{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T07:11:38Z","timestamp":1783926698830,"version":"3.55.0"},"reference-count":18,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T00:00:00Z","timestamp":1776384000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T00:00:00Z","timestamp":1776384000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Reference intervals for calculated free testosterone (cFT) in healthy, nonobese men have been released, but have not been validated in men with erectile dysfunction (ED).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To assess the clinical impact of new cFT reference intervals in men with new\u2010onset ED.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Data from 410 healthy, nonobese men were analyzed (2015\u22122023). cFT was assessed using Vermeulen's formula and compared to Jasuja et\u00a0al.\u2019s (2022) reference values, using the 2.5th percentile as a pathological threshold. At baseline, all patients completed the International Index of Erectile Function (IIEF) and Beck Depression Inventory (BDI). Descriptive statistics and linear regression analyses were applied.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Median (IQR) age and total testosterone at presentation were 48 (38\u201359) years and 4.7 (3.3\u20136.1) ng\/mL. Median IIEF\u2010EF score was 18 (8\u201323), with 134 patients (32.8%) reporting severe ED. Percentiles of cFT in ED men were: 2.5th = 10, 10th = 50, 50th = 90, 90th = 150, and 97.5th = 227.5 pg\/mL, compared to 66, 91, 141, 240, and 309 pg\/mL in healthy controls. Seventy\u2010four had cFT between 10 and 66 pg\/mL, normal for ED distribution but pathological by healthy reference. This group was older (\n                      <jats:italic>p<\/jats:italic>\n                      &lt;0.01), had lower IIEF\u2010EF (\n                      <jats:italic>p<\/jats:italic>\n                      &lt;0.001), and higher BDI (\n                      <jats:italic>p<\/jats:italic>\n                      &lt;0.01) versus those with cFT &gt;66 pg\/mL, though other parameters were similar. Severe ED rates were 72.7%, 44.6%, and 28.7% in men with cFT &lt;10, 10\u201366, and &gt;66 pg\/mL (\n                      <jats:italic>p<\/jats:italic>\n                      &lt;0.01). At multivariable regression, cFT &gt;66 pg\/mL was linked to higher IIEF\u2010EF (\n                      <jats:italic>p<\/jats:italic>\n                      = 0.02), while cFT &gt;66 pg\/mL (\n                      <jats:italic>p<\/jats:italic>\n                      = 0.03) and younger age (\n                      <jats:italic>p<\/jats:italic>\n                      = 0.01) were associated with lower BDI scores.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Reference thresholds for cFT derived from healthy men identify, among men with ED, a subgroup showing a less favorable erectile and psychometric profile. These findings suggest that healthy\u2010derived cFT reference values may provide clinically useful information in the assessment of men presenting with ED.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/andr.70235","type":"journal-article","created":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T04:10:47Z","timestamp":1776485447000},"page":"1372-1379","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Are Reference Intervals for Calculated Free Testosterone in Healthy Men Reliable Also in Men With Erectile Dysfunction? Findings From a Cross\u2010Sectional Study"],"prefix":"10.1111","volume":"14","author":[{"given":"Federica","family":"Passarelli","sequence":"first","affiliation":[{"name":"Department of Urology Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0040-784X","authenticated-orcid":false,"given":"Luca","family":"Boeri","sequence":"additional","affiliation":[{"name":"Department of Urology Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0228-7039","authenticated-orcid":false,"given":"Edoardo","family":"Pozzi","sequence":"additional","affiliation":[{"name":"Division of Experimental Oncology\/Unit of Urology; URI; IRCCS Ospedale San Raffaele  Milan Italy"},{"name":"Vita\u2010Salute San Raffaele University  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3677-6412","authenticated-orcid":false,"given":"Fausto","family":"Negri","sequence":"additional","affiliation":[{"name":"Division of Experimental Oncology\/Unit of Urology; URI; IRCCS Ospedale San Raffaele  Milan Italy"},{"name":"Vita\u2010Salute San Raffaele University  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimiliano","family":"Raffo","sequence":"additional","affiliation":[{"name":"Division of Experimental Oncology\/Unit of Urology; URI; IRCCS Ospedale San Raffaele  Milan Italy"},{"name":"Vita\u2010Salute San Raffaele University  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9603-1589","authenticated-orcid":false,"given":"Christian","family":"Corsini","sequence":"additional","affiliation":[{"name":"Division of Experimental Oncology\/Unit of Urology; URI; IRCCS Ospedale San Raffaele  Milan Italy"},{"name":"Vita\u2010Salute San Raffaele University  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1298-8231","authenticated-orcid":false,"given":"Alessandro","family":"Bertini","sequence":"additional","affiliation":[{"name":"Division of Experimental Oncology\/Unit of Urology; URI; IRCCS Ospedale San Raffaele  Milan Italy"},{"name":"Vita\u2010Salute San Raffaele University  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessia","family":"d'Arma","sequence":"additional","affiliation":[{"name":"Division of Experimental Oncology\/Unit of Urology; URI; IRCCS Ospedale San Raffaele  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuele","family":"Montanari","sequence":"additional","affiliation":[{"name":"Department of Urology Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico  Milan Italy"},{"name":"Dipartimento Di Scienze Cliniche e Di Comunit\u00e0 Dipartimento Di Eccellenza 2023\u20132027  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Montorsi","sequence":"additional","affiliation":[{"name":"Division of Experimental Oncology\/Unit of Urology; URI; IRCCS Ospedale San Raffaele  Milan Italy"},{"name":"Vita\u2010Salute San Raffaele University  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0595-7165","authenticated-orcid":false,"given":"Andrea","family":"Salonia","sequence":"additional","affiliation":[{"name":"Division of Experimental Oncology\/Unit of Urology; URI; IRCCS Ospedale San Raffaele  Milan Italy"},{"name":"Vita\u2010Salute San Raffaele University  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,17]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572\u2010019\u20100087\u2010y"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/sxmrev\/qeaf036"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41443\u2010025\u201001029\u20102"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2025.12.015"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2025.04.010"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2018\u201000229"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1530\/EC\u201020\u20100183"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsxm.2017.10.070"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/bju.14899"},{"key":"e_1_2_10_11_1","unstructured":"G.Tsampoukas S.Karna A.Morgado andS.Minhas \u201cVariations in Diagnostic Criteria for Male Hypogonadism: Is There a Need for Standardizing Specialist Society Guidelines? 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