{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T10:55:43Z","timestamp":1761562543476,"version":"3.40.5"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,9,10]],"date-time":"2019-09-10T00:00:00Z","timestamp":1568073600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Obstet Gynecol Scand"],"published-print":{"date-parts":[[2020,1]]},"abstract":"Abstract<\/jats:title>Introduction<\/jats:title>Bcl\u20102\u2010associated athanogene 3 (BAG<\/jats:styled-content>3) is a protein involved in apoptosis and stress response, which is overexpressed in invasive cervical cancer. However, nothing is known about BAG<\/jats:styled-content>3 expression in precancerous lesions of the uterine cervix. We aimed to evaluate the expression of BAG<\/jats:styled-content>3 in cervical intraepithelial neoplasia\/squamous intraepithelial lesions (CIN<\/jats:styled-content>\/SIL<\/jats:styled-content>).<\/jats:p><\/jats:sec>Material and methods<\/jats:title>Forty patients (16 CIN<\/jats:styled-content>1\/L\u2010SIL<\/jats:styled-content>, 11 CIN<\/jats:styled-content>2\/H\u2010SIL<\/jats:styled-content> and 13 CIN<\/jats:styled-content>3\/H\u2010SIL<\/jats:styled-content>) were assessed by immunohistochemistry for BAG<\/jats:styled-content>3. The intensity of BAG<\/jats:styled-content>3 expression was categorized as null, minimal, weak, moderate or strong. The association of BAG<\/jats:styled-content>2 intensity of expression with the grade of dysplasia was assessed using Chi\u2010square test (significant P<\/jats:italic> value <0.05).<\/jats:p><\/jats:sec>Results<\/jats:title>In all normal controls, BAG<\/jats:styled-content>3 expression was negative. In L\u2010SIL<\/jats:styled-content> specimens, BAG<\/jats:styled-content>3 expression was confined to the basal third of the epithelium, with an intensity minimal in nine cases (56.3%), weak in six (37.5%) and strong in one (6.3%). In H\u2010SIL<\/jats:styled-content> specimens, BAG<\/jats:styled-content>3 expression involved also the two upper thirds of the epithelium, with an intensity moderate in 13 cases (54.2%; 8 CIN<\/jats:styled-content>2 and 5 CIN<\/jats:styled-content>3) and strong in 11 cases (45.8%; 3 CIN<\/jats:styled-content>2 and 8 CIN<\/jats:styled-content>3). The distribution of BAG<\/jats:styled-content>3 expression correlated perfectly with the grade of dysplasia (P<\/jats:italic>\u00a0=\u00a00.0); a moderate\/strong expression of BAG<\/jats:styled-content>3 was significantly associated with H\u2010SIL<\/jats:styled-content> (P<\/jats:italic>\u00a0<\u00a00.0001), with no significant difference between CIN<\/jats:styled-content>2 and CIN<\/jats:styled-content>3 (P<\/jats:italic>\u00a0=\u00a00.1228).<\/jats:p><\/jats:sec>Conclusions<\/jats:title>In CIN<\/jats:styled-content>\/SIL<\/jats:styled-content>, both distribution and intensity of BAG<\/jats:styled-content>3 expression correlate directly with the grade of dysplasia, supporting the involvement of BAG<\/jats:styled-content>3 in all phases of cervical carcinogenesis and its possible diagnostic and prognostic role in cervical premalignant lesions.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aogs.13716","type":"journal-article","created":{"date-parts":[[2019,8,24]],"date-time":"2019-08-24T03:53:59Z","timestamp":1566618839000},"page":"99-104","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["BAG<\/scp>3 expression correlates with the grade of dysplasia in squamous intraepithelial lesions of the uterine cervix"],"prefix":"10.1111","volume":"99","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5443-2333","authenticated-orcid":false,"given":"Antonio","family":"Raffone","sequence":"first","affiliation":[{"name":"Gynecology and Obstetrics Unit Department of Neuroscience, Reproductive Sciences and Dentistry School of Medicine University of Naples Federico II Naples Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4002-1618","authenticated-orcid":false,"given":"Antonio","family":"Travaglino","sequence":"additional","affiliation":[{"name":"Anatomic Pathology Unit Department of Advanced Biomedical Sciences School of Medicine University of Naples Federico II Naples Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"D'Antonio","sequence":"additional","affiliation":[{"name":"SS. Giovanni di Dio e Ruggi d\u2019 Aragona University Hospital, Schola Medica Salernitana University of Salerno Hospital Salerno Italy"},{"name":"Department of Medicine, Surgery and Dentistry Schola Medica Salernitana University of Salerno Baronissi, Salerno Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margot","family":"De Marco","sequence":"additional","affiliation":[{"name":"Department of Medicine, Surgery and Dentistry Schola Medica Salernitana University of Salerno Baronissi, Salerno Italy"},{"name":"BIOUNIVERSA s.r.l. University of Salerno Baronissi, Salerno Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miriam","family":"Caccese","sequence":"additional","affiliation":[{"name":"Department of Medicine, Surgery and Dentistry Schola Medica Salernitana University of Salerno Baronissi, Salerno Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Massimo","family":"Mascolo","sequence":"additional","affiliation":[{"name":"Anatomic Pathology Unit Department of Advanced Biomedical Sciences School of Medicine University of Naples Federico II Naples Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luigi","family":"Insabato","sequence":"additional","affiliation":[{"name":"Anatomic Pathology Unit Department of Advanced Biomedical Sciences School of Medicine University of Naples Federico II Naples Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pio","family":"Zeppa","sequence":"additional","affiliation":[{"name":"SS. 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