{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T15:17:08Z","timestamp":1787843828346,"version":"build-2784847793"},"reference-count":40,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:00:00Z","timestamp":1787788800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:00:00Z","timestamp":1787788800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82101693"],"award-info":[{"award-number":["82101693"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Hyperlipidemia\u2010related erectile dysfunction (HLED) responds poorly to first\u2010line treatments such as phosphodiesterase type 5 inhibitors (PDE5Is), emphasizing the need for alternative therapeutic strategies. Rutaecarpine (RUT), a bioactive alkaloid, exhibits significant anti\u2010inflammatory and anti\u2010fibrotic effects. However, its therapeutic potential and mechanisms in HLED remain unexplored.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To evaluate the therapeutic effects and underlying mechanisms of RUT on HLED in vivo and in vitro.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In vivo, 48 male Sprague\u2010Dawley rats were allocated into four groups: control, HLED, HLED+RUT\u2010LD (low dose), and HLED+RUT\u2010HD (high dose). Hyperlipidemia was induced through a high\u2010fat diet, and RUT was administered orally. Erectile function was assessed using cavernous manometry, followed by analysis of penile tissues. In vitro, NR8383s were treated with lipopolysaccharide to induce M1 polarization and co\u2010cultured with corpus cavernosum fibroblasts (CCFBs) to evaluate the impact of RUT on inflammation and fibrosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>RUT treatment partially improved erectile function in HLED rats. RUT reduced macrophage M1 polarization and fibrosis in penile tissues. In vitro, RUT inhibited LPS\u2010induced M1 macrophage polarization and reduced fibrosis\u2010related phenotype in co\u2010cultured CCFBs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>RUT could enhance erectile function in HLED rats by inhibiting macrophage M1 polarization and associated corpus cavernosum fibrosis. This study suggested that RUT could be a promising drug for treating HLED.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/andr.70370","type":"journal-article","created":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T14:18:29Z","timestamp":1787840309000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rutaecarpine Improves Hyperlipidemia\u2010Related Erectile Dysfunction in Rats by Inhibiting Macrophage M1 Polarization\u2010Induced Corpus Cavernosum Fibrosis"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-8929-1416","authenticated-orcid":false,"given":"Bocheng","family":"Tu","sequence":"first","affiliation":[{"name":"Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Institute of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiqing","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Institute of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Institute of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Institute of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0392-0596","authenticated-orcid":false,"given":"Jiaxin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Institute of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinqi","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Institute of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sen","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Institute of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3055-8721","authenticated-orcid":false,"given":"Tao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Institute of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8909-3316","authenticated-orcid":false,"given":"Jihong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Institute of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Institute of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,27]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"crossref","unstructured":"Impotence: NIH Consensus Development Panel on Impotence. JAMA270 no.1(1993):83\u201390 https:\/\/doi.org\/10.1001\/jama.1993.03510010089036.","DOI":"10.1001\/jama.1993.03510010089036"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacl.2012.05.002"},{"issue":"3","key":"e_1_2_11_4_1","first-page":"161","article-title":"Hyperlipidemia and Erectile Dysfunction","volume":"2","author":"Kim S. C.","year":"2000","journal-title":"Asian Journal of Andrology"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(85)92023\u20109"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/7286958"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsxm.2016.12.012"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI28898"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1743\u20106109.2008.01029.x"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00260.2018"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2023.1199799"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2024.117499"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules15031873"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2018.12.019"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201900903RRR"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2024.111790"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035\u2010023\u201003505\u20106"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23115889"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23010092"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2019.01.038"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964\u2010024\u201001828\u2010y"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1444964"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.803037"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.aaz0749"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2018.05.007"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsxm.2016.01.016"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijcp.12074"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010022\u201031950\u20109"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.37799"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967\u2010024\u201005381\u20107"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu15051177"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12345"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389450116666150420145757"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/iid3.1363"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2007.08.004"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/sxmrev\/qead055"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI28570"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2022.1029650"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.03697\u20102020"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11883\u2010017\u20100678\u20106"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.4103\/aja202324"}],"container-title":["Andrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/andr.70370","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/andr.70370","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/andr.70370","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T14:18:31Z","timestamp":1787840311000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/andr.70370"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,27]]},"references-count":40,"alternative-id":["10.1111\/andr.70370"],"URL":"https:\/\/doi.org\/10.1111\/andr.70370","archive":["Portico"],"relation":{},"ISSN":["2047-2919","2047-2927"],"issn-type":[{"value":"2047-2919","type":"print"},{"value":"2047-2927","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,27]]},"assertion":[{"value":"2024-12-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"andr.70370"}}