{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T03:05:13Z","timestamp":1792638313431,"version":"4.5.0"},"reference-count":48,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T00:00:00Z","timestamp":1754956800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T00:00:00Z","timestamp":1754956800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The widespread usage of millimeter waves (MMW) in different spheres of the public domain necessitates to have a better understanding of any possible health impacts caused by them. The latest example of this is the global implementation of 5G technology.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>The present investigation aimed to study the effects of 35.5\u00a0GHz frequency on reproductive parameters of male Wistar rats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The study employed a randomized controlled design, with animals being assigned to control, sham\u2010exposed, and exposed groups, with six animals in each group (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a06). The experimental group was exposed to 35.5\u00a0GHz frequency for 2\u00a0h per day for 60 days. At the end of the exposure period, various sperm parameters such as sperm count, morphology, viability, and sperm mitochondrial activity were evaluated. The evaluation of oxidative stress was done by lipid peroxidation assay in testis homogenate. Superoxide dismutase, total sulfhydryl level, and total antioxidant capacity were assessed in testis homogenate. The DNA damage in the testis was assessed through the comet assay.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The results showed a significant decrease in sperm viability and count, as well as morphometric changes in testis histopathology. Lipid peroxidation testis was significantly elevated in the exposed group, while superoxide dismutase (SOD), total sulfhydryl level, and total antioxidant capacity were significantly decreased in the exposed group. The comet assay revealed a significant increase in DNA damage in the exposed group, indicating genotoxic effects.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>The observed changes in sperm parameters, testicular morphology, and oxidative stress markers highlight the potential for testicular tissue damage via oxidative mechanisms. These results underscore the need for further research to elucidate the biological implications of chronic MMW exposure.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Chronic exposure to 35.5\u00a0GHz frequency may negatively impact male reproductive function and testicular tissue, primarily through mechanisms involving oxidative stress.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/andr.70107","type":"journal-article","created":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T05:58:18Z","timestamp":1755064698000},"page":"594-602","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Oxidative stress and testicular damage induced by chronic exposure to 35.5\u00a0GHz millimeter wave radiation in male Wistar 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