{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T10:08:58Z","timestamp":1772186938489,"version":"3.50.1"},"reference-count":17,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,12,5]],"date-time":"2012-12-05T00:00:00Z","timestamp":1354665600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Obstet Gynecol Scand"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Objective.<\/jats:italic> To evaluate enzyme\u2010linked immunosorbent assay (ELISA) for cyclin\u2010dependent kinase inhibitor 2A protein (p16<jats:sup>INK4a<\/jats:sup>) on self\u2010collected cervicovaginal lavage samples as an additional triage test to identify women with high\u2010grade cervical intraepithelial neoplasia (CIN). <jats:italic>Design.<\/jats:italic> Retrospective feasibility, sensitivity and specificity study. <jats:italic>Setting.<\/jats:italic> University Medical School, Germany. <jats:italic>Sample.<\/jats:italic> One hundred and fifty\u2010two patients from the colposcopy clinic were included. <jats:italic>Methods.<\/jats:italic> All women used a cervico\u2010vaginal lavage device (Delphi Screener) for self\u2010sampling and had gynecological examinations with Pap smears, cervical smears in ThinPrep PreservCyt solution and Cervatec medium for human papillomavirus (HPV) testing (Qiagen Hybrid Capture 2) and colposcopic examinations with biopsies if abnormalities were detected (72 women; 51%). All cytological samples were examined by p16<jats:sup>INK4a<\/jats:sup> ELISA. <jats:italic>Main outcome measures.<\/jats:italic> Sensitivity and specificity of p16<jats:sup>INK4a<\/jats:sup> ELISA for high\u2010grade CIN. <jats:italic>Results.<\/jats:italic> Complete data were available for 140 women. Among these, 62 women (46%) presented with an atypical Pap smear and 65 (46.4%) were high\u2010risk HPV positive in the reference smear sample. Seventeen women (12%) had CIN 3+. Twenty\u2010seven (19%) physician\u2010collected samples were p16<jats:sup>INK4a<\/jats:sup> ELISA positive. In contrast, p16<jats:sup>INK4a<\/jats:sup> ELISA turned out to be positive in only one (1%) vaginal lavage sample. <jats:italic>Conclusions.<\/jats:italic> Our study shows that self\u2010sampling with cervicovaginal lavage followed by p16<jats:sup>INK4a<\/jats:sup> ELISA is not suitable for the detection of high\u2010grade CIN.<\/jats:p>","DOI":"10.1111\/aogs.12032","type":"journal-article","created":{"date-parts":[[2012,10,17]],"date-time":"2012-10-17T09:03:01Z","timestamp":1350464581000},"page":"160-164","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Enzyme\u2010linked immunosorbent assay for p16<sup>INK4a<\/sup>\u2013 a new triage test for the detection of cervical intraepithelial neoplasia?"],"prefix":"10.1111","volume":"92","author":[{"given":"MATTHIAS","family":"JENTSCHKE","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"VICTORIA","family":"LANGE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"PHILIPP","family":"SOERGEL","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"PETER","family":"HILLEMANNS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,12,5]]},"reference":[{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.c1040"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02440-05"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(99)04110-0"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.21955"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.9.4350"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1997.243"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(199909)189:1<12::AID-PATH431>3.0.CO;2-F"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1998.16.3.1197"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1006\/gyno.1998.5041"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1309\/AJCP3CD9YKYFJDQL"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.22612"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.22247"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-09-0328"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(87)92695-X"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djn099"},{"key":"e_1_2_8_17_2","doi-asserted-by":"publisher","DOI":"10.1159\/000331811"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(20000515)88:10<2283::AID-CNCR12>3.0.CO;2-D"}],"container-title":["Acta Obstetricia et Gynecologica Scandinavica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Faogs.12032","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aogs.12032","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T12:47:08Z","timestamp":1698670028000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1111\/aogs.12032"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,12,5]]},"references-count":17,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2013,2]]}},"alternative-id":["10.1111\/aogs.12032"],"URL":"https:\/\/doi.org\/10.1111\/aogs.12032","archive":["Portico"],"relation":{},"ISSN":["0001-6349","1600-0412"],"issn-type":[{"value":"0001-6349","type":"print"},{"value":"1600-0412","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,12,5]]},"assertion":[{"value":"2012-12-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}