{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T08:58:28Z","timestamp":1789894708703,"version":"4.0.1"},"reference-count":20,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2013,10,31]],"date-time":"2013-10-31T00:00:00Z","timestamp":1383177600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Garnett Passe and Rodney Williams Memorial Foundation Grant"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ANZ Journal of Surgery"],"published-print":{"date-parts":[[2015,11]]},"abstract":"Abstract<\/jats:title>Objective<\/jats:title>The survival rate for head and neck squamous cell carcinoma (HNSCC<\/jats:styled-content>) is among the lowest of the major cancers and has not substantially improved in the past two decades. Tumours with similar histological features may have widely differing clinical outcomes and thus identification of prognostic and predictive biomarkers may be valuable for determining appropriate clinical management strategies. The objective of this study was to establish the prognostic significance of six molecular markers in HNSCC<\/jats:styled-content> in a N<\/jats:styled-content>ew Z<\/jats:styled-content>ealand population: matrix metalloproteinases 2 and 9 (MMP<\/jats:styled-content>\u20102, MMP<\/jats:styled-content>\u20109), tissue inhibitor of matrix metalloproteinase\u20101, sialyl L<\/jats:styled-content>ewis antigens a and x (sLe<\/jats:styled-content>a<\/jats:sup>, sLe<\/jats:styled-content>x<\/jats:sup>) and alpha B<\/jats:styled-content>\u2010crystallin.<\/jats:p><\/jats:sec>Methods<\/jats:title>Retrospective review of 145 sequential HNSCC<\/jats:styled-content> patients from a tertiary centre with minimum 3 years surveillance. Sections from formalin\u2010fixed paraffin\u2010embedded tumour blocks were immunostained for the molecular markers and scored. C<\/jats:styled-content>ox regression modelling was used to adjust for potential confounding variables impacting on cancer survival.<\/jats:p><\/jats:sec>Results<\/jats:title>Multivariate analysis for individual biomarkers, controlling for age, sex, tumour grade, N<\/jats:styled-content>\u2010stage, T<\/jats:styled-content>\u2010stage, tumour site, smoking history and alcohol use, revealed poorer survival with tumour expression of MMP<\/jats:styled-content>\u20102 (hazard ratio = 1.98, 95% confidence interval: 1.11\u20133.52, P<\/jats:italic> = 0.021) and sLe<\/jats:styled-content>x<\/jats:sup> (hazard ratio = 3.22, 95% confidence interval: 1.33\u20137.80, P<\/jats:italic> = 0.010). A stepwise analysis showed that MMP<\/jats:styled-content>\u20102 and sLe<\/jats:styled-content>x<\/jats:sup> were independently prognostic after covariate adjustment.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>MMP<\/jats:styled-content>\u20102 and sLe<\/jats:styled-content>x<\/jats:sup> were negative prognostic markers for survival in these HNSCC<\/jats:styled-content> patients. This offers opportunities for clinical trials to reduce the risk of nodal and distant metastases through blocking tumour cell adhesion to endothelium.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ans.12424","type":"journal-article","created":{"date-parts":[[2013,10,31]],"date-time":"2013-10-31T11:13:15Z","timestamp":1383217995000},"page":"843-848","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Prognostic molecular markers in head and neck squamous cell carcinoma in a N<\/scp>ew Z<\/scp>ealand population: matrix metalloproteinase\u20102 and sialyl L<\/scp>ewis x antigen"],"prefix":"10.1111","volume":"85","author":[{"given":"Indunil","family":"Gunawardena","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Waikato Hospital Hamilton New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Arendse","sequence":"additional","affiliation":[{"name":"Department of Pathology Waikato Hospital Hamilton New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael B.","family":"Jameson","sequence":"additional","affiliation":[{"name":"Regional Cancer Centre Waikato Hospital Hamilton New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lindsay D.","family":"Plank","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Auckland Auckland New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. Theo","family":"Gregor","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Waikato Hospital Hamilton New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,10,31]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0959-9673.2005.00447.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1758-3284-2-15"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ypmed.2011.08.001"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0347(200102)23:2<147::AID-HED1010>3.0.CO;2-#"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjps.2004.12.025"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-03-0530"},{"key":"e_1_2_6_8_1","first-page":"347","article-title":"Human colon cancer cells express multiple glycoprotein ligands for E\u2010selectin","volume":"16","author":"Tomlinson J","year":"2000","journal-title":"Int. J. Oncol."},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1018532409041"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.MLG.0000164715.86240.55"},{"key":"e_1_2_6_11_1","first-page":"813","article-title":"Expression of matrix metalloproteinases and their inhibitors correlates with invasion and metastasis in squamous cell carcinoma of the head and neck","volume":"127","author":"O\u2010Charoenrat P","year":"2001","journal-title":"Arch. Otolaryngol. Head Neck Surg."},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03032385"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0347(199907)21:4<330::AID-HED7>3.0.CO;2-#"},{"key":"e_1_2_6_14_1","first-page":"991","article-title":"High serum soluble E\u2010selectin levels are associated with postoperative haematogenic recurrence in esophageal squamous cell carcinoma patients","volume":"10","author":"Shimada Y","year":"2003","journal-title":"Oncol. Rep."},{"key":"e_1_2_6_15_1","first-page":"1427","article-title":"Prognostic value of circulating soluble E\u2010selectin concentrations in node\u2010negative breast cancer patients","volume":"5","author":"Hebbar M","year":"1999","journal-title":"Clin. Cancer Res."},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.4513"},{"key":"e_1_2_6_17_1","first-page":"3978","article-title":"Cimetidine inhibits cancer cell adhesion to endothelial cells and prevents metastasis by blocking E\u2010selectin expression","volume":"60","author":"Kobayashi K","year":"2000","journal-title":"Cancer Res."},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1538-7836.2003.00412.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(88)90743-X"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6600048"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD007814.pub2"}],"container-title":["ANZ Journal of Surgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fans.12424","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ans.12424","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T15:12:27Z","timestamp":1695568347000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ans.12424"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,10,31]]},"references-count":20,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2015,11]]}},"alternative-id":["10.1111\/ans.12424"],"URL":"https:\/\/doi.org\/10.1111\/ans.12424","archive":["Portico"],"relation":{},"ISSN":["1445-1433","1445-2197"],"issn-type":[{"value":"1445-1433","type":"print"},{"value":"1445-2197","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,10,31]]},"assertion":[{"value":"2013-09-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-10-31","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}