{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T16:24:07Z","timestamp":1787156647116,"version":"build-2736575974"},"reference-count":54,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000071","name":"National Institute of Child Health and Human Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000071","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>SUMO proteins are highly expressed in testicular cells, including spermatocytes and Sertoli, where they modify numerous proteins. Nonetheless, the role of SUMO proteins in spermatogenesis in vivo has remained unconfirmed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To confirm the requirement for sumoylation in the mouse in vivo during meiosis and in testicular Sertoli cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      To inactivate sumoylation, we targeted the UBA2 gene, a subunit of the only known SUMO\u2010activating enzyme.\n                      <jats:italic>Stra8<\/jats:italic>\n                      \u2010\n                      <jats:italic>Cre<\/jats:italic>\n                      ,\n                      <jats:italic>Amh<\/jats:italic>\n                      \u2010\n                      <jats:italic>Cre<\/jats:italic>\n                      , and\n                      <jats:italic>\n                        Uba2\n                        <jats:sup>fx\/fl<\/jats:sup>\n                      <\/jats:italic>\n                      mice were used to produce\n                      <jats:italic>Uba2<\/jats:italic>\n                      <jats:sup>fl\/fl<\/jats:sup>\n                      \u2010Cre mice with the conditional deletion of\n                      <jats:italic>Uba2<\/jats:italic>\n                      in either early meiotic or Sertoli cells.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Both mouse models have exhibited complete male infertility, a significant decrease in testicular weight, and spermatogenic arrest. Inactivation of sumoylation in spermatocytes led to meiotic arrest and misregulated expression of many genes involved in meiotic completion and spermatid differentiation. Inactivation of sumoylation in Sertoli cells leads to loss of germ cells, progressive testicular degeneration, and misregulated expression of genes that control Sertoli cell function, including those involved in germ cell adhesion and junctional complex formation. Transcription factor WT1 may be an important target of sumoylation in Sertoli cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>Sumoylation may provide a dual\u2010layer regulatory mechanism through direct binding to proteins required for meiosis and Sertoli cell functions, and indirect control of transcriptional events through regulation of transcription factors and chromatin modifiers. Future studies will address the mechanisms by which sumoylation controls transcription and other events in testicular cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>UBA2\u2010mediated sumoylation is required for both spermatocyte and Sertoli cell functions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/andr.70362","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T15:25:43Z","timestamp":1787153143000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["UBA2\u2010Mediated Sumoylation Is Required for Both Spermatocyte and Sertoli Cell Functions During Mouse Spermatogenesis"],"prefix":"10.1111","author":[{"given":"Sayanto Subrato","family":"Pal","sequence":"first","affiliation":[{"name":"Department of Biology Stern College Yeshiva University  New York New York USA"},{"name":"Biotechnology Management and Entrepreneurship program Katz School of Science and Health Yeshiva University  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Lucas","sequence":"additional","affiliation":[{"name":"Department of Biology Stern College Yeshiva University  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rupali","family":"Khane","sequence":"additional","affiliation":[{"name":"Department of Biology Stern College Yeshiva University  New York New York USA"},{"name":"Biotechnology Management and Entrepreneurship program Katz School of Science and Health Yeshiva University  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aliza","family":"Balsam","sequence":"additional","affiliation":[{"name":"Department of Biology Stern College Yeshiva University  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-5136-8219","authenticated-orcid":false,"given":"Yael","family":"Polotsky","sequence":"additional","affiliation":[{"name":"Department of Biology Stern College Yeshiva University  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Washington State University  Pullman Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6371-8931","authenticated-orcid":false,"given":"Hayden","family":"McSwiggin","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Washington State University  Pullman Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9569-9026","authenticated-orcid":false,"given":"Wei","family":"Yan","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Washington State University  Pullman Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0292-4800","authenticated-orcid":false,"given":"Margarita","family":"Vigodner","sequence":"additional","affiliation":[{"name":"Department of Biology Stern College Yeshiva University  New York New York USA"},{"name":"Department of Developmental and Molecular Biology Albert Einstein College of Medicine  Bronx New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,19]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00009.2002"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M402273200"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2004.09.021"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-005-0645-5"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.25.10.4272-4282.2005"},{"issue":"6","key":"e_1_2_10_7_1","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1038\/sj.embor.7400980","article-title":"SUMO Junction\u2010What's Your Function? 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