{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T04:52:05Z","timestamp":1791435125592,"version":"4.3.3"},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T00:00:00Z","timestamp":1775779200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T00:00:00Z","timestamp":1775779200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Varicocoele is a major cause of male infertility, yet its underlying molecular mechanisms and determinants of surgical efficacy remain unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study aimed to identify key proteins involved in varicocoele\u2010related infertility and to investigate the expression and functional role of ubiquitin\u2010specific peptidase 2 in spermatozoa and spermatogenic cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Ubiquitin\u2010specific peptidase 2 expression in spermatozoa from varicocoele patients was analyzed before and after varicocelectomy by Western blotting. In vitro, ubiquitin\u2010specific peptidase 2 was overexpressed or silenced in mouse GC\u20102 spermatocyte cells to assess mitochondrial autophagy (PINK1\/Parkin pathway), autophagic flux, apoptosis, and reactive oxygen species. In vivo, a left\u2010sided varicocoele rat model was established, and ubiquitin\u2010specific peptidase 2 activity was inhibited by intratesticular injection of ML364. Testicular histopathology and sperm motility were evaluated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Ubiquitin\u2010specific peptidase 2 protein expression was significantly reduced in spermatozoa after varicocelectomy. Ubiquitin\u2010specific peptidase 2 overexpression enhanced PINK1\/Parkin\u2010mediated mitochondrial autophagy and protected spermatogenic cells from apoptosis, whereas ubiquitin\u2010specific peptidase 2 knockdown impaired mitochondrial autophagy and increased cell apoptosis. In varicocoele rats, ubiquitin\u2010specific peptidase 2 inhibition aggravated seminiferous tubule damage, reduced spermatogenic cell density, and impaired sperm motility. In human spermatozoa, ML364 treatment significantly decreased progressive motility without affecting sperm concentration.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Ubiquitin\u2010specific peptidase 2 is essential for maintaining mitochondrial quality control and spermatogenic cell survival in varicocoele by regulating PINK1\/Parkin\u2010mediated autophagy and oxidative stress, highlighting its potential as a biomarker and therapeutic target for varicocoele\u2010related male infertility.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/andr.70230","type":"journal-article","created":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T09:15:09Z","timestamp":1775812509000},"page":"1656-1668","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["USP2 Attenuates Spermatogenic Cell Apoptosis Via PINK1\/Parkin\u2010Dependent Mitophagy in Varicocoele: A Functional Study"],"prefix":"10.1111","volume":"14","author":[{"given":"Rui\u2010Jie","family":"Yao","sequence":"first","affiliation":[{"name":"Department of Andrology and Sexual Medicine, Urology Research Institute The First Affiliated Hospital of Fujian Medical University  Fuzhou China"},{"name":"Department of Andrology and Sexual Medicine, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital Fujian Medical University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Andrology and Sexual Medicine, Urology Research Institute The First Affiliated Hospital of Fujian Medical University  Fuzhou China"},{"name":"Department of Andrology and Sexual Medicine, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital Fujian Medical University  Fuzhou 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