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To explore the protective effects of Icariside II (ICA II) on human cavernous endothelial cells, HCECs were isolated from non\u2010diabetic human donors, cultured under high glucose (HG) conditions and treated with ICA II. The cell apoptosis and proliferation, expression of Ki67 and Erk1\/2, antioxidant capacity, and expression of Akt and <jats:styled-content style=\"fixed-case\">eNOS<\/jats:styled-content> were examined. Changes in cell apoptosis and proliferation indicated that HG treatment inhibited HCEC proliferation with lower percentage of Ki67\u2010positive cells and lower expression and phosphorylation of Erk1\/2. Furthermore, the total antioxidant capacity (T\u2010AOC) of HCECs was reduced under HG conditions. In line with these findings, both expression and phosphorylation of Akt as well as <jats:styled-content style=\"fixed-case\">eNOS<\/jats:styled-content> was down regulated after HG treatment. The reduction in proliferative capacity, p\u2010Erk1\/2, p\u2010Akt, and p\u2010<jats:styled-content style=\"fixed-case\">eNOS<\/jats:styled-content> were partially prevented by ICA II in a concentration\u2010dependent manner. The protective effects of ICA II rescued HCEC from injury and dysfunction induced by HG in vitro. ICA II may be a candidate for prevention of the development of diabetic erectile dysfunction.<\/jats:p>","DOI":"10.1111\/andr.303","type":"journal-article","created":{"date-parts":[[2015,1,8]],"date-time":"2015-01-08T06:05:13Z","timestamp":1420697113000},"page":"408-416","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Icariside II prevents high\u2010glucose\u2010induced injury on human cavernous endothelial cells through Akt\u2010<scp>eNOS<\/scp> signaling pathway"],"prefix":"10.1111","volume":"3","author":[{"given":"H.","family":"Li","sequence":"first","affiliation":[{"name":"Molecular Biology Laboratory of Andrology Center Peking University First Hospital Peking University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Xu","sequence":"additional","affiliation":[{"name":"Molecular Biology Laboratory of Andrology Center Peking University First Hospital Peking University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Guan","sequence":"additional","affiliation":[{"name":"Molecular Biology Laboratory of Andrology Center Peking University First Hospital Peking University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Matheu","sequence":"additional","affiliation":[{"name":"Diabetes Center University of California San Francisco CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Lei","sequence":"additional","affiliation":[{"name":"Molecular Biology Laboratory of Andrology Center Peking University First Hospital Peking University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Tian","sequence":"additional","affiliation":[{"name":"Molecular Biology Laboratory of Andrology Center Peking University First Hospital Peking University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.","family":"Gao","sequence":"additional","affiliation":[{"name":"Molecular Biology Laboratory of Andrology Center Peking University First Hospital Peking University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Urology University of California San Francisco CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Guo","sequence":"additional","affiliation":[{"name":"Molecular Biology Laboratory of Andrology Center Peking University First Hospital Peking University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.","family":"Xin","sequence":"additional","affiliation":[{"name":"Molecular Biology Laboratory of Andrology Center Peking University First Hospital Peking University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Song","sequence":"additional","affiliation":[{"name":"Molecular Biology Laboratory of Andrology Center Peking University First Hospital Peking University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,1,8]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.111.228999"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc11-s239"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.2337\/diab.44.11.1323"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02628462"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/ijir.2012.30"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ijir.2012.4"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1743-6109.2008.01183.x"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2008.09.001"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvq119"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0039430"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1530\/JOE-12-0120"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules16119234"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.107.155960"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2011.10.115"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms13055628"},{"key":"e_1_2_9_17_1","doi-asserted-by":"crossref","first-page":"621","DOI":"10.2337\/diab.34.7.621","article-title":"Glucose toxicity for human endothelial cells in culture. 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