{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T07:58:39Z","timestamp":1790668719924,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Diet is known to play an essential role not only in sperm quality but also in offspring health, likely mediated through the sperm epigenome. Moreover, one of the main components of the Mediterranean diet (MedDiet) (i.e., nuts) have shown to impact sperm DNA methylation patterns.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study aims to investigate the association between adherence to the MedDiet and sperm DNA methylation patterns in healthy men.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Material\/Methods<\/jats:title>\n                    <jats:p>\n                      This cross\u2010sectional study was conducted on 60 healthy males of reproductive age (18\u201340 years) randomly selected, based on the MedDiet adherence level (high vs. low adherence), from 200 participants enrolled for the Lifestyle and Environmental Determinants of Seminogram and Other Male Fertility\u2010Related Parameters (Led\u2010Fertyl) study. Detailed lifestyle and dietary information and semen samples were collected. Sperm DNA was extracted and subjected to bisulfite conversion and then hybridized to Infinium MethylationEPIC v2.0 array according to established protocols. Sperm DNA methylation was analyzed using R statistical software, with CpG sites considered differentially methylated when showing an absolute mean methylation difference \u2265 0.10 between high and low adherence groups and an adjusted\n                      <jats:italic>p<\/jats:italic>\n                      \u2010value &lt; 0.05\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      There were no significant differences in the general and anthropometric characteristics between participants with high adherence and low adherence to MedDiet. Supervised differential methylation analyses found that there were 42 significantly differentially methylated CpGs, with 17 hypermethylated and 25 hypomethylated CpG sites in the participants with high adherence (\n                      <jats:italic>n<\/jats:italic>\n                      = 30) to MedDiet compared to the participants with low adherence (\n                      <jats:italic>n<\/jats:italic>\n                      = 30), after adjustment for age, smoking status, and BMI. The genes involved within differentially methylated sites underwent a gene ontology (GO) analysis, and we observed that 12 GO terms were significantly enriched (adjusted\n                      <jats:italic>p<\/jats:italic>\n                      \u2010value &lt; 0.05), mainly related to cyclic nucleotide signaling, ion channel activity, membrane trafficking\/endocytosis, cytoskeletal organization, and transcriptional regulation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>These novel and preliminary findings point to a possible relationship between diet quality and the sperm epigenome. Further studies should seek to confirm these observations in larger, more diverse groups including older men and individuals.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Overall, our findings suggest that adherence to the MedDiet, a dietary pattern rich in natural methyl donors and cofactors, may modulate sperm DNA methylation at specific genomic loci.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/andr.70398","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T07:47:17Z","timestamp":1790668037000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Differential Sperm DNA Methylation Profiles in Healthy Men With High Versus Low Adherence to the Mediterranean Diet in the Led\u2010Fertyl Study"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-3938-1652","authenticated-orcid":false,"given":"Rashmi","family":"Agarwal","sequence":"first","affiliation":[{"name":"Unitat de Nutrici\u00f3 Humana. Departament de Bioqu\u00edmica i Biotecnologia. Grup Alimentaci\u00f3, Nutrici\u00f3, Desenvolupament i Salut Mental (ANUT\u2010DSM) Universitat Rovira i Virgili  Reus Spain"},{"name":"Centro De Investigaci\u00f3n Biom\u00e9dica En Red de Fisiopatolog\u00eda de La Obesidad y Nutrici\u00f3n (CIBEROBN) Instituto De Salud Carlos III  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3527-5277","authenticated-orcid":false,"given":"Nancy","family":"Babio","sequence":"additional","affiliation":[{"name":"Unitat de Nutrici\u00f3 Humana. Departament de Bioqu\u00edmica i Biotecnologia. Grup Alimentaci\u00f3, Nutrici\u00f3, Desenvolupament i Salut Mental (ANUT\u2010DSM) Universitat Rovira i Virgili  Reus Spain"},{"name":"Centro De Investigaci\u00f3n Biom\u00e9dica En Red de Fisiopatolog\u00eda de La Obesidad y Nutrici\u00f3n (CIBEROBN) Instituto De Salud Carlos III  Madrid Spain"},{"name":"Institut de Recerca Biom\u00e8dica Catalunya Sud (IRBCatSud), former IISPV  Reus Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1153-9397","authenticated-orcid":false,"given":"Estefan\u00eda","family":"Davila\u2010Cordova","sequence":"additional","affiliation":[{"name":"Unitat de Nutrici\u00f3 Humana. Departament de Bioqu\u00edmica i Biotecnologia. Grup Alimentaci\u00f3, Nutrici\u00f3, Desenvolupament i Salut Mental (ANUT\u2010DSM) Universitat Rovira i Virgili  Reus Spain"},{"name":"Institut de Recerca Biom\u00e8dica Catalunya Sud (IRBCatSud), former IISPV  Reus Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Fern\u00e1ndez de la","family":"Puente","sequence":"additional","affiliation":[{"name":"Unitat de Nutrici\u00f3 Humana. Departament de Bioqu\u00edmica i Biotecnologia. Grup Alimentaci\u00f3, Nutrici\u00f3, Desenvolupament i Salut Mental (ANUT\u2010DSM) Universitat Rovira i Virgili  Reus Spain"},{"name":"Centro De Investigaci\u00f3n Biom\u00e9dica En Red de Fisiopatolog\u00eda de La Obesidad y Nutrici\u00f3n (CIBEROBN) Instituto De Salud Carlos III  Madrid Spain"},{"name":"Institut de Recerca Biom\u00e8dica Catalunya Sud (IRBCatSud), former IISPV  Reus Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Valle\u2010Hita","sequence":"additional","affiliation":[{"name":"Unitat de Nutrici\u00f3 Humana. Departament de Bioqu\u00edmica i Biotecnologia. Grup Alimentaci\u00f3, Nutrici\u00f3, Desenvolupament i Salut Mental (ANUT\u2010DSM) Universitat Rovira i Virgili  Reus Spain"},{"name":"Centro De Investigaci\u00f3n Biom\u00e9dica En Red de Fisiopatolog\u00eda de La Obesidad y Nutrici\u00f3n (CIBEROBN) Instituto De Salud Carlos III  Madrid Spain"},{"name":"Institut de Recerca Biom\u00e8dica Catalunya Sud (IRBCatSud), former IISPV  Reus Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carles","family":"Arribas","sequence":"additional","affiliation":[{"name":"Genomics Unit Josep Carreras Leukaemia Research Institute (IJC)  Badalona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4490-6093","authenticated-orcid":false,"given":"Manel","family":"Esteller","sequence":"additional","affiliation":[{"name":"Institut de Recerca de Sant Pau Cancer Epigenetics Group  Barcelona Spain"},{"name":"Centro de Investigacion Biomedica en Red Cancer (CIBERONC) Instituto de Salud Carlos III  Madrid Spain"},{"name":"Institucio Catalana de Recerca i Estudis Avan\u00e7ats (ICREA)  Barcelona Spain"},{"name":"Physiological Sciences Department, School of Medicine and Health Sciences University of Barcelona (UB)  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7436-6513","authenticated-orcid":false,"given":"Aleix","family":"Noguera\u2010Castells","sequence":"additional","affiliation":[{"name":"Centro de Investigacion Biomedica en Red Cancer (CIBERONC) Instituto de Salud Carlos III  Madrid Spain"},{"name":"Josep Carreras Leukemia Research Institute (IJC) Cancer Epigenetics Group  Badalona Spain"},{"name":"Department of Biosciences, Faculty of Science, Technology and Engineering University of Vic, Central University of Catalonia (UVic\u2010UCC)  Vic Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4955-8238","authenticated-orcid":false,"given":"Raquel","family":"Pluvinet","sequence":"additional","affiliation":[{"name":"Genomics Unit Josep Carreras Leukaemia Research Institute (IJC)  Badalona Spain"},{"name":"High Content Genomics and Bioinformatics Facility Germans Trias i Pujol Research Institute (IGTP)  Badalona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2700-7459","authenticated-orcid":false,"given":"Jordi","family":"Salas\u2010Salvad\u00f3","sequence":"additional","affiliation":[{"name":"Unitat de Nutrici\u00f3 Humana. Departament de Bioqu\u00edmica i Biotecnologia. Grup Alimentaci\u00f3, Nutrici\u00f3, Desenvolupament i Salut Mental (ANUT\u2010DSM) Universitat Rovira i Virgili  Reus Spain"},{"name":"Centro De Investigaci\u00f3n Biom\u00e9dica En Red de Fisiopatolog\u00eda de La Obesidad y Nutrici\u00f3n (CIBEROBN) Instituto De Salud Carlos III  Madrid Spain"},{"name":"Institut de Recerca Biom\u00e8dica Catalunya Sud (IRBCatSud), former IISPV  Reus Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5914-6862","authenticated-orcid":false,"given":"Albert","family":"Salas\u2010Huetos","sequence":"additional","affiliation":[{"name":"Centro De Investigaci\u00f3n Biom\u00e9dica En Red de Fisiopatolog\u00eda de La Obesidad y Nutrici\u00f3n (CIBEROBN) Instituto De Salud Carlos III  Madrid Spain"},{"name":"Institut de Recerca Biom\u00e8dica Catalunya Sud (IRBCatSud), former IISPV  Reus Spain"},{"name":"Unit of Preventive Medicine and Biostatistics. Departament de