{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T05:49:12Z","timestamp":1791438552646,"version":"4.3.3"},"reference-count":47,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T00:00:00Z","timestamp":1780012800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T00:00:00Z","timestamp":1780012800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["305977\/2024\u20105"],"award-info":[{"award-number":["305977\/2024\u20105"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Sperm acquire fertility ability during epididymal maturation mainly in the epididymal caput and corpus, and once matured, are stored in the epididymal cauda. During storage, interactions with cauda epididymosomes (epEVs) may influence sperm fertility potential; however, the role of such interactions on sperm fertility remains elusive.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To investigate the effect of epEVs on sperm fertility potential using the bovine model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>A pool of epEVs from cauda epididymal fluid of five bovine males was characterized regarding size, concentration, morphology, and specific markers. To determine the sperm\u2013epEVs interaction protocol, green\u2010labeled epEVs (PKH67) were incubated with post\u2010thawed cauda epididymal sperm from three bovine males at ratios of 500, 1000, or 2000 epEVs\/sperm, and for incubation periods of 1.5, 3, or 6 h. After that, Hoechst\u2010stained sperm were analyzed by flow cytometer. Green fluorescence percentage and intensity of 5000 positive Hoechst events were considered as sperm\u2013epEVs interacting. Controls were performed by incubating sperm with PKH67 in PBS. A total of 49 microRNAs (out of 380 investigated) were found detected in the epEVs and the top five were investigated in sperm by quantitative polymerase chain reaction (qPCR) following sperm\u2013epEVs interaction. Subsequently, sperm that interacted with epEVs were used to produce embryos by in vitro fertilization.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      epEVs displayed 114.20 \u00b1 3.60\u00a0nm, 3.48\u00a0\u00d7\u00a010\n                      <jats:sup>9<\/jats:sup>\n                      \u00b1 1.84\u00a0\u00d7\u00a010\n                      <jats:sup>8<\/jats:sup>\n                      particles\/mL, a cup\u2010shaped morphology, and positivity for ALIX, CD\u201081, and CD\u201063 markers. Following determination of the protocol (1000 epEVs\/sperm for 3\u00a0h), two microRNAs (miR\u2010935 and \u2010421) previously detected in epEVs, were found in sperm. Finally, sperm incubated with epEVs resulted in higher (p = 0.04) blastocyst rates (epEVs: 38.9% \u00b1 7.3%; 58\/149; control: 26.6% \u00b1 5.6%; 40\/145).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion and Conclusion<\/jats:title>\n                    <jats:p>Incubation of sperm with 1000 epEVs\/sperm for 3 h promoted sperm\u2013epEVs in vitro crosstalk that enhanced blastocyst rates. These findings suggest that epEVs modulate paternal contribution to development and provide valuable insights to promote a fast and dynamic control of male fertility.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/andr.70267","type":"journal-article","created":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T06:44:33Z","timestamp":1780123473000},"page":"1757-1770","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["In Vitro Sperm\u2013Epididymosomes Interaction Immediately Before Fertilization Changes Sperm Fertility Potential"],"prefix":"10.1111","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9480-6608","authenticated-orcid":false,"given":"Ma\u00edra Bianchi Rodrigues","family":"Alves","sequence":"first","affiliation":[{"name":"Department of Pathology Theriogenology and One Healthy School of Agricultural and Veterinary Sciences S\u00e3o Paulo State University  Jaboticabal S\u00e3o 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Braido","family":"Rangel","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine School of Animal Science and Food Engineering University of S\u00e3o Paulo  Pirassununga S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fl\u00e1vio Vieira","family":"Meirelles","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine School of Animal Science and Food Engineering University of S\u00e3o Paulo  Pirassununga S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4796-6393","authenticated-orcid":false,"given":"Juliano Coelho","family":"da Silveira","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine School of Animal Science and Food Engineering University of S\u00e3o Paulo  Pirassununga S\u00e3o Paulo 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