{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T04:52:02Z","timestamp":1791435122882,"version":"4.3.3"},"reference-count":44,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T00:00:00Z","timestamp":1776297600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T00:00:00Z","timestamp":1776297600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82301821"],"award-info":[{"award-number":["82301821"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82271648"],"award-info":[{"award-number":["82271648"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Erectile dysfunction (ED) affects more than half of male patients with diabetes. Diabetes mellitus\u2010related ED (DMED) remains challenging to manage due to hyperglycemia\u2010induced apoptosis and fibrosis of corpus cavernosum smooth muscle cells (SMCs). Exosomes secreted by normal SMCs (NSMC\u2010EXOs) have emerged as promising therapeutic vectors. This study investigated the therapeutic role and molecular mechanism of NSMC\u2010EXO\u2010carried microRNA\u201030a\u20105p (miR\u201030a\u20105p) in DMED.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Erectile function in DMED rats treated with NSMC\u2010EXOs was assessed via electrophysiological testing. Exosomal miRNA profiles were characterized by sequencing, and potential target genes were identified through bioinformatic prediction. The interaction between miR\u201030a\u20105p and Calm1 was experimentally validated. Expression levels of miR\u201030a\u20105p, Calm1, and markers of apoptosis and fibrosis were examined in vivo and in vitro. MiR\u201030a\u20105p mimics and inhibitors were employed to confirm the regulatory mechanism.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Administration of NSMC\u2010EXOs significantly restored erectile function in DMED rats. Sequencing revealed a marked enrichment of miR\u201030a\u20105p in NSMC\u2010EXOs compared with exosomes from high\u2010glucose\u2010treated SMCs. Calm1 was identified as a direct target of miR\u201030a\u20105p, with involvement in apoptotic and fibrotic signaling pathways. Both in vivo and in vitro studies demonstrated that NSMC\u2010EXO\u2010derived miR\u201030a\u20105p was efficiently internalized by SMCs, suppressed Calm1 expression, and inhibited activation of the Calm1\u2010AMPK\u2010JNK and Calm1\u2010CaMK2\u2010TGF\u03b21 cascades. Consequently, apoptosis and fibrosis of corpus cavernosum tissue under hyperglycemic conditions were alleviated. Importantly, these protective effects were abolished following miR\u201030a\u20105p inhibition, underscoring its pivotal role.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>NSMC\u2010EXO\u2010derived miR\u201030a\u20105p protects against DMED by directly targeting Calm1 and attenuating pro\u2010apoptotic and pro\u2010fibrotic signaling. These findings provide mechanistic insight supporting the development of exosome\u2010based therapeutic approaches for DMED.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/andr.70234","type":"journal-article","created":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T07:45:48Z","timestamp":1776325548000},"page":"1669-1683","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Smooth Muscle Cell\u2010Derived Exosomal miR\u201030a\u20105p as a Novel Therapeutic Strategy for Erectile Dysfunction in Diabetes Mellitus"],"prefix":"10.1111","volume":"14","author":[{"given":"Jingyu","family":"Song","sequence":"first","affiliation":[{"name":"Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Institute of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-8589-6130","authenticated-orcid":false,"given":"Peng","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Institute of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0392-0596","authenticated-orcid":false,"given":"Jiaxin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Institute of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6947-427X","authenticated-orcid":false,"given":"Wenchao","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Institute of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Institute of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3055-8721","authenticated-orcid":false,"given":"Tao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Institute of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8909-3316","authenticated-orcid":false,"given":"Jihong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Institute of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7767-7309","authenticated-orcid":false,"given":"Yajun","family":"Ruan","sequence":"additional","affiliation":[{"name":"Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Institute of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,16]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-022-03147-w"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diabres.2021.109119"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.13291"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.01.043"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aau6977"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/sxmrev\/qeae022"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.15946"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2018.00402"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2022.948959"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2023.06.001"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13008-023-00099-2"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms242216197"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12929-023-00964-w"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2023.115449"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12483"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2012.12.001"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2020.01257"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/jsxmed\/qdae016"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.26271"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1521230113"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.824188"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-016-0366-z"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gpb.2015.02.001"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/1342190"},{"key":"e_1_2_9_26_1","first-page":"236","article-title":"MicroRNA\u201130a\u20115p Promotes Proliferation and Inhibits Apoptosis of Human Pulmonary Artery Endothelial Cells Under Hypoxia by Targeting YKL\u201140","volume":"20","author":"Tan H.","year":"2019","journal-title":"Molecular Medicine Reports"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsxm.2019.04.004"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2011.06.024"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.14908"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2010.6"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.13822"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2024.1436013"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/jsxmed\/qdae067"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/6834236"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201902086R"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.14212"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2021.112394"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.109689"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/jsxmed\/qdae129"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41585-018-0109-7"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10741-015-9498-3"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201902692R"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsxm.2020.04.390"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1177\/1753425920971347"},{"issue":"4","key":"e_1_2_9_45_1","first-page":"1539","article-title":"miR\u201130a\u20115p Inhibits Hypoxia\/Reoxygenation\u2011Induced Oxidative Stress and Apoptosis in HK\u20112 Renal Tubular Epithelial Cells by Targeting Glutamate Dehydrogenase 1 (GLUD1)","volume":"44","author":"He Y.","year":"2020","journal-title":"Oncology Reports"}],"container-title":["Andrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/andr.70234","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/andr.70234","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/andr.70234","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T04:47:48Z","timestamp":1791434868000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/andr.70234"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,16]]},"references-count":44,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1111\/andr.70234"],"URL":"https:\/\/doi.org\/10.1111\/andr.70234","archive":["Portico"],"relation":{},"ISSN":["2047-2919","2047-2927"],"issn-type":[{"value":"2047-2919","type":"print"},{"value":"2047-2927","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,16]]},"assertion":[{"value":"2025-09-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}