{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T04:52:06Z","timestamp":1791435126221,"version":"4.3.3"},"reference-count":17,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T00:00:00Z","timestamp":1781136000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T00:00:00Z","timestamp":1781136000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014361","name":"Hubei University of Medicine","doi-asserted-by":"publisher","award":["2025QDJZR01"],"award-info":[{"award-number":["2025QDJZR01"]}],"id":[{"id":"10.13039\/501100014361","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Caseinolytic peptidase P (ClpP) plays a key role in maintaining cellular homeostasis for mitochondrial quality control. However, the specific function of ClpP during meiosis and its subcellular localization in spermatocytes remain poorly understood.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To investigate the function of ClpP in spermatocyte meiosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      ClpP expression was examined in mouse spermatocytes, and tamoxifen was utilized to achieve spatiotemporal\u2010specific deletion of\n                      <jats:italic>Clpp<\/jats:italic>\n                      mediated by\n                      <jats:italic>Ddx4<\/jats:italic>\n                      \u2010Cre\n                      <jats:sup>ERT2<\/jats:sup>\n                      in spermatocytes. We analyzed the meiotic progression of\n                      <jats:italic>Clpp<\/jats:italic>\n                      conditional KO (\n                      <jats:italic>Clpp<\/jats:italic>\n                      <jats:sup>cKO<\/jats:sup>\n                      ) using spermatocyte chromosome spreading, combined with immunofluorescence and transmission electron microscopy, to determine the morphology and number of spermatocyte mitochondria in\n                      <jats:italic>Clpp<\/jats:italic>\n                      <jats:sup>cKO<\/jats:sup>\n                      mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A progressive increase in ClpP expression levels was evident from the leptotene stage to the pachytene stage in mouse spermatocytes, and a decrease in ClpP expression was observed from the diplotene stage to the metaphase I (MI) stage. Compared with wild\u2010type male mice, adult\n                      <jats:italic>Clpp<\/jats:italic>\n                      <jats:sup>cKO<\/jats:sup>\n                      male mice had reduced testis size and no mature spermatozoa in their epididymides. A large proportion of pachytene and diplotene spermatocytes, as well as round or elongated spermatids, were eliminated from the seminiferous tubules of the\n                      <jats:italic>Clpp<\/jats:italic>\n                      <jats:sup>cKO<\/jats:sup>\n                      mice. The mitochondria of\n                      <jats:italic>Clpp<\/jats:italic>\n                      <jats:sup>cKO<\/jats:sup>\n                      spermatocytes appeared as \u201cgiant mitochondria.\u201d However,\n                      <jats:italic>Clpp<\/jats:italic>\n                      <jats:sup>cKO<\/jats:sup>\n                      spermatocytes exhibited normal meiotic synapsis and impaired recombination, with reduced RAD51 foci and abnormal MLH1 localization.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>ClpP is critical for spermatocyte survival and mitochondrial integrity during meiosis. As a consequence of its deficiency, meiotic progression and spermatogenesis are disrupted, highlighting its essential role in the meiosis of spermatocytes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/andr.70274","type":"journal-article","created":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T06:46:19Z","timestamp":1781246779000},"page":"1777-1788","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["ClpP Ensures Mitochondrial Integrity and Spermatocyte Meiotic Progression in Mice"],"prefix":"10.1111","volume":"14","author":[{"given":"Hai\u2010Wei","family":"Feng","sequence":"first","affiliation":[{"name":"Guangdong Key Laboratory of Urology Department of Urology The First Affiliated Hospital of Guangzhou Medical University  Guangzhou China"},{"name":"Department of Reproductive Health and Infertility South China Hospital Medical School Shenzhen University  Shenzhen China"},{"name":"Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging National\u2010Regional Key Technology Engineering Laboratory for Medical Ultrasound School of Biomedical Engineering Shenzhen University Medical School  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan\u2010Lin","family":"Gao","sequence":"additional","affiliation":[{"name":"Maternal\u2010Fetal Medicine Institute Department of Obstetrics and Gynecology Shenzhen Baoan Women's and Children's Hospital Jinan University  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin\u2010Jie","family":"Jiang","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory of Fertility Regulation Reproductive Medicine Center The University of Hong Kong\u2010Shenzhen Hospital  Shenzhen China"},{"name":"Department of Obstetrics and Gynecology Li Ka Shing Faculty of Medicine The University of Hong Kong  Hong Kong SAR 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