Ci\u00e8ncies M\u00e8diques B\u00e0siques. Grup Alimentaci\u00f3, Nutrici\u00f3, Desenvolupament i Salut Mental (ANUT\u2010DSM) Universitat Rovira i Virgili  Reus Spain"},{"name":"Department of Nutrition Harvard T.H. Chan School of Public Health  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,29]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12986\u2010024\u201000814\u2010y"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43016\u2010024\u201001059\u2010x"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/nutrit\/nuac087"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2009.12.079"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/nejmoa1800389"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40520\u2010024\u201002718\u20106"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.13789"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.euf.2021.01.017"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crfs.2023.100636"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.advnut.2025.100454"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3889"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dew308"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2021.09.009"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12263\u2010023\u201000731\u20104"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2021.633943"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12911"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/hropen\/hoae001"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114509993837"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.3945\/jn.110.135566"},{"key":"e_1_2_11_21_1","volume-title":"WHO Laboratory Manual for the Examination and Processing of Human Semen Sixth Edition","author":"WHO","year":"2021"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12231"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu049"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1080\/15592294.2023.2185742"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt090"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw377"},{"key":"e_1_2_11_27_1","volume-title":"WHO Laboratory Manual for the Examination and Processing of Human Semen, 5th ed","author":"WHO","year":"2010"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059\u2010017\u20101363\u20103"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.csbj.2025.11.060"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.24418"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.18502\/ijrm.v20i10.12265"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12958\u2010017\u20100281\u20102"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.15761\/IFNM.1000308"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/nutrit\/nuz094"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13148\u2010015\u20100144\u20107"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu14040908"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2021.108839"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nut.2021.111340"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.3945\/an.111.000992"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13148\u2010023\u201001578\u20107"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12416"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2022.118994"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2024.10.010"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13148\u2010023\u201001541\u20106"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2011.07.007"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815\u2010024\u201003202\u2010w"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.3389\/frph.2025.1506117"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.3389\/fragi.2024.1417625"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2023.155594"}],"container-title":["Andrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/andr.70398","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/andr.70398","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/andr.70398","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T07:47:18Z","timestamp":1790668038000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/andr.70398"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,29]]},"references-count":48,"alternative-id":["10.1111\/andr.70398"],"URL":"https:\/\/doi.org\/10.1111\/andr.70398","archive":["Portico"],"relation":{},"ISSN":["2047-2919","2047-2927"],"issn-type":[{"value":"2047-2919","type":"print"},{"value":"2047-2927","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,29]]},"assertion":[{"value":"2026-03-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"andr.70398"}}