{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T05:32:27Z","timestamp":1783488747539,"version":"3.55.0"},"reference-count":30,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,4,13]],"date-time":"2019-04-13T00:00:00Z","timestamp":1555113600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Obstet Gynecol Scand"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>There is now sufficient evidence to support the feasibility and efficacy of ovarian tissue (OT) cryopreservation and transplantation for both fertility preservation and restoration purposes. However, there are still issues to address regarding the grafting procedure itself, since transplanted tissue suffers massive follicle loss in the early post\u2010grafting period. To improve follicle survival after transplantation, our group recently developed a two\u2010step transplantation technique for OT transplantation in a xenografting model using adipose tissue\u2010derived stem cells (<jats:styled-content style=\"fixed-case\">ASC<\/jats:styled-content>s). The aim of this narrative review is to describe and discuss the previously reported findings. <jats:styled-content style=\"fixed-case\">ASC<\/jats:styled-content>s were initially characterized by flow cytometry as positive for <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>29, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>44, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>73, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>90, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>105 and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>166 (&gt;95%) and negative for <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>34, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>14, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>31, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>45 and Lin1. <jats:styled-content style=\"fixed-case\">ASC<\/jats:styled-content>s were used in a model of xenotransplantation, were they were embedded in a fibrin scaffold and transplanted to the peritoneum of immunodeficient mice. The goal of the first step was to increase levels of partial pressure of oxygen (<jats:styled-content style=\"fixed-case\">pO<\/jats:styled-content><jats:sub>2<\/jats:sub>) and revascularization in the peritoneal transplantation site for further OT transplantation. As <jats:styled-content style=\"fixed-case\">ASC<\/jats:styled-content>s showed the ability to differentiate into endothelial\u2010like cells and vessels in our model, OT transplantation was then performed with <jats:styled-content style=\"fixed-case\">ASC<\/jats:styled-content> grafts in a controlled experiment. At 7\u00a0days post\u2010transplantation, the <jats:styled-content style=\"fixed-case\">ASC<\/jats:styled-content> group showed: (1) significantly higher <jats:styled-content style=\"fixed-case\">pO<\/jats:styled-content><jats:sub>2<\/jats:sub> levels; (2) significantly greater human and murine <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>34\u2010positive endothelial areas; (3) significantly higher primordial follicle survival rates; (4) and significantly lower numbers of apoptotic follicles compared with the control group. Our research model demonstrates that by adding <jats:styled-content style=\"fixed-case\">ASC<\/jats:styled-content>s to a fibrin scaffold before OT transplantation, faster and better graft reoxygenation and revascularization may be obtained, resulting in increased follicle survival and reduced follicle apoptosis.<\/jats:p>","DOI":"10.1111\/aogs.13610","type":"journal-article","created":{"date-parts":[[2019,3,20]],"date-time":"2019-03-20T04:46:25Z","timestamp":1553057185000},"page":"665-671","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Translational research aiming to improve survival of ovarian tissue transplants using adipose tissue\u2010derived stem cells"],"prefix":"10.1111","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6331-3026","authenticated-orcid":false,"given":"Marie\u2010Madeleine","family":"Dolmans","sequence":"first","affiliation":[{"name":"Research Center in Gynecology Institute 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