{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T07:11:41Z","timestamp":1783926701056,"version":"3.55.0"},"reference-count":24,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T00:00:00Z","timestamp":1776729600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T00:00:00Z","timestamp":1776729600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Semen analysis remains the first\u2010line diagnostic test for male infertility, although it is affected by operator subjectivity. Computer\u2010assisted sperm analysis (CASA) is proposed to improve objectivity; however, its accuracy and clinical relevance remain uncertain.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>We evaluated 424 samples from men attending routine semen analysis and 179 samples from male partners of ICSI couples to compare manual assessment with a CASA system (Ceros II), and to explore whether CASA\u2010derived kinematic parameters, in particular hyperactivated motility (HA), predict ICSI outcomes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>Manual (according to WHO manual) and CASA assessments were compared using correlation analysis, Passing\u2013Bablok regression, and Bland\u2013Altman plots; kinematic parameters were evaluated in relation to ICSI outcomes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Significant correlations were found between CASA and manual assessment for rapid progressive motility and for sperm count for samples with less than 50 \u00d7 10\n                      <jats:sup>6<\/jats:sup>\n                      \/mL spermatozoa, whereas agreement was lost above this value, unless dilution was performed. Among CASA kinematic parameters, HA measured in whole semen was significantly higher in cycles achieving clinical pregnancy and resulted an independent pregnancy predictor also after adjustment for female age and female factor, showing good discriminative performance.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>These findings suggest that CASA provides objective, reproducible assessment of sperm motility, and that hyperactivated motility may serve as a clinically relevant marker to improve prediction of ICSI outcomes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>CASA may provide useful support for routine semen analysis and identify clinically meaningful kinematic parameters.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Trial Registration<\/jats:title>\n                    <jats:p>Not applicable<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/andr.70242","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T07:36:22Z","timestamp":1776756982000},"page":"1401-1412","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Computer\u2010Assisted Sperm Analysis (CASA) Versus Manual Evaluation and the Clinical Significance of Sperm Hyperactivated Motility"],"prefix":"10.1111","volume":"14","author":[{"given":"Maria Emanuela","family":"Ragosta","sequence":"first","affiliation":[{"name":"Department of Experimental and Clinical Biomedical Sciences \u201cMario Serio\u201d University of Florence  Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulia","family":"Traini","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Biomedical Sciences \u201cMario Serio\u201d University of Florence  Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Benini","sequence":"additional","affiliation":[{"name":"IVIRMA Global Research Alliance, Demetra  Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margherita","family":"Montereggi","sequence":"additional","affiliation":[{"name":"IVIRMA Global Research Alliance, Demetra  Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federica","family":"Bini","sequence":"additional","affiliation":[{"name":"IVIRMA Global Research Alliance, Demetra  Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lara","family":"Tamburrino","sequence":"additional","affiliation":[{"name":"Andrology and Gender Endocrinology Unit, Center for Prevention, Diagnosis and Treatment of Infertility Careggi University Hospital  Florence 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