{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T09:44:51Z","timestamp":1792143891488,"version":"4.4.0"},"reference-count":35,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:00:00Z","timestamp":1792108800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:00:00Z","timestamp":1792108800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Andrology"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Uncertainty persists regarding the impact of very long\u2010term liquid nitrogen storage (&gt;10 years) on sperm quality, particularly for oncology patients and those with suboptimal initial semen quality.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To evaluate the impact of storage duration on post\u2010thaw motility parameters and clinical assisted reproductive technologies (ART) utility using a robust paired\u2010sample design.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      This paired cohort study analyzed 101 semen samples (62 cancer, 39 non\u2010cancer) at two time points: immediately after freezing (\n                      <jats:italic>T<\/jats:italic>\n                      0) and after 2\u201312 years of storage (\n                      <jats:italic>T<\/jats:italic>\n                      1) in 0.3\u2010mL high\u2010security straws. Total\/progressive motility and motile sperm counts (TMSC\/PMSC) were compared using random\u2010effects modeling with interaction terms for patient subgroups.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Sperm parameters showed minimal average change over the 2\u201312\u2010year storage range, with no evidence of differential\n                      <jats:italic>T<\/jats:italic>\n                      0\u2013\n                      <jats:italic>T<\/jats:italic>\n                      1 variation according to initial sperm quality or diagnosis (all interaction\n                      <jats:italic>p<\/jats:italic>\n                      values &gt;0.3). An exploratory analysis of the small 11\u201312\u2010year subgroup (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a024; including 21 oncology patients) showed a significant but modest decline in total motility (\u22123.6%;\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05) and motile counts (TMSC \u22120.5\u00a0M;\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05). Concordance coefficients provided supporting information on relative agreement between\n                      <jats:italic>T<\/jats:italic>\n                      0 and\n                      <jats:italic>T<\/jats:italic>\n                      1 (Lin's\u00a0&gt;\u00a00.70;\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.001), and most samples (84.6% overall, 87.5% in the 11\u201312\u2010year group) maintained their original ART eligibility (intrauterine insemination [IUI]\/in vitro fertilization [IVF]\/intracytoplasmic sperm injection [ICSI]).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion and Conclusion<\/jats:title>\n                    <jats:p>Human sperm motility showed limited average changes over the 2\u201312\u2010year storage range. The exploratory findings in the small 11\u201312\u2010year subgroup, predominantly composed of oncology patients, suggest modest changes with limited impact on ART strategy. These results provide reassuring evidence for long\u2010term counseling in oncofertility and support the clinical management of cryopreserved sperm samples.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/andr.70414","type":"journal-article","created":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T08:54:07Z","timestamp":1792140847000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Sperm Motility Stability During Long\u2010Term Cryopreservation: A Paired\u2010Sample Study on 101 Fertility Preservation Patients"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4276-723X","authenticated-orcid":false,"given":"C\u00e9cily","family":"Lucas","sequence":"first","affiliation":[{"name":"CHU Clermont Ferrand, CHU Estaing, Assistance M\u00e9dicale \u00e0 la Procr\u00e9ation CECOS  Clermont\u2010Ferrand 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Estaing, Assistance M\u00e9dicale \u00e0 la Procr\u00e9ation CECOS  Clermont\u2010Ferrand France"},{"name":"Facult\u00e9 de M\u00e9decine IMoST INSERM 1240 Universit\u00e9 Clermont Auvergne  Clermont\u2010Ferrand France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cyril","family":"Bouche","sequence":"additional","affiliation":[{"name":"CHU Clermont Ferrand, CHU Estaing, Assistance M\u00e9dicale \u00e0 la Procr\u00e9ation CECOS  Clermont\u2010Ferrand France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Brugnon","sequence":"additional","affiliation":[{"name":"CHU Clermont Ferrand, CHU Estaing, Assistance M\u00e9dicale \u00e0 la Procr\u00e9ation CECOS  Clermont\u2010Ferrand France"},{"name":"Facult\u00e9 de M\u00e9decine IMoST INSERM 1240 Universit\u00e9 Clermont Auvergne  Clermont\u2010Ferrand France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9779-9693","authenticated-orcid":false,"given":"Hanae","family":"Pons\u2010Rejraji","sequence":"additional","affiliation":[{"name":"CHU Clermont Ferrand, CHU Estaing, Assistance M\u00e9dicale \u00e0 la Procr\u00e9ation CECOS  Clermont\u2010Ferrand France"},{"name":"Facult\u00e9 de M\u00e9decine IMoST INSERM 1240 Universit\u00e9 Clermont Auvergne  Clermont\u2010Ferrand 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