{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T11:19:10Z","timestamp":1791458350180,"version":"4.3.4"},"reference-count":45,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T00:00:00Z","timestamp":1770595200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T00:00:00Z","timestamp":1770595200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004271","name":"Sapienza Universit\u00e0 di Roma","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004271","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Fertility preservation in patients with testicular cancer remains a clinical priority, yet the optimal timing for sperm cryopreservation\u2014before or after orchidectomy\u2014remains a matter of debate.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>The aim of this study was to determine the optimal timing for semen cryopreservation and the best\u2010quality sample for ART. We evaluated various markers, including semen analysis, hormonal profiles, ultrasound analysis and sperm DNA fragmentation (SDF) before and after orchidectomy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Material and Methods<\/jats:title>\n                    <jats:p>Comprehensive evaluations were conducted, including semen analysis, hormonal profiling, testicular ultrasound, and SDF assessment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Post\u2010orchidectomy samples exhibited a significant decline in total sperm count and progressive motility, as well as an increase in morphological abnormalities. However, a notable and significant reduction in SDF was observed after surgery, suggesting improved chromatin integrity once the tumour had been removed. Elevated preoperative follicle\u2010stimulating hormone (FSH) and higher body mass index (BMI) were identified as risk factors for post\u2010operative oligozoospermia. Hormonal assessment revealed increased levels of FSH and luteinising hormone (LH) post\u2010surgery, with a slight decrease in total testosterone. A significant relationship emerged between testicular volume and changes in SDF: patients with larger contralateral testicular volume experienced a more pronounced improvement in DNA fragmentation, while testicular echotextural heterogeneity was associated with a diminished benefit.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion and Conclusion<\/jats:title>\n                    <jats:p>Although not all participants underwent full diagnostic evaluation, limiting certain analyses, the findings nonetheless underscore the importance of a detailed andrological assessment at the time of diagnosis. This should include semen analysis, hormonal evaluation and ultrasound examination of the contralateral testis to inform personalised fertility preservation strategies. Despite the deterioration in conventional semen parameters, the post\u2010orchidectomy sample demonstrated better DNA integrity and may thus represent the more suitable sample for use in assisted reproductive technologies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/andr.70190","type":"journal-article","created":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T07:30:54Z","timestamp":1770708654000},"page":"1272-1281","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Semen Cryopreservation in Testicular Cancer: Before or After Orchidectomy?"],"prefix":"10.1111","volume":"14","author":[{"given":"Alessandra","family":"Buonacquisto","sequence":"first","affiliation":[{"name":"Laboratory of Seminology \u201cLoredana Gandini\u201d Sperm Bank Department of Experimental Medicine \u201cSapienza\u201d University of Rome  Rome Italy"},{"name":"Department of Medicine and Surgery University of Enna \u201cKore\u201d  Enna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1147-6114","authenticated-orcid":false,"given":"Carlotta","family":"Pozza","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Andrology Department of Experimental Medicine Sapienza University  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaia","family":"Cicolani","sequence":"additional","affiliation":[{"name":"Laboratory of Seminology \u201cLoredana Gandini\u201d Sperm Bank Department of Experimental Medicine \u201cSapienza\u201d University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5747-4774","authenticated-orcid":false,"given":"Anna Chiara","family":"Conflitti","sequence":"additional","affiliation":[{"name":"Laboratory of Seminology \u201cLoredana Gandini\u201d Sperm Bank Department of Experimental Medicine \u201cSapienza\u201d University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luisa","family":"Caponecchia","sequence":"additional","affiliation":[{"name":"Laboratory of Seminology \u201cLoredana Gandini\u201d Sperm Bank Department of Experimental Medicine \u201cSapienza\u201d University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serena","family":"Bianchini","sequence":"additional","affiliation":[{"name":"Laboratory of Seminology \u201cLoredana Gandini\u201d Sperm Bank Department of Experimental Medicine \u201cSapienza\u201d University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrico Delli","family":"Paoli","sequence":"additional","affiliation":[{"name":"Laboratory of Seminology \u201cLoredana Gandini\u201d Sperm Bank Department of Experimental Medicine \u201cSapienza\u201d University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Tenuta","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Andrology Department of Experimental Medicine Sapienza University  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniele","family":"Gianfrilli","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Andrology Department of Experimental Medicine Sapienza University  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea M.","family":"Isidori","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Andrology Department of Experimental Medicine Sapienza University  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6943-2966","authenticated-orcid":false,"given":"Francesco","family":"Lombardo","sequence":"additional","affiliation":[{"name":"Laboratory of 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