{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T13:53:49Z","timestamp":1722606829652},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,1,17]],"date-time":"2014-01-17T00:00:00Z","timestamp":1389916800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Obstet Gynecol Scand"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>New human papillomavirus (<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>)\u2010related biomarkers may allow better identification of clinically significant lesions that warrant excision and, conversely, identification of the false positive cases that have been overreported by traditional techniques. The aim of this study was to investigate how the expression of several <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>\u2010related biomarkers correlates to the severity of the lesion at treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>Prospective observational study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting<\/jats:title><jats:p>University Hospital (2009\u20132011).<\/jats:p><\/jats:sec><jats:sec><jats:title>Population<\/jats:title><jats:p>All women submitted for excisional treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A liquid\u2010based cytology sample was obtained before treatment and was tested for HPV typing, <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> E6 &amp; E7 with NASBA or flow cytometry and p16. All women had histological diagnosis in the form of excisional cone (gold standard).<\/jats:p><\/jats:sec><jats:sec><jats:title>Main outcome measures<\/jats:title><jats:p>Correlation of <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> biomarker positivity rates to the grade of the lesion at treatment histology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Two hundred women were recruited: 23 were found to have negative histology (11.5%), 79 (39.5%) CIN1, 50 (25.0%) CIN2 and 48 (24.0%) CIN3. All biomarkers (HPV DNA typing, HR HPV, single HPV 16\/18, <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> E6 &amp; E7 expression and p16) revealed an increased linear positivity rate with increasing severity and grade of the lesion (chi\u2010squared test for trend <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). This was stronger for HPV (all and high\u2010risk) followed by <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> with NASBA, flow cytometry, HPV 16\/18 and ultimately p16 immunostaining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The linear correlation between various <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>\u2010related biomarkers and the grade of the lesion suggests that these biomarkers may prove to be useful in the prediction of <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content> grade and, as a result, the need for treatment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aogs.12298","type":"journal-article","created":{"date-parts":[[2013,10,31]],"date-time":"2013-10-31T16:36:00Z","timestamp":1383237360000},"page":"194-200","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Expression of <scp>HPV<\/scp>\u2010related biomarkers and grade of cervical intraepithelial lesion at treatment"],"prefix":"10.1111","volume":"93","author":[{"given":"George","family":"Valasoulis","sequence":"first","affiliation":[{"name":"Department of Obstetrics &amp; Gynaecology \u2013 Gynaecologic Oncology University Hospital of Ioannina Ioannina Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sofia\u2010Melina","family":"Stasinou","sequence":"additional","affiliation":[{"name":"Department of Obstetrics &amp; Gynaecology \u2013 Gynaecologic Oncology University Hospital of Ioannina Ioannina Greece"},{"name":"IVF Unit Queen Charlotte's &amp; Chelsea \u2013 Hammersmith Hospital Imperial Healthcare NHS Trust London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Nasioutziki","sequence":"additional","affiliation":[{"name":"Department of Cytopathology University of Thessaloniki Thessaloniki Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonis","family":"Athanasiou","sequence":"additional","affiliation":[{"name":"Department of Obstetrics &amp; Gynaecology \u2013 Gynaecologic Oncology University Hospital of Ioannina Ioannina Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Zografou","sequence":"additional","affiliation":[{"name":"Department of Obstetrics &amp; Gynaecology \u2013 Gynaecologic Oncology University Hospital of Ioannina Ioannina Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aris","family":"Spathis","sequence":"additional","affiliation":[{"name":"Department of Cytopathology Attikon Hospital University of Athens Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aristotelis","family":"Loufopoulos","sequence":"additional","affiliation":[{"name":"Department of Obstetrics &amp; Gynaecology University of Thessaloniki Thessaloniki Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petros","family":"Karakitsos","sequence":"additional","affiliation":[{"name":"Department of Cytopathology Attikon Hospital University of Athens Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evangelos","family":"Paraskevaidis","sequence":"additional","affiliation":[{"name":"Department of Obstetrics &amp; Gynaecology \u2013 Gynaecologic Oncology University Hospital of Ioannina Ioannina Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Kyrgiou","sequence":"additional","affiliation":[{"name":"West London Gynaecological Cancer Centre Queen Charlotte's &amp; Chelsea \u2013 Hammersmith Hospital Imperial Healthcare NHS Trust London UK"},{"name":"Department of Surgery &amp; Cancer Faculty of Medicine Imperial College London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,1,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2009.12.014"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.25516"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(199909)189:1<12::AID-PATH431>3.0.CO;2-F"},{"key":"e_1_2_8_5_1","first-page":"1141","article-title":"Audit shows weaknesses in cervical cancer screening","volume":"322","author":"Ferriman A","year":"2001","journal-title":"Br Med J"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-0528.2012.03444.x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctrv.2007.05.002"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djh037"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2010.05676.x"},{"key":"e_1_2_8_10_1","unstructured":"The NHS Information Centre Screening and Immunisations team.2011. 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