{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T16:56:54Z","timestamp":1772989014938,"version":"3.50.1"},"reference-count":57,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T00:00:00Z","timestamp":1752192000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T00:00:00Z","timestamp":1752192000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Long\u2010acting gonadotropin\u2010releasing hormone (GnRH) agonists are commonly used for fertility control in male dogs. Their effect on testicular histomorphology has not been clarified.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To elucidate the mechanisms underlying androgen withdrawal in response to long\u2010acting GnRH agonist deslorelin 4.7\u00a0mg treatment on testicular histomorphology and key extracellular matrix (ECM) and cytoskeletal components.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and methods<\/jats:title>\n                    <jats:p>\n                      Testes of deslorelin\u2010treated dogs (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a05) were evaluated at maximum downregulation of germinative and steroidogenic function, and compared with untreated (control) adult (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a07) and prepubertal immature (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a08) dogs. Morphological analysis was performed with Crossman's triple staining and periodic\u2010acid\u2010Schiff (PAS). Localization of smooth muscle \u03b1\u2010actin (\u03b1SMA\/ACTA2), desmin (DES), elastin (ELN), collagen type I (COL1A1), collagen type IV (COL4A1) was detected by immunohistochemistry.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Deslorelin treatment resulted in complete absence of germ cells except for spermatogonia. Crossman's triple staining identified thickening of peritubular connective tissue characterized by small infoldings toward the lumen of atrophied seminiferous tubules, and the expansion of interstitial area. PAS staining revealed a thickened basement membrane and phagosome\u2010like particles within the seminiferous tubules. In control dogs, \u03b1SMA\/ACTA2, DES, ELN, COL1A1, and COL4A1 were localized to blood vessels and the peritubular wall of seminiferous tubules. ELN, COL1A1, and COL4A1 were also found in stromal compartments. A similar localization pattern with signals within a thickened peritubular area was found in deslorelin\u2010treated dogs. Prepubertal dogs had similar distribution but weaker signal intensity in the peritubular wall of seminiferous tubules. Positive area percentages of \u03b1SMA\/ACTA2, DES, ELN, and COL4A1 were the highest in deslorelin\u2010treated dogs, while COL1A1 was the lowest in control, intermediate in deslorelin\u2010treated, and highest in prepubertal dogs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Deslorelin treatment caused infertility with accumulation of collagen (COL4A1, COL1A1) and deposition of \u03b1SMA\/ACTA2, DES, and ELN within the thickened lamina propria surrounding seminiferous tubules. Peritubular myocytes maintained their smooth muscle phenotype despite androgen withdrawal. The immature testis had lower abundance of most cytoskeletal and ECM proteins.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/andr.70086","type":"journal-article","created":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T11:04:34Z","timestamp":1752231874000},"page":"718-735","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Modified cytoskeletal and extracellular matrix composition in the canine testis after long\u2010acting gonadotropin\u2010releasing hormone agonist deslorelin treatment"],"prefix":"10.1111","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7490-5836","authenticated-orcid":false,"given":"Aykut","family":"Gram","sequence":"first","affiliation":[{"name":"Department of Histology and Embryology Faculty of Veterinary Medicine Erciyes University  Kayseri Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Murat","family":"Abay","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Faculty of Veterinary Medicine Erciyes University  Kayseri Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Duygu Yaman","family":"Gram","sequence":"additional","affiliation":[{"name":"Department of Physiology Faculty of Veterinary Medicine Erciyes University  Kayseri Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Narin","family":"Liman","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology Faculty of Veterinary Medicine Erciyes University  Kayseri Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Food Animal Medicine Clinic University of Veterinary Medicine Budapest  Budapest Hungary"},{"name":"ATRC Aurigon Ltd.  Dunakeszi Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Orsolya","family":"Balogh","sequence":"additional","affiliation":[{"name":"Department of Small Animal Clinical Sciences Virginia\u2010Maryland College of Veterinary Medicine  Blacksburg USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,7,11]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2021.08.017"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439\u20100531.2010.01705.x"},{"key":"e_1_2_10_4_1","first-page":"151","article-title":"Medical control of reproduction in the male dog with deslorelin subcutaneous implants","volume":"13","author":"Fontbonne A","year":"2021","journal-title":"Clin Theriogenol"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1071\/rd03039"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439\u20100531.2008.01060.x"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD07088"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.02.037"},{"key":"e_1_2_10_9_1","first-page":"255","article-title":"Use of a GnRH analogue implant to produce reversible long\u2010term suppression of reproductive function in male and female domestic dogs","volume":"57","author":"Trigg TE","year":"2001","journal-title":"J Reprod Fertil Suppl"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102826.2011.02271.x"},{"key":"e_1_2_10_11_1","unstructured":"NavarroC SchoberP.Pharmacodynamics and pharmacokinetics of a sustained\u2010release implant of deslorelin in companion animals.7th International Symposium on Canine and Feline Reproduction.2012. pp.177\u2010178."},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12306"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12058"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP\u201013\u20100195"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani12182343"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1530\/acta.0.1160413"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439\u20100531.2008.01066.x"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939\u20104640.1989.tb00140.x"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.1.06781"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.103.023606"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2002\u2010220786"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2002\u2010221035"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M501049200"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.104.028225"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.100.6.1941"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1679\/aohc.59.1"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2015.04.003"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.10111"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010021\u201025544\u20100"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102605.1990.tb00970.x"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.1.00694"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9112498"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1993.tb00397.x"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001500208"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm9040961"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02889001"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441\u2010018\u20102968\u2010x"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP\u201016\u20100588"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2047\u20102927.2012.00030.x"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.09\u2010138347"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1751731117002129"},{"key":"e_1_2_10_42_1","first-page":"25","article-title":"Suppression of testicular activity by a GnRH agonist in hypophysectomized, gonadotropin\u2010treated mice","volume":"14","author":"Iwasaki K","year":"1989","journal-title":"Tokai J Exp Clin Med"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939\u20104640.1987.tb00970.x"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1090690105"},{"key":"e_1_2_10_45_1","volume-title":"Manual of Histological Techniques","author":"Bancroft J","year":"1984"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2089"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod21.4.823"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092410109"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001880310"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2022.03.149"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2024.01.004"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2021.09.011"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.13603"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2011.08.030"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024\u20103205(92)90621\u2010u"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00319048"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo\u2010125\u20104\u20101829"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.11.007"}],"container-title":["Andrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/andr.70086","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/andr.70086","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/andr.70086","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T11:45:43Z","timestamp":1772970343000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/andr.70086"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,11]]},"references-count":57,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1111\/andr.70086"],"URL":"https:\/\/doi.org\/10.1111\/andr.70086","archive":["Portico"],"relation":{},"ISSN":["2047-2919","2047-2927"],"issn-type":[{"value":"2047-2919","type":"print"},{"value":"2047-2927","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,11]]},"assertion":[{"value":"2025-02-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}