{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T20:03:31Z","timestamp":1762459411178},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,11,9]],"date-time":"2016-11-09T00:00:00Z","timestamp":1478649600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Helsinki University Hospital Laboratory"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Obstet Gynecol Scand"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>The aim was to assess the performance of two commercial assays for the detection of high\u2010risk human papillomavirus (hr<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>): Aptima <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> Assay (Hologic, Inc., Marlborough, <jats:styled-content style=\"fixed-case\">MA<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> USA<\/jats:styled-content>) which detects <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> of 14 different hr<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> types, and Hybrid Capture 2 <jats:styled-content style=\"fixed-case\">HPV DNA<\/jats:styled-content> test (<jats:styled-content style=\"fixed-case\">HC<\/jats:styled-content>2; Qiagen, Gaithersburg, <jats:styled-content style=\"fixed-case\">MD<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> USA<\/jats:styled-content>), which detects the <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> of 13 different hr<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> types. Test performance was compared in the settings of a standard colposcopy clinic, among the regular patient flow.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>Two separate cervical cell samples for Aptima and <jats:styled-content style=\"fixed-case\">HC<\/jats:styled-content>2 testing were collected from women referred to colposcopy or a cervical follow\u2010up visit. Altogether, 481 paired samples were analyzed and all positive samples were also tested using the Aptima <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> 16 18\/45 Genotype Assay. Results from the two assays were compared directly and with stratification by histology and cytology from the same sampling visit.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The overall agreement between <jats:styled-content style=\"fixed-case\">HC<\/jats:styled-content>2 and Aptima assays was 92.9% (Kappa coefficient of 0.855). The sensitivity and specificity of the assays in detecting <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>2<jats:sup>+<\/jats:sup> were 92.5 and 58.2% for <jats:styled-content style=\"fixed-case\">HC<\/jats:styled-content>2, and 94.0 and 59.3% for Aptima, respectively. No significant differences between the assays were found (<jats:italic>p<\/jats:italic>\u2010values &gt;0.5). Both assays detected all <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>3 (<jats:italic>n\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>30) and carcinoma (<jats:italic>n\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>2) cases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>\u2010based Aptima assay and the extensively studied <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\u2010based <jats:styled-content style=\"fixed-case\">HC<\/jats:styled-content>2 test performed equally well in detecting high\u2010grade cervical lesions. Our data contribute to the growing evidence base indicating that the <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>\u2010based Aptima assay could be used for the triage of patients with <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>\u2010associated cervical disease.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aogs.13041","type":"journal-article","created":{"date-parts":[[2016,10,14]],"date-time":"2016-10-14T02:26:19Z","timestamp":1476411979000},"page":"61-68","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Performance of <scp>mRNA<\/scp>\u2010 and <scp>DNA<\/scp>\u2010based high\u2010risk human papillomavirus assays in detection of high\u2010grade cervical lesions"],"prefix":"10.1111","volume":"96","author":[{"given":"Elina","family":"Virtanen","sequence":"first","affiliation":[{"name":"Department of Virology University of Helsinki and Helsinki University Hospital Helsinki 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