{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T00:00:44Z","timestamp":1768262444298,"version":"3.49.0"},"reference-count":26,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2014,1,8]],"date-time":"2014-01-08T00:00:00Z","timestamp":1389139200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"All equipment and reagents required for the OSNA BC assay were supplied by Sysmex"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ANZ Journal of Surgery"],"published-print":{"date-parts":[[2014,10]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>Intra\u2010operative assessment of sentinel lymph nodes in breast cancer offers the opportunity to prevent two\u2010stage surgical procedures. At our institution we employ touch imprint cytology (TIC<\/jats:styled-content>), which lacks sensitivity. In this study we compare the one\u2010step nucleic acid amplification (OSNA<\/jats:styled-content>) assay to TIC<\/jats:styled-content>.<\/jats:p><\/jats:sec>Methods<\/jats:title>Imprints were taken from 63 lymph nodes from 35 patients. The lymph nodes were sectioned at 2\u2010mm intervals and alternate slices submitted for either histology or OSNA<\/jats:styled-content> assay, with histology as the reference standard.<\/jats:p><\/jats:sec>Results<\/jats:title>Seven patients were histologically positive. Nine and five patients were positive by OSNA<\/jats:styled-content> and TIC<\/jats:styled-content>, respectively. Sensitivity, specificity, positive and negative predictive value for the OSNA<\/jats:styled-content> assay were 85.7%, 85.7%, 63.6% and 96.6% and for TIC<\/jats:styled-content> were 70.0%, 96.6%, 87.5% and 90.3%.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>In this study OSNA<\/jats:styled-content> had a higher sensitivity than TIC<\/jats:styled-content>. Fewer patients assessed by the OSNA<\/jats:styled-content> assay would have required a two\u2010stage procedure. The OSNA<\/jats:styled-content> assay appears to be a highly cost\u2010effective method for providing rapid and reliable intra\u2010operative assessment of sentinel lymph nodes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ans.12497","type":"journal-article","created":{"date-parts":[[2014,1,8]],"date-time":"2014-01-08T06:30:50Z","timestamp":1389162650000},"page":"725-729","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Is the one\u2010step nucleic acid amplification assay better for intra\u2010operative assessment of breast sentinel nodes?"],"prefix":"10.1111","volume":"84","author":[{"given":"Mark","family":"Bettington","sequence":"first","affiliation":[{"name":"Royal Brisbane and Women's Hospital The University of Queensland Brisbane Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sunil R.","family":"Lakhani","sequence":"additional","affiliation":[{"name":"Royal Brisbane and Women's Hospital The University of Queensland Brisbane Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Owen A.","family":"Ung","sequence":"additional","affiliation":[{"name":"Royal Brisbane and Women's Hospital The University of Queensland Brisbane Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,1,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(06)70947-0"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(97)01004-0"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199810013391401"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2011.90"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(13)70035-4"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.breast.2011.09.013"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(20010915)92:6<1378::AID-CNCR1460>3.0.CO;2-Y"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.0b013e31823dc616"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.26291"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.23451"},{"key":"e_1_2_7_12_1","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1097\/00129039-200303000-00008","article-title":"Rapid immunohistochemistry for cytokeratin in the intraoperative evaluation of sentinel lymph nodes for metastatic breast carcinoma","volume":"11","author":"Beach RA","year":"2003","journal-title":"Appl. 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