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The effect of mechanical removal of the epithelium and the effects of pre\u2010incubation of RISV strips with N\n                      <jats:sup>\u03c9<\/jats:sup>\n                      \u2010nitro\u2010L\u2010arginine methyl ester (L\u2010NAME; in combination or not with L\u2010arginine), tetrodotoxin (TTX), GKT137831, and hydrogen peroxide (H\n                      <jats:sub>2<\/jats:sub>\n                      O\n                      <jats:sub>2<\/jats:sub>\n                      ) on 6\u2010ND release were evaluated. For functional studies, RISV strips were mounted in an organ bath and tied to an isometric force transducer. The expressions of tyrosine hydroxylase and endothelial nitric oxide synthase (eNOS, type III NOS) were investigated by immunohistochemistry and\/or fluorescence in situ hybridization (FISH).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      6\u2010ND was the major released catecholamine from RISV strips compared with noradrenaline, adrenaline, and dopamine. Epithelium removal significantly reduced the release of 6\u2010ND, noradrenaline and dopamine. In RISV strips obtained from animals chronically treated with L\u2010NAME, the 6\u2010ND release significantly reduced. Pre\u2010incubation with L\u2010NAME reduced 6\u2010ND release, which was partly restored by co\u2010incubation with L\u2010arginine. Pre\u2010incubation with TTX had no effect on the release of any catecholamine, whereas GKT137831 and H\n                      <jats:sub>2<\/jats:sub>\n                      O\n                      <jats:sub>2<\/jats:sub>\n                      significantly increased 6\u2010ND release. All catecholamines produced concentration\u2010dependent RISV contractions, but 6\u2010ND was approximately 30\u00d7 less potent than the others. 6\u2010ND (0.1 nM) significantly potentiated noradrenaline\u2010, adrenaline\u2010, and dopamine\u2010induced contractions, with such potentiations inhibited by TTX. Immunohistochemistry and FISH assays in RISV tissues identified tyrosine hydroxylase in epithelial cells and eNOS expression in both epithelial and endothelial cells.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion and conclusion<\/jats:title>\n                    <jats:p>This is the first demonstration that epithelial\u2010derived 6\u2010ND modulates rat seminal vesicle contractility.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/andr.13836","type":"journal-article","created":{"date-parts":[[2025,1,9]],"date-time":"2025-01-09T01:24:48Z","timestamp":1736385888000},"page":"2304-2317","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["6\u2010Nitrodopamine is an endogenous mediator of rat seminal vesicles 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