{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T13:08:25Z","timestamp":1789996105093,"version":"4.0.1"},"reference-count":55,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T00:00:00Z","timestamp":1766534400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T00:00:00Z","timestamp":1766534400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100009101","name":"Education Department of Henan Province","doi-asserted-by":"publisher","award":["22A310017"],"award-info":[{"award-number":["22A310017"]}],"id":[{"id":"10.13039\/501100009101","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Asthenozoospermia is a significant contributing factor to male infertility. Accumulating evidence indicates that impaired sperm motility is closely linked to dysregulated microRNA expression during spermatogenesis. Seminal plasma exosomes are enriched with diverse microRNAs, which play pivotal roles in modulating sperm motility, the acrosome reaction, capacitation, and fertilization.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To investigate the association between exosomal microRNA profiles and sperm motility by comparing microRNA expression patterns in seminal plasma exosomes from asthenozoospermic patients and healthy controls, thereby elucidating the molecular mechanisms underlying exosomal microRNA\u2010mediated regulation of sperm motility.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>Seminal plasma exosomes were isolated from 21 asthenozoospermic patients and 21 age\u2010matched healthy donors via ultracentrifugation, followed by characterization via transmission electron microscopy, nanoparticle tracking analysis, and Western blotting. Exosomal microRNA profiles were analyzed via unique molecular identifier\u2010based small RNA sequencing to identify motility\u2010associated microRNAs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Comparative analysis revealed 13 significantly upregulated microRNAs in the exosomes of asthenozoospermic patients, among which hsa\u2010miR\u20107\u20105p exhibited the greatest abundance. Functional enrichment analysis revealed that the target genes of hsa\u2010miR\u20107\u20105p were associated primarily with cellular metabolism, macromolecule metabolic processes, and nitrogen compound metabolism, as well as several crucial signaling pathways, including mTOR signaling, endocrine resistance, and gonadotropin\u2010releasing hormone secretion. A dual\u2010luciferase reporter assay verified that hsa\u2010miR\u20107\u20105p directly targets rapidly accelerated fibrosarcoma 1, a pivotal component of the gonadotropin\u2010releasing hormone signaling pathway.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion and Conclusion<\/jats:title>\n                    <jats:p>hsa\u2010miR\u20107\u20105p may regulate sperm motility through the rapidly accelerated fibrosarcoma 1 \u2010mediated gonadotropin\u2010releasing hormone secretion and MAPK signaling networks. Our findings highlight the potential role of exosomal microRNAs in male infertility. Future prospective studies are warranted to elucidate the molecular mechanisms underlying the regulation of sperm motility.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/andr.70159","type":"journal-article","created":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T11:46:23Z","timestamp":1766576783000},"page":"2166-2179","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Seminal Plasma Exosomal miRNA Profiling Reveals hsa\u2010miR\u20107\u20105p as a Key Regulator of Sperm Motility in Asthenozoospermia"],"prefix":"10.1111","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6249-5374","authenticated-orcid":false,"given":"Zhi\u2010Jian","family":"Zhu","sequence":"first","affiliation":[{"name":"Affiliated Central Hospital Huanghuai University  Zhumadian China"},{"name":"College of Biological and Food Engineering Huanghuai University  Zhumadian China"},{"name":"Topfond Pharmaceutical Co., Ltd  Zhumadian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng\u2010Li","family":"Cui","sequence":"additional","affiliation":[{"name":"College of Biological and Food Engineering Huanghuai University  Zhumadian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen\u2010Jing","family":"Yuan","sequence":"additional","affiliation":[{"name":"College of Biological and Food Engineering Huanghuai University  Zhumadian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia\u2010Ning","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Biological and Food Engineering Huanghuai University  Zhumadian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuo","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Biological and Food Engineering Huanghuai University  Zhumadian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi\u2010Qiao","family":"Yao","sequence":"additional","affiliation":[{"name":"College of Biological and Food Engineering Huanghuai University  Zhumadian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruo\u2010Qing","family":"Li","sequence":"additional","affiliation":[{"name":"College of Biological and Food Engineering Huanghuai University  Zhumadian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-8310-5741","authenticated-orcid":false,"given":"Jin\u2010Feng","family":"Li","sequence":"additional","affiliation":[{"name":"Affiliated Central Hospital Huanghuai University  Zhumadian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7014-282X","authenticated-orcid":false,"given":"En\u2010Zhong","family":"Li","sequence":"additional","affiliation":[{"name":"College of Biological and Food Engineering Huanghuai University  Zhumadian China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,24]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10030666"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2024.101282"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.202033"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.4103\/aja202472"},{"issue":"1","key":"e_1_2_10_6_1","first-page":"602","article-title":"DNA Methylation Patterns in Patients With Asthenospermia and Oligoasthenospermia","volume":"25","author":"Zhang J.","year":"2024","journal-title":"BMC Genomics [Electronic Resource]"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.13828"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sjbs.2021.03.038"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12174"},{"key":"e_1_2_10_10_1","doi-asserted-by":"crossref","unstructured":"W. C.Chang S. H.Li andP. S.Tsai \u201cSeminal Vesicle\u2010Derived Exosomes for the Regulation of Sperm Activity \u201d (2024) https:\/\/doi.org\/10.1007\/102_2024_6.","DOI":"10.1007\/102_2024_6"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.70000"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-018-0897-7"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2019.06.008"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2019.01.030"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12958-018-0358-6"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/GCO.0000000000000621"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.RA118.001248"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2023.11.028"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2023.08.031"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.5534\/wjmh.250115"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dey072"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2016.06.030"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2020.11.020"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12714"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.13461"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19041233"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12801"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2011.169714"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1159\/000288242"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1413186112"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/frph.2024.1330161"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2021.758206"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dez114"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms251910569"},{"issue":"5","key":"e_1_2_10_35_1","first-page":"460","article-title":"Relationship of Small Non\u2010coding RNA in the Seminal Plasma With Male Infertility: An Update]","volume":"25","author":"Bian Y. Y.","year":"2019","journal-title":"Zhonghua Nan Ke Xue = National Journal of Andrology"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24043194"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2023.06.020"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21114089"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-025-92880-2"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2012.967"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2012.11.054"},{"issue":"8","key":"e_1_2_10_42_1","first-page":"686","article-title":"The miR\u2010184 Level in the Seminal Plasma Exosome of Male Infertility Patients and Its Clinical Significance]","volume":"26","author":"Zhu Y. Y.","year":"2020","journal-title":"Zhonghua Nan Ke Xue = National Journal of Andrology"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12913"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2024.1473825"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani15010040"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.2174\/1566524020666201203164910"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.13811"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.115.138016"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1177\/2050313X19834154"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmb2.12393"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2265.1998.00517.x"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.3389\/frph.2024.1330161"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12958-021-00723-2"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.180091"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2012.07.005"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.csbj.2020.05.001"}],"container-title":["Andrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/andr.70159","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/andr.70159","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/andr.70159","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T12:31:21Z","timestamp":1789993881000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/andr.70159"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,24]]},"references-count":55,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/andr.70159"],"URL":"https:\/\/doi.org\/10.1111\/andr.70159","archive":["Portico"],"relation":{},"ISSN":["2047-2919","2047-2927"],"issn-type":[{"value":"2047-2919","type":"print"},{"value":"2047-2927","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,24]]},"assertion":[{"value":"2025-06-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}