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The axoneme consists of a conserved \u201c9+2\u201d microtubule arrangement and is supported by microtubule inner proteins. However, the functional significance of many microtubule inner proteins remains unclear. Cilia\u2010 and flagella\u2010associated protein 77 (CFAP77) has been identified as a microtubule inner protein in various species, but its role in mammalian sperm function has not been fully elucidated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      This study aims to investigate the function of CFAP77 in sperm motility and male fertility using a\n                      <jats:italic>Cfap77<\/jats:italic>\n                      knockout mouse model.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Cfap77<\/jats:italic>\n                      knockout mice were generated using the CRISPR\u2010Cas9 system. Male fertility was assessed by mating tests, and sperm motility was analyzed using computer\u2010assisted sperm analysis. Immunoblotting and immunoprecipitation\u2010mass spectrometry were performed to determine CFAP77's localization and interaction with other microtubule inner proteins.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We found that CFAP77 localized to the sperm flagella in mice. Moreover,\n                      <jats:italic>Cfap77<\/jats:italic>\n                      knockout males exhibited significantly reduced fertility, with impaired sperm motility despite normal morphology. Immunoprecipitation\u2010mass spectrometry analysis revealed that CFAP77 interacts with TEKTL1, and CFAP77 loss leads to a reduced amount of TEKTL1 in spermatozoa.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion and Conclusion<\/jats:title>\n                    <jats:p>We demonstrate that CFAP77 is crucial for sperm motility and male fertility. The interaction between CFAP77 and other microtubule inner proteins suggests a role in stabilizing other microtubule inner proteins and regulating flagellar function. These results provide insights into the molecular mechanisms of sperm motility regulation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/andr.70152","type":"journal-article","created":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T11:05:53Z","timestamp":1764414353000},"page":"1240-1248","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Microtubule Inner Protein CFAP77 Contributes to Sperm Motility and Male Fertility in Mice"],"prefix":"10.1111","volume":"14","author":[{"given":"Haoting","family":"Wang","sequence":"first","affiliation":[{"name":"Research Institute for Microbial Diseases The University of Osaka  Suita Osaka Japan"},{"name":"Graduate School of Pharmaceutical Sciences The University of Osaka  Suita Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anh Hoang","family":"Pham","sequence":"additional","affiliation":[{"name":"Research Institute for Microbial 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