured using flow cytometry. In addition, karyotype analysis was performed using hypotonic preparation and chromosome spreading.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Here, we reveal that\n                      <jats:italic>CENP\u2010E<\/jats:italic>\n                      haploinsufficiency results in chromosome misalignment, spindle disorganization, and metaphase arrest in spermatogonia, which leads to the loss of spermatogonia, chromosomal instability, and spermatogenic disorders. Notably, CENP\u2010E ablation leads to the activation of spindle assembly checkpoint and aneuploidy, which impairs the proliferation and self\u2010renewal of spermatogonia.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion and conclusion<\/jats:title>\n                    <jats:p>CENP\u2010E depletion disrupts the recruitment of key checkpoint proteins, including BubR1, Bub1, KIF2C, and Aurora B, indicating a causal relationship between chromosome misalignment and spindle assembly checkpoint activation in spermatogonia. Our findings demonstrate that CENP\u2010E regulates kinetochore\u2013microtubule attachment, chromosome alignment, and spindle assembly checkpoint in spermatogonia.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/andr.13834","type":"journal-article","created":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T02:55:13Z","timestamp":1735613713000},"page":"1816-1834","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<i>CENP\u2010E<\/i>\n                    haploinsufficiency causes chromosome misalignment and spindle assembly checkpoint activation in the spermatogonia"],"prefix":"10.1111","volume":"13","author":[{"given":"Jie","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Cell Biology and Genetics The School of Basic Medical Sciences Fujian Medical University  Fuzhou Fujian China"},{"name":"Key Laboratory of Stem Cell Engineering and Regenerative Medicine Fujian Province 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