{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T05:56:39Z","timestamp":1757310999012,"version":"3.37.3"},"reference-count":20,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,12,14]],"date-time":"2017-12-14T00:00:00Z","timestamp":1513209600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100011371","name":"Royal Australasian College of Surgeons","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011371","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ANZ Journal of Surgery"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The ongoing supply\u2010demand gap with respect to donor kidneys for transplantation necessitates the increased use of higher kidney donor profile index and\/or donation after circulatory death (DCD) kidneys. Machine perfusion (MP) preservation has become increasingly popular as a means to preserve such organs. Human data regarding normothermic kidney MP (NMP) is in its infancy, and such a system has not been established in the Australasian clinical setting.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Modified cardio\u2010pulmonary bypass technology was utilized to develop a viable NMP kidney perfusion system using a porcine DCD model. System development and optimization occurred in two stages, with system components added in each experiment to identify optimal perfusion conditions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Device functionality was demonstrated by the successful perfusion of and urine production by, eight porcine kidneys. Urine production diminished in the presence of colloid in the perfusate. Pressure\u2010controlled (compared with flow\u2010controlled) perfusion is preferable as a safe perfusion pressure range can be maintained. More physiologic perfusion conditions are achieved if oxygenation is provided by an oxygen\/carbon dioxide mixture compared to 100% oxygen.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>A viable and reproducible NMP system was established and tested in porcine kidneys, which was able to simulate graft function extra\u2010corporeally. Further work is required to identify the most optimal perfusion conditions. Prior to its utilization in clinical transplantation, the system should be tested in non\u2010transplanted human kidneys.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ans.14321","type":"journal-article","created":{"date-parts":[[2017,12,14]],"date-time":"2017-12-14T05:44:42Z","timestamp":1513230282000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Extra\u2010corporeal normothermic machine perfusion of the porcine kidney: working towards future utilization in Australasia"],"prefix":"10.1111","volume":"88","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2625-6939","authenticated-orcid":false,"given":"Ahmer M.","family":"Hameed","sequence":"first","affiliation":[{"name":"Department of Surgery Westmead Hospital  Sydney New South Wales Australia"},{"name":"Centre for Transplant and Renal Research Westmead Institute for Medical Research  Sydney New South Wales Australia"},{"name":"Sydney Medical School The University of Sydney  Sydney New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ray","family":"Miraziz","sequence":"additional","affiliation":[{"name":"Department of Anaesthesia Westmead Hospital  Sydney New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David B.","family":"Lu","sequence":"additional","affiliation":[{"name":"Centre for Transplant and Renal Research Westmead Institute for Medical Research  Sydney New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neil","family":"Warwick","sequence":"additional","affiliation":[{"name":"Department of Anaesthesia Westmead Hospital  Sydney New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ali","family":"El\u2010Ayoubi","sequence":"additional","affiliation":[{"name":"Centre for Transplant and Renal Research Westmead Institute for Medical Research  Sydney New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heather","family":"Burns","sequence":"additional","affiliation":[{"name":"Centre for Transplant and Renal Research Westmead Institute for Medical Research  Sydney New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi Vee","family":"Chew","sequence":"additional","affiliation":[{"name":"Centre for Transplant and Renal Research Westmead Institute for Medical Research  Sydney New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ross","family":"Matthews","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences Westmead Hospital  Sydney New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Greg","family":"O'Grady","sequence":"additional","affiliation":[{"name":"Department of Surgery The University of Auckland  Auckland New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lawrence","family":"Yuen","sequence":"additional","affiliation":[{"name":"Department of Surgery Westmead Hospital  Sydney New South Wales Australia"},{"name":"Sydney Medical School The University of Sydney  Sydney New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natasha","family":"Rogers","sequence":"additional","affiliation":[{"name":"Centre for Transplant and Renal Research Westmead Institute for Medical Research  Sydney New South Wales Australia"},{"name":"Sydney Medical School The University of Sydney  Sydney New South Wales Australia"},{"name":"Department of Renal Medicine Westmead Hospital  Sydney New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henry C.","family":"Pleass","sequence":"additional","affiliation":[{"name":"Department of Surgery Westmead Hospital  Sydney New South Wales Australia"},{"name":"Sydney Medical School The University of Sydney  Sydney New South Wales Australia"},{"name":"Department of Surgery Royal Prince Alfred Hospital  Sydney New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wayne J.","family":"Hawthorne","sequence":"additional","affiliation":[{"name":"Department of Surgery Westmead Hospital  Sydney New South Wales Australia"},{"name":"Centre for Transplant and Renal Research Westmead Institute for Medical Research  Sydney New South Wales Australia"},{"name":"Sydney Medical School The University of Sydney  Sydney New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,12,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2011.03741.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2007.01993.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000001323"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0012.2010.01241.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/ans.13713"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000005083"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.9894"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.12179"},{"key":"e_1_2_7_10_1","unstructured":"TSANZ Annual Newsletter 2016 [PDF on Internet]. Sydney: TSANZ. [Cited 15 Aug 2017.] Available from URL:https:\/\/www.tsanz.com.au\/documents\/2016Newsletter.pdf"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.14059"},{"issue":"101","key":"e_1_2_7_12_1","first-page":"e52909","article-title":"Normothermic ex vivo kidney perfusion for the preservation of kidney grafts prior to transplantation","author":"Kaths JM","year":"2015","journal-title":"J. Vis. Exp."},{"issue":"108","key":"e_1_2_7_13_1","first-page":"53765","article-title":"Heterotopic renal autotransplantation in a porcine model: a step\u2010by\u2010step protocol","author":"Kaths JM","year":"2016","journal-title":"J. Vis. Exp."},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000001084"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1159\/000366155"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2014.04.003"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.5381"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/TXD.0000000000000655"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.109.10.2571"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.14294"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2016-012237"}],"container-title":["ANZ Journal of Surgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fans.14321","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ans.14321","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T18:45:28Z","timestamp":1695494728000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ans.14321"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12,14]]},"references-count":20,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2018,5]]}},"alternative-id":["10.1111\/ans.14321"],"URL":"https:\/\/doi.org\/10.1111\/ans.14321","archive":["Portico"],"relation":{},"ISSN":["1445-1433","1445-2197"],"issn-type":[{"type":"print","value":"1445-1433"},{"type":"electronic","value":"1445-2197"}],"subject":[],"published":{"date-parts":[[2017,12,14]]},"assertion":[{"value":"2017-09-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-11-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-12-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}