{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T06:27:48Z","timestamp":1786343268800,"version":"build-2736575974"},"reference-count":44,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T00:00:00Z","timestamp":1786233600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T00:00:00Z","timestamp":1786233600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Testicular germ cell tumors (TGCTs) are the most common malignancy in young adult men, and the development of reliable non\u2010invasive diagnostic tools remains a clinical priority. Seminal plasma is a complex yet accessible biofluid that reflects the function of the male reproductive system and may capture metabolic alterations associated with testicular pathology.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study aimed to characterize the seminal plasma metabolome of TGCT patients using a multi\u2010platform metabolomics strategy that covers polar metabolites and oxylipins.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>Seminal plasma from 37 TGCT patients and 11 non\u2010TGCT clinical controls was analyzed by capillary electrophoresis\u2010mass spectrometry (CE\u2010MS) and oxylipin profiling using liquid chromatography coupled to tandem mass spectrometry (LC\u2010MS\/MS). Immunohistochemical analysis of testicular tumor tissue was used to confirm enzymatic expression degree.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      CE\u2010MS revealed coordinated alterations in polar metabolites, particularly many involved in energy metabolism, suggesting a disruption of testicular metabolic homeostasis in TGCTs. Reduced levels of key energy\u2010related amino acids in plasma suggested increased metabolic demand in tumor cells, consistent with altered biosynthetic and redox pathways. Additional changes in hexosamine pathway metabolites indicated potential alterations in protein glycosylation and secretory activity. Oxylipin profiling using targeted LC\u2010MS\/MS identified several lipid mediators with significant differences between groups. Four compounds (9,10\u2010DiHOME, 6\u2010\n                      <jats:italic>trans<\/jats:italic>\n                      \u2010LTB\n                      <jats:sub>4<\/jats:sub>\n                      , TXB\n                      <jats:sub>2<\/jats:sub>\n                      , and 14,15\u2010DiHETrE) were elevated in TGCT samples and demonstrated discriminatory potential in univariate analyses. A composite oxylipin ratio of these compounds achieved excellent diagnostic performance, indicating that oxylipin\u2010based signatures may support TGCT detection. Immunohistochemical staining confirmed arachidonate 12\u2010lipoxygenase (ALOX12) expression in tumor tissue, confirming biological plausibility for the observed oxylipin alterations.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion and Conclusion<\/jats:title>\n                    <jats:p>Seminal plasma carries detectable metabolic signatures of TGCT, encompassing alterations in energy metabolism and inflammation\u2010related lipid mediators. These findings establish a foundation for developing non\u2010invasive metabolomic biomarkers for TGCT and warrant validation in larger and independent cohorts.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/andr.70347","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T05:29:48Z","timestamp":1786339788000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Metabolic and Oxylipin Signature of Testicular Germ Cell Tumors in Seminal Plasma"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-9205-4822","authenticated-orcid":false,"given":"Luz","family":"Alonso\u2010Dasques","sequence":"first","affiliation":[{"name":"Centro de Metabol\u00f3mica y Bioan\u00e1lisis (CEMBIO) Facultad de Farmacia Universidad San Pablo\u2010CEU CEU Universities Urbanizaci\u00f3n Montepr\u00edncipe  Boadilla del Monte Madrid 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